Polyphenols composition from leaves of Cuphea spp. and inhibitor potential, in vitro, of angiotensin I-converting enzyme (ACE)

Santos, Marí Castro; Toson, Natally S.B.; Pimentel, M.C.B.; Bordignon, Sérgio A. L.; Mendez, Andreas Sebastian Loureiro; Henriques, Amélia T.

doi:10.1016/j.jep.2020.112781

Cited by 24 publications

(31 citation statements)

References 45 publications

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“…Meanwhile, functional analysis is required to confirm the antihypertensive activity of peptides in the fermented sample that contain sequences of already known antihypertensive peptides at their C and N terminals. Our findings are similar to other studies that reported that phenolic compounds [ 50 , 51 ] and bioactive peptides [ 52 ] in food can inhibit ACE activity.…”

Section: Resultssupporting

confidence: 93%

Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds

Daliri

Ofosu

Chelliah

et al. 2020

Foods

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Enzyme treatment and fermentation of cereals are known processes that enhance the release of bound bioactive compounds to make them available for bioactivity. In this study, we tested the angiotensin converting enzyme (ACE) inhibitory ability of destarched rice, Prozyme 2000p treated destarched rice (DP), and fermented DP samples. Prozyme 2000p treatment increased the ACE inhibitory ability from 15 ± 5% to 45 ± 3%. Fermentation of the Prozyme 2000p treated samples with Enterococcus faecium EBD1 significantly increased the ACE inhibitory ability to 75 ± 5%, while captopril showed an ACE inhibition of 92 ± 4%. An untargeted metabolomics approach using Ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of vitamins, phenolic compounds, antioxidant peptides, DPP IV inhibitory peptides, and antihypertensive peptides in the fermented samples which may account for its strong ACE inhibition. Although fermented DP had decreased fatty acid levels, the amount of essential amino acid improved drastically compared to destarched rice. Our results show that fermenting Prozyme-treated destarched rice with Enterococcus faecium EBD1 generates abundant bioactive compounds necessary for developing antihypertensive functional foods.

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Section: Resultssupporting

confidence: 93%

Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds

Daliri

Ofosu

Chelliah

et al. 2020

Foods

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“…In this study, flavonoid bioactive substances listed in Table 1 were shown to be abundant in SG extract. Flavonoids, a large class of polyphenols, have contributed to health maintenance, since they exert antioxidant, inflammatory, anti-hypertensive, and anti-cancer activities [ 27 , 28 ]. Thus, the biological effects of SG extract on L-NAME-induced high blood pressure in rats will be discussed further.…”

Section: Discussionmentioning

confidence: 99%

Syzygium gratum Extract Alleviates Vascular Alterations in Hypertensive Rats

et al. 2020

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Background and Objectives: Syzygium gratum (SG) is a local vegetable and widely consumed in Thailand. Previously, a strong antioxidative effect of SG extract has been reported. The effects of SG extract on hypertension have remained unknown. The effect of SG aqueous extract on blood pressure and vascular changes were examined in L-NAME-induced hypertensive rats (LHR), and its potential active constituents were also explored. Materials and Methods: Male Sprague Dawley rats were allocated to control, L-NAME (40 mg/kg/day), L-NAME + SG (100, 300, 500 mg/kg/day), or captopril (5 mg/kg/day) groups. The components of SG extract were analyzed. Results: The analysis of aqueous SG extract was carried out using HPLC-Mass spectroscopy, and phenolic compounds could be identified as predominant components which might be responsible for its antihypertensive effects observed in the LHR model (p < 0.05). Additionally, SG extract also improved vascular responses to acetylcholine and decreased vascular remodeling in LHR (p < 0.05). Enhancements of eNOS expression and plasma nitric oxide metabolite levels, and attenuation of angiotensin converting enzyme (ACE) activity and plasma angiotensin II levels were observed in the LHR group treated with SG. Moreover, SG exhibited strong antioxidant activities by reducing vascular superoxide generation and systemic malondialdehyde in LHRs. Captopril suppressed high blood pressure and alleviated vascular changes and ACE activity in LHRs, similar to those of the SG extract (p < 0.05). Conclusion: Our results suggest that the SG extract exhibited antihypertensive effects, which is relevant to alleviation of vascular dysfunction and vascular remodeling of LHRs. These effects might be mediated by phenolic compounds to inhibit ACE activity and scavenge reactive oxygen species in LHR.

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“…The extracts from the leaves of C. lindmaniana and C. urbaniana were prepared according to Santos et al [2,3] . performed by ultrasound‐assisted method, where the vegetal material was submitted to an ultrasonic bath (40 kHz, 132 W), [15] under the following conditions: particle size at≤180 μm; plant:solvent ratio 1: 20 (w/v); 40 % ethanol and time 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Antihypertensive activity was measured by determination of ACE‐inhibition according to the method of Tabatabei et al [31] . with few modifications according to Santos et al [2] . In each test, the assay was buffered with 50 mM Tris‐HCl (pH 8.3).…”

Section: Methodsmentioning

confidence: 99%

“…A raised of reports describe the inhibitory effect of phenolic compounds against human enzymes, [2,5,6,7] thereby potential from phenolic compounds against key‐enzymes, such as angiotensin I‐converting enzyme, cyclooxygenases (COXs), and 5‐lipoxygenase (5‐LOX) mainly, are studied [5] . Besides, the neutrophils are the primary cells recruited to inflamed sites during the immune response to tissue damage and/or infection for an effective response.…”

Section: Introductionmentioning

confidence: 99%

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Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Santos

Soares

Beltrame

et al. 2021

Chemistry & Biodiversity

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The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves. The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization. The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001 -10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils. The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition. From the nineteen proposed compounds, six of them are described for the first time in this genus. The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations. The ACE-inhibition presented results ranging from 19.58 to 22.82 %. In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential. Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.

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Polyphenols composition from leaves of Cuphea spp. and inhibitor potential, in vitro, of angiotensin I-converting enzyme (ACE)

Cited by 24 publications

References 45 publications

Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds

Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds

Syzygium gratum Extract Alleviates Vascular Alterations in Hypertensive Rats

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

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