Polyphenolic Profiling, Quantitative Assessment and Biological Activities of Tunisian Native Mentha rotundifolia (L.) Huds.

Yahia, Imen Ben Haj; Ciavatta, Maria Letizia; Ligresti, Alessia; Jaouadi, Rym; Boussaïd, Mohamed; Cutignano, Adele

doi:10.3390/molecules24132351

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…In addition to caffeic acid (peak 3) and rosmarinic acid (peak 21) this research has shown the presence of ten other caffeic acid esters (peaks 1,7,15,19,22,24,25,27,30,34) known as lithospermic acids or salvianolic acids. Caffeic acid, rosmarinic acid, lithospermic acid A (peak 22), and lithospermic acid B (peak 24) were identified using authentic standards or herbal reference materials (Salvia miltiorrhiza Bunge, Melissa officinalis L.) and literature data [32,34,35]. Interpretation of data from MS/MS experiments for remaining peppermint caffeetannins is presented in Table 2.…”

Section: Resultsmentioning

confidence: 99%

“…Flavanones (peaks 6, 8, 11, 13, 16, 29, 32, 35) were detected on the U(H)PLC chromatograms between 11.13 and 16.35 min. Six peaks (6, 13,16,29,32,35) were identified as eriocitrin, narirutin, hesperidin, eriodictyol, naringenin, and hesperetin, respectively. Their fragmentation, t R and UV-Vis spectra corresponded to proper parameters of authentic standards and literature data [38].…”

Section: Resultsmentioning

confidence: 99%

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Analysis of Polyphenolic Composition of a Herbal Medicinal Product—Peppermint Tincture

et al. 2019

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The pharmacological activity of peppermint leaf (Menthae piperitae folium) for medical use is mainly attributed to the presence of essential oil, which, according to the European Pharmacopoeia (Ph. Eur.), should constitute not less than 12 mL/kg of raw material. The content of polyphenols in peppermint-based preparations, except peppermint leaf dry extract, has not yet been considered as an essential parameter in the pharmacopeial assessment of peppermint quality. This study concerns the evaluation of the presence of representatives of polyphenolic compounds in 23 commercial peppermint tinctures (ethanolic extracts) purchased in pharmacies in Poland. The non-volatile polyphenolic fraction was investigated, and the presence of flavonoids and phenolic acids was quantified. High performance liquid chromatography coupled with a diode-array detector (HPLC-DAD) and an electrospray ionization mass spectrometer (U(H)PLC-ESI-MS) were used in the experiment. The study showed that eriocitrin, luteolin-7-O-rutinoside, and rosmarinic acid were the main polyphenolic components of the peppermint tinctures, as previously reported for peppermint leaf. Despite this, the research shows the extremely diverse content of the mentioned compounds in analyzed commercial medicinal products. In light of these results, it seems that the pharmacopeial assessment for the peppermint leaf (Ph. Eur.) and peppermint tincture (Polish Pharmacopoeia (FP)) requires correction and supplementation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Analysis of Polyphenolic Composition of a Herbal Medicinal Product—Peppermint Tincture

et al. 2019

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“…Mint species have also been used for medicinal aims, such as in infusions or tinctures for the treatment of intestinal colic, liver disorders, gastritis, and jaundice, as well as for headaches and migraine [ 6 ]. Mint extracts were found to contain a wealth of compounds, collectively named terpenoids and polyphenols, which include phenolic acids, flavones, and flavanols, in their free forms or as glycoconjugates [ 7 ]. Similar components were also found in the leaves and flowers of small wild plants growing in Saudi Arabia named Lantana camara , Anvillea garcinii , and Strychnos nux-vomica [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

et al. 2022

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Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

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“…Polyphenols have been found to possess high antioxidant activity as well as a plethora of favourable benefits to human health (Queen and Tollefsbol 2010 ; Ben Haj Yahia et al. 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…From our result, BPFRF, quercetin, and ascorbic acid caused a concentration-dependent increase in the inhibition of the DPPH radical Figure 4. DPPH is a stable nitrogen-based free radical that functions as a substrate of radical-trapping reactions in this method (Assefa et al 2016;Ben Haj Yahia et al 2019). The BPFRF caused a fast conversion of the purple-colored DPPH radical to pale yellow.…”

Section: Identification and Characterization Of Bpfrf By Uplc-esi-qtof-msmentioning

confidence: 99%

UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum ; antioxidant properties, anticholinesterase activity and in silico studies

Ogidigo

Anosike

Joshua

et al. 2021

Pharmaceutical Biology

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Context Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments. Objectives This study characterizes the constituents of B. pinnatum flavonoid-rich fraction (BPFRF) and investigates their antioxidant and anticholinesterase activity using in vitro and in silico approaches. Materials and methods Methanol extract of B. pinnatum leaves was partitioned to yield the ethyl acetate fraction. BPFRF was isolated from the ethyl acetate fraction and purified. The constituent flavonoids were structurally characterized using UPLC-PDA-MS 2 . Antioxidant activity (DPPH), Fe 2+ -induced lipid peroxidation (LP) and anticholinesterase activity (Ellman’s method) of the BPFRF and standards (ascorbic acid and rivastigmine) across a concentration range of 3.125–100 μg/mL were evaluated in vitro for 4 months. Molecular docking was performed to give insight into the binding potentials of BPFRF constituents against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Results UPLC-PDA-MS 2 analysis of BPFRF identified carlinoside, quercetin (most dominant), luteolin, isorhamnetin, luteolin-7-glucoside. Carlinoside was first reported in this plant. BPFRF significantly inhibited DPPH radical (IC 50 = 7.382 ± 0.79 µg/mL) and LP (IC 50 = 7.182 ± 0.60 µg/mL) better than quercetin and ascorbic acid. Also, BPFRF exhibited potent inhibition against AChE and BuChE with IC 50 values of 22.283 ± 0.27 µg/mL and 33.437 ± 1.46 µg/mL, respectively compared to quercetin and rivastigmine. Docking studies revealed that luteolin-7-glucoside, carlinoside and quercetin interact effectively with crucial amino acid residues of AChE and BuChE through hydrogen bonds. Discussion and conclusions BPFRF possesses an excellent natural source of cholinesterase inhibitor and antioxidant. The material could be further explored for the potential treatment of oxidative damage and cholinergic dysfunction in Alzheimer’s disease.

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Polyphenolic Profiling, Quantitative Assessment and Biological Activities of Tunisian Native Mentha rotundifolia (L.) Huds.

Cited by 16 publications

References 39 publications

Analysis of Polyphenolic Composition of a Herbal Medicinal Product—Peppermint Tincture

Analysis of Polyphenolic Composition of a Herbal Medicinal Product—Peppermint Tincture

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum ; antioxidant properties, anticholinesterase activity and in silico studies

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