Polyphenolic Extracts from Spent Coffee Grounds Prevent H2O2-Induced Oxidative Stress in Centropomus viridis Brain Cells

Abstract

Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H2O2, in C. viridis brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of C. viridis brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM H2O2 exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in C. viridis brain cells by stimulating the activity of SOD and CAT.

Dietary Selenium Alleviated Mouse Liver Oxidative Stress and NAFLD Induced by Obesity by Regulating the KEAP1/NRF2 Pathway

“…H 2 O 2 is one of the endogenous ROS [ 13 ]. Therefore, H 2 O 2 is a common oxidative stress inducer.…”

“…Selenium (Se) as an essential micronutrient in animals; it plays a vital role in several regulatory and metabolic functions of the body, such as antioxidant, anti-cancer, and detoxification [ 11 , 12 ]. In the liver, dietary selenium is metabolized to selenide, which is subsequently used for the synthesis of selenoproteins [ 13 ]. The major selenoproteins in the liver include SEPP1 and glutathione peroxidase1 (GPX1) [ 14 ].…”