Polyphenolic extract from extra virgin olive oil inhibits the inflammatory response in IL-1<i>β</i>-activated synovial fibroblasts

Rosillo, María Ángeles; Alarcón‐de‐la‐Lastra, Catalina; Castejón, María Luisa; Montoya, Tatiana; Cejudo-Guillén, Marta; Sánchéz-Hidalgo, Marina

doi:10.1017/s0007114518002829

Cited by 32 publications

(23 citation statements)

References 48 publications

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“…The inhibition of the NF-kB pathway, iNOS induction and the reduction in IL-6, IL-8 and NO levels were detected after oxysterol stimulation in the presence of the phenolic extract. HT In a rheumatoid arthritis model, the EVOO phenolic extracts showed joint protective properties and reduced proinflammatory mediators by the inhibition of MAPK and NF-κB signaling in activated synovial fibroblasts [124]. In this model, a polyphenolic extract also inhibited IL-1β-induced matrix metalloproteinases, TNF-α and IL-6 production, as well as IL-1β-induced cyclo-oxygenase-2 (COX-2) and microsomal PGE synthase-1 (mPGES-1) [125].…”

Section: Anti-inflammatory Properties Of Olive Polyphenols: Molecular Mechanismsmentioning

confidence: 96%

“…This healthy effect results largely from the anti-inflammatory power of the polyphenolic compounds that is expressed by various mechanisms such as antioxidant activity (see previous paragraph) and the modulation of signaling pathways and transcriptional events (Figure 3). In a rheumatoid arthritis model, the EVOO phenolic extracts showed joint protective properties and reduced proinflammatory mediators by the inhibition of MAPK and NF-κB signaling in activated synovial fibroblasts [124]. In this model, a polyphenolic extract also inhibited IL-1β-induced matrix metalloproteinases, TNF-α and IL-6 production, as well as IL-1β-induced cyclo-oxygenase-2 (COX-2) and microsomal PGE synthase-1 (mPGES-1) [125].…”

Section: Anti-inflammatory Properties Of Olive Polyphenols: Molecular Mechanismsmentioning

confidence: 98%

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Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties

et al. 2021

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Oxidative stress and inflammation triggered by increased oxidative stress are the cause of many chronic diseases. The lack of anti-inflammatory drugs without side-effects has stimulated the search for new active substances. Plant-derived compounds provide new potential anti-inflammatory and antioxidant molecules. Natural products are structurally optimized by evolution to serve particular biological functions, including the regulation of endogenous defense mechanisms and interaction with other organisms. This property explains their relevance for infectious diseases and cancer. Recently, among the various natural substances, polyphenols from extra virgin olive oil (EVOO), an important element of the Mediterranean diet, have aroused growing interest. Extensive studies have shown the potent therapeutic effects of these bioactive molecules against a series of chronic diseases, such as cardiovascular diseases, diabetes, neurodegenerative disorders and cancer. This review begins from the chemical structure, abundance and bioavailability of the main EVOO polyphenols to highlight the effects and the possible molecular mechanism(s) of action of these compounds against inflammation and oxidation, in vitro and in vivo. In addition, the mechanisms of inhibition of molecular signaling pathways activated by oxidative stress by EVOO polyphenols are discussed, together with their possible roles in inflammation-mediated chronic disorders, also taking into account meta-analysis of population studies and clinical trials.

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Section: Anti-inflammatory Properties Of Olive Polyphenols: Molecular Mechanismsmentioning

confidence: 96%

Section: Anti-inflammatory Properties Of Olive Polyphenols: Molecular Mechanismsmentioning

confidence: 98%

Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties

et al. 2021

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“…It has been evidenced that NF-κB is involved in abnormal apoptosis and proliferation of RA fibroblast-like synovial cells [ 23 ]. In RA, NF-κB is over-expressed in the inflamed synovium [ 24 ], where its activity may enhance recruitment of inflammatory cells and production of proinflammatory mediators such as IL-1β, IL-6, IL-8 and TNF-α [ 25 ]. Therefore, SGE might attenuate the RA through inhibiting the synovial hyperplasia and inflammation by blocking NF-κB signal.…”

Section: Discussionmentioning

confidence: 99%

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

et al. 2020

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Background: Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extract (SGE) on collagen-induced arthritic rats and interleukin (IL)-1β-stimulated human synovial SW982 cells. Methods: Rats were induced to arthritis by collagen for 15 days and then received SGE treatment for 35 days. The body weight and arthritis severity score of the rats were monitored weekly. At the end of the experiment, the radiographic and histological changes of rats' hind paw were obtained; the serum C-reactive protein was detected by enzyme-linked immunosorbent assay (ELISA); the expression levels of interleukin (IL)-1β, IL6 and IL-10 in the joint muscles were determined by ELISA and immunohistochemical staining; and the level of regulatory T cells (Tregs) in the spleen was detected using flow cytometry. In addition, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were used to evaluate the proliferation of SW982 synovial cells. ELISA, western blot and immunofluorescence staining were used to investigate the anti-inflammatory mechanisms of SGE on IL-1β-induced SW982 cells and joint muscles of CIA rats. Results: SGE attenuated the collagen-induced hind paw swelling, cartilage damage and bone erosion. SGE inhibited the synovial hyperplasia to the articular cavity in the toe joint and ankle. Moreover, SGE decreased the production of C-reactive protein in serum and the expression of IL-6, IL-1β, cyclooxygenase-2 (COX-2) and phosphorylation of NF-κB p65 in the joint muscles. SGE also recovered the decreased Tregs. Results from the in vitro experiments showed that SGE not only inhibited the proliferation and migration of human synovial cell but also inhibited the IL-1β-induced expression of IL-6 and IL-8. Similarly, SGE inhibited the activation of NF-κB and the expression of COX-2. Conclusions: SGE attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation.

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“…In RA, NF-κB is over-expressed in the inflamed synovium [23], where its activity may enhance recruitment of inflammatory cells and production of proinflammatory mediators such as IL-1β, IL-6, IL-8 and TNF-α [24]. Therefore, SGE might inhibit the proliferation and inflammation on IL-1β-induced SW982 by blocking NF-κB.…”

Section: Discussionmentioning

confidence: 99%

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

Qiu¹,

Linghu

Zhang

et al. 2020

Preprint

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Background Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extract (SGE) on collagen-induced arthritic rats and interleukin (IL)- 1β-stimulated human synovial SW982 cells. Methods Rats were induced to arthritis by collagen for 15 days and then received SGE treatment for 35 days. The body weight and arthritis severity score of the rats were monitored weekly. At the end of the experiment, the radiographic and histological changes of rats’ hind paw were obtained; the cytokines expression in serum and joint muscles were determined by enzyme-linked immunosorbent assay (ELISA); and the level of regulatory T cells (Tregs) in the spleen was detected using flow cytometry. In addition, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were used to evaluate the proliferation of SW982 synovial cells. ELISA, western blot and immunofluorescence staining were used to investigate the anti-inflammatory mechanisms of SGE on IL-1β-induced SW982 cells. Results SGE attenuated the collagen-induced hind paw swelling, cartilage damage and bone erosion. SGE inhibited the synovial hyperplasia to the articular cavity in the toe joint and ankle. Moreover, SGE decreased the production of C-reactive protein in serum and the expression of IL-6 and IL-1β in the joint muscle. SGE also recovered the decreased Tregs. Results from the in vitro experiments showed that SGE not only inhibited the proliferation and migration of human synovial cell but also inhibited the IL-1β-induced expression of IL-6 and IL-8. Besides, SGE inhibited the activation of nuclear transcription factor-kappa B and the expression of cyclooxygenase-2. Conclusions SGE attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation.

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Polyphenolic extract from extra virgin olive oil inhibits the inflammatory response in IL-1β-activated synovial fibroblasts

Cited by 32 publications

References 48 publications

Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties

Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

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