Polyphenolic contents, antioxidant activities and UPLC–ESI–MS analysis of Haplophyllum tuberculatum A. Juss leaves extracts

Hamdi, Abdelaziz; Viane, Johan; Mahjoub, Mohamed Ali; Majouli, Kaouther; Gad, Mahmoud Hussein Hefni; Kharbach, Mourad; Demeyer, Kristiaan; Marzouk, Zohra; Heyden, Yvan Vander

doi:10.1016/j.ijbiomac.2017.08.107

Cited by 26 publications

(29 citation statements)

References 27 publications

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“…The correlation between the content of components and antioxidant activity of extracts was explored by reported multivariate regression analysis [33]. All statistical analyses were performed with the SPSS Statistics (SPSS vision 21, IBM, Camp Takajo, NY, USA).…”

Section: Statistical Analysis Of Content and Antioxidant Activitymentioning

confidence: 99%

Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Guo

Chen

et al. 2020

Molecules

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Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and PN, LG, and PA leaves were analyzed. PN showed four times as much chlorogenic acid (CA) than the other two, and contained the most isoorientin (iso-ORI) and isovitexin (iso-VIT) as well. The PA presented the most orientin (ORI) and LG covered a majority of cynaroside (CYN). We measured the antioxidant activity by scavenging the stable 2,2-diphenyl-1-pyridinohydrazinyl (DPPH) free radicals, and found that Luteolin (inhibitory concentration (IC)50 = 0.42 µM, LUT) and CYN (IC50 = 0.43 µM) showed 2–3 times higher antioxidant activity than iso-ORI (IC50 = 0.81 µM), ORI (IC50 = 0.84 µM), and other related antioxidant standards such as trolox (IC50 = 0.97 µM) and ascorbic acid (IC50 = 0.93 µM, VC). Among extracts, PN and PA showed considerable antioxidant activity, which was related well with the contents of CA, iso-ORI, and iso-VIT (p < 0.05). This study firstly provides evidence for functional antioxidant compounds of bamboo leaves based on statistical analysis of the HPLC analysis and DPPH assay, and it lays a foundation for its further development or utilization.

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Section: Statistical Analysis Of Content and Antioxidant Activitymentioning

confidence: 99%

Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Guo

Chen

et al. 2020

Molecules

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“…The precursor ion at m/z 625.1775 (C 28 H 32 O 16 ) exhibited neutral losses of rhamnosyl, glucosyl, and methyl groups, respectively, yielding fragment ions at m/z 479.1149 ([ M −rhamnosyl+H] + ), 317.0620 ([aglycone+H] + ), and 302.0349 ([aglycone−CH 3 +H] + ). This led to the tentative identification of peak 3 as isorhamnetin rutinoside ( Figure a ), a constituent previously reported in various Haplophyllum species, such as H. pedicellatum , H. robustum , and H. glabrum …”

Section: Resultsmentioning

confidence: 62%

“…This led to the tentative identification of peak 3 as isorhamnetin rutinoside (Figure 2a), a constituent previously reported in various Haplophyllum species, such as H. pedicellatum, H. robustum, and H. glabrum. [17]…”

Section: Hplc-dad-esi-q-tof-ms/ms Metabolite Profilingmentioning

confidence: 99%

Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS) and Neurobiological Potential of Haplophyllum sahinii and H. vulcanicum Extracts

Karahisar

Tuğay

Orhan

et al. 2019

Chemistry & Biodiversity

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In the current study, the ethanol extracts of flower, stem, and root parts of two endemic Turkish species, e. g., Haplophyllum sahinii O. Tugay & D. Ulukuş and H. vulcanicum Boiss. & Heldr., were screened against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) associated with Alzheimer's disease as well as tyrosinase (TYR) linked to Parkinson's disease using ELISA microplate assay at 200 μg/mL. Among the extracts, the highest inhibition was caused by the stem extract of H. sahinii against BChE (IC50=64.93±1.38 μg/mL). Consistently, all of the extracts were found to exert a selective inhibition towards BChE to some extent. It was only the root extract of H. vulcanicum that could inhibit AChE at low level (IC50=203.18±5.33 μg/mL). None of the extracts displayed an inhibition over 50 % against TYR. Metabolite profiling of the extracts was achieved by a highly hyphenated liquid chromatographic mass spectrometric technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS), which revealed the presence of furoquinoline (β‐fagarine, γ‐fagarine) and amide (tubasenicine, tubacetine) alkaloids; furano‐ (rutamarin), pyrano‐ (xanthyletine), and geranyloxy coumarins; phenylpropanoid (secoisolariciresinol), arylnaphthalene (mono‐O‐acetyldiphyllin apioside), and dibenzylbutyrolactone (kusunokinin, haplomyrfolin) lignans. Several important differences were observed between the extracts analyzed. β‐Fagarine was the major alkaloid in H. vulcanicum, whereas γ‐fagarine was present only in the roots of both Haplophyllum species; moreover, secoisolariciresinol and secoisolariciresinol dimethyl ether were the main lignans in the stems and flowers. This is the first study identifying ChE and TYR inhibitory effect and metabolic profiles of H. vulcanicum and H. sahinii.

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“…In Sudan, the plant is locally known as Haza, and the aerial parts have been used traditionally to treat malaria, asthma, kidney diseases, gynecological and bowel disorders [10,11]. Anti-inflammatory, antioxidant, antibacterial, and antifungal activities have been reported [10][11][12], and alkaloids, flavonoids, coumarins and lignans have been identified [13][14][15]. The methanolic extract of the aerial part and roots of H. tuberculatum possessed activity against P. falciparum [16], and Justicidin A was found active [17].…”

Section: Introductionmentioning

confidence: 99%

Lignans, Amides, and Saponins from Haplophyllum tuberculatum and Their Antiprotozoal Activity

et al. 2020

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A screening of Sudanese medicinal plants for antiprotozoal activities revealed that the chloroform and water fractions of the ethanolic root extract of Haplophyllum tuberculatum exhibited appreciable bioactivity against Leishmania donovani. The antileishmanial activity was tracked by HPLC-based activity profiling, and eight compounds were isolated from the chloroform fraction. These included lignans tetrahydrofuroguaiacin B (1), nectandrin B (2), furoguaiaoxidin (7), and 3,3′-dimethoxy-4,4′-dihydroxylignan-9-ol (10), and four cinnamoylphenethyl amides, namely dihydro-feruloyltyramine (5), (E)-N-feruloyltyramine (6), N,N′-diferuloylputrescine (8), and 7′-ethoxy-feruloyltyramine (9). The water fraction yielded steroid saponins 11–13. Compounds 1, 2, and 5–13 are reported for the first time from Haplophyllum species and the family Rutaceae. The antiprotozoal activity of the compounds plus two stereoisomeric tetrahydrofuran lignans—fragransin B2 (3) and fragransin B1 (4)—was determined against Leishmania donovani amastigotes, Plasmodium falciparum, and Trypanosoma brucei rhodesiense bloodstream forms, along with their cytotoxicity to rat myoblast L6 cells. Nectandrin B (2) exhibited the highest activity against L. donovani (IC50 4.5 µM) and the highest selectivity index (25.5).

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Polyphenolic contents, antioxidant activities and UPLC–ESI–MS analysis of Haplophyllum tuberculatum A. Juss leaves extracts

Cited by 26 publications

References 27 publications

Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS) and Neurobiological Potential of Haplophyllum sahinii and H. vulcanicum Extracts

Lignans, Amides, and Saponins from Haplophyllum tuberculatum and Their Antiprotozoal Activity

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