Polyphenolic compounds transition into protein-plant concentrates during the deposition of milk proteins by Plantago major L

Abstract

Introduction. Implementation of scientifically sound use principles of dairy and plant raw materials with functionaltechnological properties is relevant. Materials and methods. Normalized milk was used as the raw material to obtain protein concentrates. As a coagulant in the deposition of proteins used juice of direct extraction from the terrestrial part of Plantago major L. Identification and quantification of polyphenols and flavanoids in the samples of the plantain juice and the obtained whey was performed by high-performance liquid chromatography using system Prominence LC-20 Shimadzu (Japan). Comparisons were made with external standard samples. Results and discussion. The study of the qualitative composition and quantitative content of polyphenols and flavonoids in protein-plant concentrates obtained by deposition of milk proteins with the juice of direct extraction of the terrestrial part of Plantago major L were presented. The process took place at elevated temperatures. According to the analysis of absorption spectra of the experimental samples of juice from the plantain found that the maximum is at wavelengths of 225-350 nm. This result is correlated with the fact that of the 22 isolated flavonoids, 9 substances are 6oxyflavones, characterized by a maximum within the range of 255-285 nm. The difference between the content of polyphenols in Plantago major L. juice and whey indicates the degree of transition of polyphenolic compounds into protein-plant concentrates. The total content of flavonoids in all tested samples was equal to the content of substances that were similar to the standards of flavonoids (phenolic acids, catechins, flavonols, flavonones and flavones) with the exception of catechin-like substances. Flavonols are represented by the glycosides of myricetin. Naringin and hesperidin are part of flavonones. The flavones in the quantitative sense are of the lowest weight and are represented by luteolin and its glycosides and glycosides of apigenin. Phenolic acids content ranged from 12.36 mg/l for plantain juice and 0.07 mg/l for colored whey. Conclusions. The degree of polyphenolic compounds transition in protein-plant concentrate was determined at the level-77% of the total amount, of which 74% are flavonoids.

Optimization Method and Stability Test to Determinate Luteolin, Quercetin, Apigenin, and Sinensetin Levels in Herbal Medicines Using TLC-Densitometry

“…The research that has been carried out is limited to determining marker compounds in single herbal plants and has not been applied to the formulation of herbal medicinal preparations using the TLC-Densitometry method (Abdullah et al, 2012;Hossain & Ismail, 2016;Kuppusamy et al, 2017). Several analytical methods for determining luteolin, quercetin, apigenin, and sinensetin have been developed using the HPLC method (Kuppusamy et al, 2017;Grek et al, 2019;Ghiasian et al, 2021). however, this instrument is expensive and require expertise specialized in instrument operation.…”