Polyphenol-Rich Liupao Tea Extract Prevents High-Fat Diet-Induced MAFLD by Modulating the Gut Microbiota

Abstract

The modulation of gut microbiota dysbiosis might regulate the progression of metabolic-associated fatty liver disease (MAFLD). Here, we found that polyphenol-rich Liupao tea extract (PLE) prevents high-fat diet (HFD)-induced MAFLD in ApoE−/− male mice accompanied by protection of the intestinal barrier and downregulation of lipopolysaccharide (LPS)-related Toll-like receptor 4 (TLR4)-myeloid differentiation primary response 88 (MyD88) signaling in the liver. Fecal microbiome transplantation (FMT) from PLE-and-HFD-treated mice delayed MAFLD development significantly compared with FMT from HFD-treated mice. In this case, 16S rRNA gene sequencing revealed that Rikenellaceae and Odoribacter were significantly enriched and that Helicobacter was significantly decreased in not only the HFD+PLE group but also the HFD+PLE-FMT group. Furthermore, the level of 3-sulfodeoxycholic acid was significantly decreased in the HFD+PLE-FMT group compared with the HFD-FMT group. In conclusion, our data demonstrate that PLE could modulate the MAFLD phenotype in mice and that this effect is partly mediated through modulation of the gut microbiota.

Liubao tea extract ameliorates ovalbumin-induced allergic asthma by regulating gut microbiota in mice

“…25 Recent advances describe the positive effects of LBT extracts/ components against various diseases. [26][27][28] Nevertheless, the potential antiallergic effect and underlying regulatory pathways of LBTE on asthma continue to attract attention.…”

Tactics with Prebiotics for the Treatment of Metabolic Dysfunction-Associated Fatty Liver Disease via the Improvement of Mitophagy

“…Therefore, it is reasonable to consider that gut dysbiosis, resulting in the dysregulation of SCFA production, increases the susceptibility of liver injury. In fact, MAFLD pathogenesis is closely associated with gut dysbiosis [ 108 ]. The gut dysbiosis could accelerate the development of MAFLD due to abnormal levels of gut microbial metabolites and/or their effects on gut epithelial permeability [ 109 , 110 ].…”

A systemic review on Liubao tea: A time‐honored dark tea with distinctive raw materials, process techniques, chemical profiles, and biological activities

“…LBT also prevents NAFLD through improving intestinal barrier function and modifying the gut microbiota communities, especially by reducing the level of NAFLD‐related Allobaculum (Li, Mao et al., 2022). Besides, LBT extract prevented NAFLD through the downregulation of lipopolysaccharide‐related toll‐like receptor 4 (TLR4)–myeloid differentiation primary response 88 (MYD88) signaling (Tang et al., 2022).…”

“…Tang et al. (2022) proved the preventive effects of polyphenol‐rich LBT extract, which reversed intestinal barrier permeability and inhibited TLR4–MYD88 signaling in the liver. The key active components, including EGCG, EA, and caffeine, have been identified in LBT water extract for the anti‐obesity effects, targeting the PI3K‐Akt signaling, tumor necrosis factor signaling, and insulin resistance pathways (Wu et al., 2023).…”

“…Specifically, the abundance of butyrate‐related genera Rikenellaceae and Odoribacter increased, whereas those of intestinal permeability‐related Helicobacter genus decreased. In addition, LBT extract enriched microbial metabolites such as N ‐lauroyl cysteine, prolyltryptophan, and taurine, which regulated intestinal barrier and oxidative stress factors (Tang et al., 2022). Zhao et al.…”