Polyphenol oxidase (PPO) arm of catecholamine pathway catalyzes the conversion of L-tyrosine to L-DOPA in Mucuna pruriens (L.) DC var. pruriens: An integrated pathway analysis using field grown and in vitro callus cultures

Saranya, G.; Sruthi, D.; Jayakumar, K.S.; Jiby, M.V.; Nair, R. Aswati; Pillai, Padmesh P.; Jayabaskaran, Chelliah

doi:10.1016/j.plaphy.2021.06.053

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…Then the proposed pathway of dopamine in M. guilliermondii GXDK6 had been constructed successfully (as shown in Fig. 2 A) [ 29 ]. d -Erythrose 4-phosphate had been found to be converted into 3-deoxy-D-arabino-heptulosoate 7-phosphate (DAHP) under the action of 3-deoxy-7-phosphoheptulonate synthase (encoded by ARO4 ).…”

Section: Resultsmentioning

confidence: 99%

Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis

Sun,

Bai,

et al. 2024

Synthetic and Systems Biotechnology

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Section: Resultsmentioning

confidence: 99%

Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis

Sun,

Bai,

et al. 2024

Synthetic and Systems Biotechnology

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“…Peroxidases not only remove toxic hydrogen peroxide from tissues but also participate in phenolic compounds biosynthesis and make intermolecular bonds in cell wall organization at pathogen infection sites. In plants, increase in specific activity of peroxidases during pathogen attack may be due to their involvement in biosynthesis and lignification of phenolic compounds and their accumulation in the cell wall making it an effective barrier against pathogens [74]. In the presence of H 2 O 2 , peroxidase carries out oxidation of phenolic compounds, the products of this oxidation accumulate in infected tissues of plants and provide resistance against pathogens.…”

Section: Area Under Disease Progressive Curvementioning

confidence: 99%

In vitro Induced Mutagenesis for Early Blight (<i>Alternaria solani</i>) Resistance in Potato (<i>Solanum tuberosum</i> L.)

Naveed,

Naz,

Khalid

et al. 2023

Pol. J. Environ. Stud.

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In Pakistan, potato is grown over an area of 0.20 million hectares having annual production of 4.58 million tons and average yield of 22.9 tons/hectare [1], which is far below than other potato growing countries of the world. About 8% of the area for potato cultivation is increasing annually. Moreover, due to susceptibility of disease and abrupt climatic change, the yield potential of varieties under cultivation is deteriorating. Various pathogens can attack potato crops at any stage of growth leading to yield losses (even upto 100% loss). Among them A. solani (asexual plant-pathogenic species) causing early blight disease in potato is very common and can spread from one crop to other through its debris or from neighboring infected fields. A. solani is polycyclic, i.e. it can infect plants repeatedly in one growing season. This disease

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“…Oviedo-Silva et al ( 2018) also showed L-DOPA concentrations (125 mg g −1 DW) in faba bean sprouts using the HPTLC (high performance thin layer chromatography) -UV method. HPLC or HPTLC is common and widely used for quantifying L-DOPA (Hu et al 2015;Saranya et al 2021;Shetty et al 2002). The quantitative NMR technique has also been used to quantify the L-DOPA of the sprouts (Fernandez-Pastor et al 2019;Okumura et al 2016).…”

mentioning

confidence: 99%

The simple and rapid quantification method for L-3,4-dihydroxyphenylalanine (L-DOPA) from plant sprout using liquid chromatography-mass spectrometry

Yumoto

Yanagihara

Asahina

2022

Plant Biotechnology

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L-3,4-dihydroxyphenylalanine (L-DOPA) is one of the important secondary metabolites of plants and has been used for various purposes, such as in clinical treatment for Parkinson's disease and dopamine-responsive dystonia. In plants, L-DOPA is a precursor of many alkaloids, catecholamines, and melanin; the L-DOPA synthesis pathway is similar to that in mammals. L-DOPA acts as an allelochemical, has an important role in several biological processes, such as stress response and metabolism, in plants. L-DOPA is widely used in the clinical treatment as well as a dietary supplement or psychotropic drug, understanding of biosynthesis of L-DOPA in plant could lead to a stable supply of L-DOPA. This paper describes an improved method for simple and rapid quantification of L-DOPA content using liquid chromatography-tandem mass spectrometry. The standard quantitative methods for L-DOPA require multiple purification steps or relatively large amounts of plant material. In our improved method, quantification of L-DOPA was possible with extract of one-two pieces of cotyledon without any partitioning or column for purification. The endogenous L-DOPA (approximately 4,000 µg g −1 FW (fresh weight)) could be detected from the one pieces of cotyledon of the faba bean sprout using this method. This method was also effective for samples with low endogenous amounts of L-DOPA such as broccoli, Japanese white radish, pea, and red cabbage sprouts. Therefore, this improved method will allow to measurement of L-DOPA content easily and accurately from a small amount of plant tissue and contribute to understanding biosynthesis, catabolism, and transport of L-DOPA.

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Polyphenol oxidase (PPO) arm of catecholamine pathway catalyzes the conversion of L-tyrosine to L-DOPA in Mucuna pruriens (L.) DC var. pruriens: An integrated pathway analysis using field grown and in vitro callus cultures

Cited by 10 publications

References 33 publications

Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis

Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis

In vitro Induced Mutagenesis for Early Blight (<i>Alternaria solani</i>) Resistance in Potato (<i>Solanum tuberosum</i> L.)

The simple and rapid quantification method for L-3,4-dihydroxyphenylalanine (L-DOPA) from plant sprout using liquid chromatography-mass spectrometry

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