Polyphenol-hydrogen peroxide reactions in skin: In vitro model relevant to study ROS reactions at inflammation

Eskandari, Mahboubeh; Rembiesa, Jadwiga; Startaitė, Lauryna; Holefors, Anna; Valančiūtė, Audronė; Faridbod, Farnoush; Ganjali, Mohammad Reza; Engblom, Johan; Ruzgas, Tautgirdas

doi:10.1016/j.aca.2019.05.032

Cited by 20 publications

(18 citation statements)

References 24 publications

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“…In a previous study, the possibility of SCOE to monitor the kinetics of polyphenol penetration through stratum corneum and its engagement in antioxidative reactions in the presence of H 2 O 2 in skin has been demonstrated [25]. SCOE allowed us to assess permeability and engagement of CA in antioxidant reactions, which are catalyzed by peroxidase like enzymes present in skin.…”

Section: Ex-vivo Evaluation Of Permeation and Role Of Ca In Antioxidamentioning

confidence: 99%

“…The antioxidant effect exerted by CA-ETHO as well as CA penetration were more intense with respect to the drug in solution, demonstrating that CA delivery through the skin should be ascribed to the ETHO supramolecular structure and not merely to the ethanol presence. The integrity of skin membranes was assessed by electrical impedance measurements of their resistance [25]. The measured values were higher than 3 kΩ, demonstrating that skin membranes have good integrity, i.e., intact stratum corneum.…”

Section: Ex-vivo Evaluation Of Permeation and Role Of Ca In Antioxidamentioning

confidence: 99%

“…Finally, an amperometric study has been conducted in order to evaluate CA permeation through the skin. Particularly, an in vitro tool based on skin covered oxygen electrode (SCOE), has been exploited to assess the kinetics of CA penetration through stratum corneum and its involvement in antioxidative reactions in skin at the presence of hydrogen peroxide, H 2 O 2 [25]. In the SCOE apparatus, an electrode allows registration of O 2 concentration changes in the membrane due to reactions of H 2 O 2 and polyphenols in the skin [26].…”

Section: Introductionmentioning

confidence: 99%

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Design and Characterization of Ethosomes for Transdermal Delivery of Caffeic Acid

et al. 2020

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The present investigation describes a formulative study aimed at designing ethosomes for caffeic acid transdermal administration. Since caffeic acid is characterized by antioxidant potential but also high instability, its encapsulation appears to be an interesting strategy. Ethosomes were produced by adding water into a phosphatidylcholine ethanol solution under magnetic stirring. Size distribution and morphology of ethosome were investigated by photon correlation spectroscopy, small-angle X-ray spectroscopy, and cryogenic transmission electron microscopy, while the entrapment capacity of caffeic acid was evaluated by high-performance liquid chromatography. Caffeic acid stability in ethosome was compared to the stability of the molecule in water, determined by mass spectrometry. Ethosome dispersion was thickened by poloxamer 407, obtaining an ethosomal gel that was characterized for rheological behavior and deformability. Caffeic acid diffusion kinetics were determined by Franz cells, while its penetration through skin, as well as its antioxidant activity, were evaluated using a porcine skin membrane–covered biosensor based on oxygen electrode. Ethosome mean diameter was ≈200 nm and almost stable within three months. The entrapment of caffeic acid in ethosome dramatically prolonged drug stability with respect to the aqueous solution, being 77% w/w in ethosome after six months, while in water, an almost complete degradation occurred within one month. The addition of poloxamer slightly modified vesicle structure and size, while it decreased the vesicle deformability. Caffeic acid diffusion coefficients from ethosome and ethosome gel were, respectively, 137- and 33-fold lower with respect to the aqueous solution. At last, the caffeic acid permeation and antioxidant power of ethosome were more intense with respect to the simple solution.

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Section: Ex-vivo Evaluation Of Permeation and Role Of Ca In Antioxidamentioning

confidence: 99%

Section: Ex-vivo Evaluation Of Permeation and Role Of Ca In Antioxidamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Design and Characterization of Ethosomes for Transdermal Delivery of Caffeic Acid

et al. 2020

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“…The inhibition process was confirmed by using skin membranes firmly attached to oxygen electrodes. The set-up is known as skin-covered oxygen electrode (SCOE), which measures O 2 liberated by skin catalase when the electrode is exposed to a solution of H 2 O 2 [25,28,37]. Figure S1 in the supporting information provides an example of the current response when the SCOE is exposed to H 2 O 2 in the absence and in the presence of a catalase inhibitor, specifically sodium azide, NaN 3 .…”

Section: Inhibition Of Catalase Activity In Skinmentioning

confidence: 99%

Visualisation of H2O2 penetration through skin indicates importance to develop pathway-specific epidermal sensing

et al. 2020

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Elevated amounts of reactive oxygen species (ROS) including hydrogen peroxide (H2O2) are observed in the epidermis in different skin disorders. Thus, epidermal sensing of H2O2 should be useful to monitor the progression of skin pathologies. We have evaluated epidermal sensing of H2O2 in vitro, by visualising H2O2 permeation through the skin. Skin membranes were mounted in Franz cells, and a suspension of Prussian white microparticles was deposited on the stratum corneum face of the skin. Upon H2O2 permeation, Prussian white was oxidised to Prussian blue, resulting in a pattern of blue dots. Comparison of skin surface images with the dot patterns revealed that about 74% of the blue dots were associated with hair shafts. The degree of the Prussian white to Prussian blue conversion strongly correlated with the reciprocal resistance of the skin membranes. Together, the results demonstrate that hair follicles are the major pathways of H2O2 transdermal penetration. The study recommends that the development of H2O2 monitoring on skin should aim for pathway-specific epidermal sensing, allowing micrometre resolution to detect and quantify this ROS biomarker at hair follicles.Graphical abstract

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“…Antioxidants are often used to protect and maintain the skin’s normal function, but studies to assess their effects can be costly and time-consuming. Eskandari et al 1 developed a system for monitoring H 2 O 2 and antioxidant reactions in the skin by employing a skin membrane-covered oxygen electrode (SCOE). This system generates amperometric responses to H 2 O 2 concentrations down to 0.05 mM and permits the study of polyphenol penetration and reaction with H 2 O 2 in the skin.…”

Section: System For the Detection Of Antioxidant Activity In The Skinmentioning

confidence: 99%

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Polyphenol-hydrogen peroxide reactions in skin: In vitro model relevant to study ROS reactions at inflammation

Cited by 20 publications

References 24 publications

Design and Characterization of Ethosomes for Transdermal Delivery of Caffeic Acid

Design and Characterization of Ethosomes for Transdermal Delivery of Caffeic Acid

Visualisation of H2O2 penetration through skin indicates importance to develop pathway-specific epidermal sensing

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