Polyphenol-enriched fraction and the compounds isolated from Garcinia indica fruits ameliorate obesity through suppression of digestive enzymes and oxidative stress

Munjal, Kavita; Ahmad, Sheeraz; Gupta, Apeksha; Haye, Abdul; Amin, Saima; Mir, Showkat R.

doi:10.4103/pm.pm_587_19

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…In continuation to our studies related to the preparative isolation of therapeutically important phytoconstituents [29, 30], this study describes the pilot‐scale isolation of Taxol and 10‐DAB III (Figure 1). Taxol is a diterpenic pseudo‐alkaloid that acts as a potent anti‐proliferative therapeutics.…”

Section: Resultsmentioning

confidence: 95%

Purification and characterization of Taxol and 10‐Deacetyl baccatin III from the bark, needles, and endophytes of Taxus baccata by preparative high‐performance liquid chromatography, ultra‐high‐performance liquid chromatography‐mass spectrometry, and nuclear magnetic resonance

Nurullah

Usmani

Ahmad

et al. 2023

J of Separation Science

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Taxol and 10‐Deacetyl baccatin III are major taxanes in the bark, needles, and endophytes of Taxus baccata. The current study aimed to develop a process for their separation from different matrices. Crude taxoid was prepared by extraction of samples with methanol, followed by partitioning with dichloromethane and precipitation with hexane. Analytical high‐performance liquid chromatography involved isocratic elution on C18 column (4.6 × 250 mm, 5 μm) with methanol‐water (70:30 v/v) at a flow rate of 1 ml/min. Injection volume was 20 μl and detection was carried out at 227 nm. The content of Taxol and 10‐Deacetyl baccatin III in bark, needles and endophytic culture broth was 11.19 and 1.75 μg/mg; 11.19 and 1.75 μg/mg; and 2.80 and 0.22 μg/L, respectively. Preparative high‐performance liquid chromatography was done on C18 column (10 × 250 mm, 5 μm) at a flow rate of 10 ml/min. About 20 g crude taxoid was processed in < 3 h with a recovery of about 90% for both the analytes. The purity of recovered Taxol and 10‐Deacetyl baccatin III determined by ultra‐high‐performance liquid chromatography‐mass spectrometry was found to be 95.78 ± 3.63% and 99.72 ± 0.18%, respectively. The structure of recovered Taxol was confirmed by nuclear magnetic resonance. The method can find use in biotransformation studies.

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Section: Resultsmentioning

confidence: 95%

Purification and characterization of Taxol and 10‐Deacetyl baccatin III from the bark, needles, and endophytes of Taxus baccata by preparative high‐performance liquid chromatography, ultra‐high‐performance liquid chromatography‐mass spectrometry, and nuclear magnetic resonance

Nurullah

Usmani

Ahmad

et al. 2023

J of Separation Science

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“…The IC 50 value for alpha‐amylase inhibition for G. indica rind powder was 58.43 μg/mL. Similarly, 367.4 (μg/mL) alpha‐amylase inhibition value observed in methanol extract G. indica rind (Munjal et al ., 2020) and 34.56 (μg/mL) ethanol 70% G.cowa rind powder (Wanna et al ., 2023). Kumar et al .…”

Section: Resultsmentioning

confidence: 99%

“…Table 3 represent the phytochemical analysis of G. indica rind powder. TPC was measured at 28.54 mg GAE/g, closely approximating the value reported for fully ripe G. indica which was 22.07 mg GAE/g (Munjal et al ., 2020). However, this value was lower than that observed in G. fusca and G. tinctoria , which recorded values of 35.17 mg GAE/g and 70.84 mg GAE/g, respectively.…”

Section: Resultsmentioning

confidence: 99%

Exploring the properties of Garcinia indica Linn rind powder and its possible usage in food industry

Awari,

Kumar,

Kaushik

et al. 2024

Int J of Food Sci Tech

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SummaryThe study investigates, the potential utilisation of the Garcinia indica linn rind powder as a valuable and healthy ingredient for food industry. Techno‐functional properties revealed the functional attributes which is crucial for product development. Characterisation of the powder and extract revealed the physical, chemical, and structural properties through FTIR, HPLC, and SEM. Therefore, moisture content (5.45%), ash content (2.80%), fibre content (7.56%), and fat content (9.54%) were reported in G. indica linn rind. Phytochemical analysis indicated significant levels of phenolic and flavonoid compounds, suggesting antioxidant potential, supported by inhibition in the DPPH assay with IC50 value of 485.1 μg/mL. In‐vitro assays revealed inhibitory effects on α‐amylase with IC50 value of 58.43 μg/mL and glucose with an IC50 value of 0.39 μg/mL, indicating potential benefits in blood glucose management and weight control. Overall, G. indica rind powder shows promise as a natural ingredient with diverse applications in functional foods and nutraceuticals, offering potential health benefits through innovative product formulations.

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“…The amla and kokum (slice or powder) analyzed in the current study were found to display high α-amylase and α-glucosidase inhibitory activity even at lower dilutions of the extracts, thereby indicating their potent anti-hyperglycemic activity. In an earlier study, phenolic-rich fractions of kokum were found to have α-amylase inhibitory activity with IC 50 values ranging from 349.7 to 980.0 μg/mL [ 64 ]. In another study, extracts of amla at the highest concentration were found to have α-amylase and α-glucosidase inhibitory activity at 84.15% and 93.9% respectively [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemicals-linked food safety and human health protective benefits of the selected food-based botanicals

Christopher,

Shetty

2024

PLoS ONE

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Phytochemicals-rich food-based botanicals including traditional or under-utilized plant-based ingredients can serve a dual functional role to help counter food contamination of bacterial origin, while also addressing the rise of diet-linked non-communicable chronic diseases (NCDs) such as type 2 diabetes, chronic hypertension and the associated oxidative stress. Hence the screening of these food-based botanicals for their phenolic content and profile, as well as antimicrobial, antioxidant, anti-hyperglycemic and anti-hypertensive properties has relevant merit. Using in vitro assay models, hot water extracts of different forms (slice, pickle, or powder) of amla (Phyllanthus emblica), clove (Syzygium aromaticum), kokum (Garcinia indica), and garlic (Allium sativum) were analyzed for their total soluble phenolic content (TSP) and phenolic profile as well as antimicrobial activity against strains of Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli that are associated with food-borne disease outbreaks. In addition, the antioxidant, anti-hyperglycemic and anti-hypertensive activity of the extracts were also determined using in vitro assay models, with the goal of establishing a dual functional role of the food safety and health protective benefits of these botanicals. A high baseline TSP content was observed in all the extracts and the major phenolic phytochemicals detected were gallic, cinnamic, ellagic, benzoic, dihydroxybenzoic, protocatechuic, and p-coumaric acid along with catechin and rutin. All extracts displayed significant antimicrobial activity against most of the bacterial strains tested and the antimicrobial activity was specific for each strain targeted in this study. Furthermore, significant antioxidant, anti-hyperglycemic and antihypertensive activity were observed among the botanical extracts, especially among the amla and kokum extracts. These results indicate that phytochemicals enriched botanicals, including amla and kokum, can be integrated into modern-day food preservation and dietary support strategies aimed at improving the food safety and health protective benefits of the food matrix.

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Polyphenol-enriched fraction and the compounds isolated from Garcinia indica fruits ameliorate obesity through suppression of digestive enzymes and oxidative stress

Cited by 9 publications

References 24 publications

Purification and characterization of Taxol and 10‐Deacetyl baccatin III from the bark, needles, and endophytes of Taxus baccata by preparative high‐performance liquid chromatography, ultra‐high‐performance liquid chromatography‐mass spectrometry, and nuclear magnetic resonance

Purification and characterization of Taxol and 10‐Deacetyl baccatin III from the bark, needles, and endophytes of Taxus baccata by preparative high‐performance liquid chromatography, ultra‐high‐performance liquid chromatography‐mass spectrometry, and nuclear magnetic resonance

Exploring the properties of Garcinia indica Linn rind powder and its possible usage in food industry

Phytochemicals-linked food safety and human health protective benefits of the selected food-based botanicals

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