Polyphenol-enriched cocoa protects the diabetic retina from glial reaction through the sirtuin pathway

Duarte, Diego Andreazzi; Rosales, Mariana A. B.; Papadimitriou, Alexandros; Silva, Kamila C.; Amancio, Vitor Hugo O.; Mendonça, Jacqueline Nakau; Lopes, Norberto P.; Faria, José B. Lopes de; Faria, Jacqueline M. Lopes de

doi:10.1016/j.jnutbio.2014.09.003

Cited by 46 publications

(32 citation statements)

References 47 publications

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“…The immunofluorescence assays in rMC-1 were performed as previously published. 22 The cover glasses with fixed cells were incubated with the appropriate antibodies: anti-LC3 (1:10, Novus Biologicals, LLC, Littleton, CO, USA) and anti-p62/ SQTSM1 (1:100, Santa Cruz Biotechnology) overnight at 48C, and the appropriate secondary antibodies were applied for 1 hour at room temperature. The cover glasses were examined under a fluorescence microscope (Zeiss, Oberkochen, Germany).…”

Section: Immunofluorescence Assaysmentioning

confidence: 99%

Defective Autophagy in Diabetic Retinopathy

Faria

Duarte

Montemurro

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Section: Immunofluorescence Assaysmentioning

confidence: 99%

Defective Autophagy in Diabetic Retinopathy

Faria

Duarte

Montemurro

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Mice that received Co prior to LPS challenge showed an attenuation of the LPS‐induced reactive gliosis, which could be beneficial in the model used. The effect of Co on reactive gliosis has not been previously reported, but other polyphenols have attenuated systemic inflammation‐induced spatial memory impairment via inhibition of astrocyte activation (Chen, Zhang, et al., ), reduced GFAP expression in retinal glial cells in diabetic rats (Duarte et al., ), and decreased astrocyte activation in a similar model of LPS‐induced neuroinflammatory mouse model (Khan et al., ).…”

Section: Discussionmentioning

confidence: 99%

Neuromodulatory Activity of Dietary Phenolics Derived from Corchorus olitorius L.

Wagdy

Abdel‐Kader

El‐Khatib

et al. 2019

Journal of Food Science

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Dietary phenolics are known for their potent antioxidant and anti-inflammatory activities, making them promising candidates for protection against neuroinflammation and neurodegeneration. Hydroalcohol extract of Egyptian species of Corchorus olitorius L. (Co) leaves was investigated for its neuroprotective effects in a lipopolysaccharide-induced neuroinflammatory mouse model. Twenty five metabolites were characterized from the bioactive extract using highperformance liquid chromatography HPLC/PDA/HRESI/MS n , revealing 1,5-dicaffeoylquinic acid (Co11) as one of the major constituents (5.7%), which was isolated and its identity was confirmed by spectral data as first report. Co significantly protected microglia against H 2 O 2 -induced cytotoxicity and immunohistochemistry showed reduced expression of the astrocytic marker, glial fibrillary acidic protein, and the inflammatory marker, cyclooxygenase-2. These findings correlated with significant improvement of cognitive functions and reduction of LPS-induced neurodegeneration in Co-treated mice as revealed by histopathology. The current study shows promising effects of Co in limiting neurodegeneration and cognitive impairment caused by neuroinflammation and glial cell activation.Practical Application: Information presented here shed light on the promising effects of Corchorus olitorius (Co) for the modulation of neuroinflammatory pathways improving the neuroinflammation-related neurodegeneration and cognitive decline. This makes Co a promising candidate as a nutraceutical supplement to be used against neuroinflammation-related disorders.

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“…The characteristics of retinal neurodegeneration are apoptosis of neuro cells and dysfunction of glial cells-mainly Müller cells [29,50,60]. In microvascular disease of diabetic retinopathy, both inner and outer blood retinal barrier break down [61].…”

Section: Diabetic Retinopathymentioning

confidence: 99%

Deficient Autophagy Contributes to the Development of Diabetic Retinopathy

Faria¹,

Dátilo²

2020

The Eye and Foot in Diabetes

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Autophagy is a self-degradation process essential to maintain intracellular homeostasis and cell survival, controlling elimination of pathogens, damage to organelles, and nutrient recycling to generate energy. Alterations in autophagic flux have been reported in the mechanisms of several diseases such as neurodegenerative diseases, cancer, diabetes mellitus, and its associated complications. Diabetic retinopathy (DR) is a microvascular complication of diabetes, affecting nearly 30% of diabetic patients. Several pathways are triggered and repressed in the development of DR, and autophagy showed to be relevant in the pathogenesis of this devastating complication. In this chapter, autophagy's involvement in the development and progression of DR will be discussed, mainly in retinal pigmented epithelial cells and retinal microvascular endothelial cells, as well as in Müller cells-the more prominent retinal glial cell.

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Polyphenol-enriched cocoa protects the diabetic retina from glial reaction through the sirtuin pathway

Cited by 46 publications

References 47 publications

Defective Autophagy in Diabetic Retinopathy

Defective Autophagy in Diabetic Retinopathy

Neuromodulatory Activity of Dietary Phenolics Derived from Corchorus olitorius L.

Deficient Autophagy Contributes to the Development of Diabetic Retinopathy

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