Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent

Felföldi, Tamás; Székely, Anna J.; Gorál, Róbert; Barkács, Katalin; Scheirich, Gergely; András, Judit; Rácz, Anikó; Màrialigeti, Károly

doi:10.1016/j.biortech.2009.12.053

Cited by 106 publications

(70 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrolytic fermentative acidogenic activity in anaerobic bioreactors is likely controlled by strict anaerobic bacteria like Longilinea, Clostridium, and Proteiniphilum (Yamada et al 2007;Kohda et al 1997;Letowski et al 2001;Chen and Dong 2005;Larsen et al 2009) and facultative aerobic bacteria such as Corynebacterium (Kampfer et al 2009). In the aerobic sludge, the high abundances of Thiobacillus, Comamonas, Diaphorobacter, Ottowia, and Weeksella (Table S8), which reportedly degrade several phenol and SCN − compounds (Felföldi et al 2010;Beller et al 2006;Klankeo et al 2009;Yuste et al 2000), were in accordance with the significant removal of pollutants in the final effluent. As referred in Table S8, the significant presence of PAHdegrading bacteria like Comamonas (Goyal and Zylstra 1996) and Diaphorobacter (Klankeo et al 2009) indicated the removal of corresponding pollutants in aerobic sludge leading to detoxification of CWW (Zhang et al 2013).…”

Section: Discussionmentioning

confidence: 67%

“…2, although anaerobic treatment was not effective, the combined system was quite efficient for the removal of SCN − (98.2 %) and CN − (85.4 %) ( Table S1). Degradation of SCN − , CN − , and other nitrogen and sulfur heterocyclic organic compounds was responsible for the release of ammonium and sulfate (Felföldi et al 2010;Staib and Lant 2007;Vazquez et al 2006;Maranon et al 2008;Gai et al 2007). Oxidation of sulfides and thiosulfate (Shoji et al 2014) may have also contributed to the increased concentration of inorganic sulfate in the aerobic effluent.…”

Section: Resultsmentioning

confidence: 99%

“…In the aerobic process, SCN − degradation and ammonification of NHCs was coupled with increased concentration of ammonia-nitrogen in the final effluent (Staib and Lant 2007;Vazquez et al 2006;Felföldi et al 2010), while total nitrogen (TN) remained approximately constant throughout the process. The absence of nitrifying bacteria in the aerobic sludge (Table S5) might be due to insufficient HRT (72 h) or an inhibitory effect of toxic compounds .…”

Section: Discussionmentioning

confidence: 99%

“…Coking wastewater (CWW) generated during coke production has garnered wide attention due to its mixture of diverse organic contaminants that are hazardous to ecosystems and human health (Song et al 2013;Zhang et al 2013;Felföldi et al 2010;Zhao et al 2014;Hou et al 2015). CWW is generally treated by biological methods such as conventional activated sludge processes (Vazquez et al 2006;Maranon et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the toxicity of contaminants like cyanides (CN − ) and phenols (Kim et al 2008;Sharma et al 2012), aerobic activated sludge processes can effectively remove phenol Electronic supplementary material The online version of this article (doi:10.1007/s00253-016-7591-8) contains supplementary material, which is available to authorized users. derivatives, polyaromatic hydrocarbons (PAH), nitrogenous heterocyclic compounds (NHCs), thiocyanate (SCN − ), thiosulfate (Felföldi et al 2010;Vazquez et al 2006;Zhang et al 2013;Bai et al 2009;Sharma and Philip 2015;Shoji et al 2014), releasing sulfate (SO 4 2− ), and ammonia (NH 4 + ) into treated effluent (Staib and Lant 2007). However, aerobic processes require high energy input, generate large volumes of sludge, and do not adapt well to influent fluctuations .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The combined anaerobic-aerobic biosystem is assumed to consume less energy for the treatment of high strength industrial wastewater. In this study, pollutant removal performance and microbial diversity were assessed in a longterm (over 300 days) bench-scale sequential anaerobicaerobic bioreactor treating coking wastewater. Anaerobic treatment removed one third of the chemical oxygen demand (COD) and more than half of the phenols with hydraulic retention time (HRT) of 42 h, while the combined system with total HRT of 114 h removed 81.8, 85.6, 99.9, 98.2, and 85.4 % of COD, total organic carbon (TOC), total phenols, thiocyanate, and cyanide, respectively. Two-dimensional gas chromatography with time-of-flight mass spectrometry showed complete removal of phenol derivatives and nitrogenous heterocyclic compounds (NHCs) via the combined system, with the anaerobic process alone contributing 58.4 and 58.6 % removal on average, respectively. Microbial activity in the bioreactors was examined by 454 pyrosequencing of the bacterial, archaeal, and fungal communities. Proteobacteria (61.2-93.4 %), particularly Betaproteobacteria (34.4-70.1 %), was the dominant bacterial group. Ottowia (14.1-46.7 %), Soehngenia (3.0-8.2 %), and Corynebacterium (0.9-12.0 %), which are comprised of phenol-degrading and hydrolytic bacteria, were the most abundant genera in the anaerobic sludge, whereas Thiobacillus (6.6-43.6 %), Diaphorobacter (5.1-13.0 %), and Comamonas (0.2-11.1 %) were the major degraders of phenol, thiocyanate, and NHCs in the aerobic sludge. Despite the low density of fungi, phenol degrading oleaginous yeast Trichosporon was abundant in the aerobic sludge. This study demonstrated the feasibility and optimization of less energy intensive treatment and the potential association between abundant bacterial groups and biodegradation of key pollutants in coking wastewater.

show abstract

Section: Discussionmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment

Hodgson

Smith

Marchesi

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND This research is focused on the effect of temperature on the growth of active biofilms on polypropylene (PP) filter media in aerobic fixed biofilm reactors (FBR) for wastewater treatment. RESULTS High‐throughput sequencing was used to explore the composition and diversity of the microbial community of 14‐days‐old (starting phase) biofilms grown at 10, 20 and 30°C. Members of the classes Proteobacteria, Bacteroidetes, and Firmicutes were predominant in all the biofilm samples retrieved from PP‐FBRs. A total of 108 genera of bacteria were identified, with some of them present in all three reactors, including Trichococcus, Zoogloea, Aeromonas, Acidovorax, and Malikias, among others. Besides these shared populations, certain genera were abundantly found in individual biofilm samples, like Brevundimonas (17.1%), Chitinimonas (10.3%) and Roseateles (39.3%), at 10, 20, and 30°C, respectively. The metabolic capabilities of active microbial communities in PP‐FBRs were estimated by assessing the changes in different variables (BOD, DO, and pH) in the influent and effluent during operation. A noteworthy BOD removal (66.6%) was shown by PP‐FBRs operating at 30°C, compared with 20°C (28.3%) and 10°C (28.8%),consistent with the DO levels recorded in the effluents, highest at 30°C (70.5%), and decreasing with declining temperatures. Substantial wastewater treatment efficiencies were observed in the reactors at 30°C, attributable to the higher relative abundance and diversity of microbial biofilms. CONCLUSIONS The development of physiologically active biofilms in PP at all prevailing temperatures strongly suggests that the material is suitable to be employed in FBRs for wastewater treatment at different operational temperatures. © 2018 Society of Chemical Industry

show abstract

Genome‐resolved metagenomics of a bioremediation system for degradation of thiocyanate in mine water containing suspended solid tailings

et al. 2017

View full text Add to dashboard Cite

Thiocyanate (SCN−) is a toxic compound that forms when cyanide (CN−), used to recover gold, reacts with sulfur species. SCN−‐degrading microbial communities have been studied, using bioreactors fed synthetic wastewater. The inclusion of suspended solids in the form of mineral tailings, during the development of the acclimatized microbial consortium, led to the selection of an active planktonic microbial community. Preliminary analysis of the community composition revealed reduced microbial diversity relative to the laboratory‐based reactors operated without suspended solids. Despite minor upsets during the acclimation period, the SCN− degradation performance was largely unchanged under stable operating conditions. Here, we characterized the microbial community in the SCN− degrading bioreactor that included solid particulate tailings and determined how it differed from the biofilm‐based communities in solids‐free reactor systems inoculated from the same source. Genome‐based analysis revealed that the presence of solids decreased microbial diversity, selected for different strains, suppressed growth of thiobacilli inferred to be primarily responsible for SCN− degradation, and promoted growth of Trupera, an organism not detected in the reactors without solids. In the solids reactor community, heterotrophy and aerobic respiration represent the dominant metabolisms. Many organisms have genes for denitrification and sulfur oxidation, but only one Thiobacillus sp. in the solids reactor has SCN− degradation genes. The presence of the solids prevented floc and biofilm formation, leading to the observed reduced microbial diversity. Collectively the presence of the solids and lack of biofilm community may result in a process with reduced resilience to process perturbations, including fluctuations in the influent composition and pH. The results from this investigation have provided novel insights into the community composition of this industrially relevant community, giving potential for improved process control and operation through ongoing process monitoring.

show abstract

Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent

Cited by 106 publications

References 41 publications

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment

Genome‐resolved metagenomics of a bioremediation system for degradation of thiocyanate in mine water containing suspended solid tailings

Contact Info

Product

Resources

About