Polyphasic approaches to the identification of predominant polyphosphate-accumulating organisms in a laboratory-scale anaerobic/aerobic activated sludge system.

Onda, Shin; Hiraishi, Akira; Matsuo, Yoshitaka; Takii, Susumu

doi:10.2323/jgam.48.43

Cited by 23 publications

(17 citation statements)

References 36 publications

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“…The pH was 6.8 in the anaerobic tank, 7.6-8.2 in the aerobic tank. The concentration of PO 4 -P in the effluent was 0.2 mg/L and the total phosphorus content of the sludge was 8.4% (Onda et al, 2002).…”

Section: Methodsmentioning

confidence: 97%

“…Recently, Hesselmann et al (1999) and Crocetti et al (2000) reported that a bacterium closely related to Rhodocyclus, named Candidatus Accumulibacter phosphatis (Hesselmann et al, 1999), was responsible for P-removal by cloning of 16S rDNA and fluorescent in situ hybridization (FISH). In our previous report (Onda et al, 2002) this bacterium was also shown to be the dominant PAO in a laboratory-scale anaerobic/aerobic continuous flow reactor by using denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA, quinone profiling, and FISH. However, it has never been successfully isolated, so far as we know.…”

mentioning

confidence: 88%

“…In this experiment we tried to isolate and characterize new PAOs from the laboratory-scale reactor described above (Onda et al, 2002). For isolation, pyruvate-supplemented agar plates were used in this study, because it was reported that the addition of sodium pyruvate to agar media greatly increased the numbers of colonies on agar plates from some environmental samples (Sugitate and Morikawa, 1999).…”

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confidence: 99%

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Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium.

Onda¹,

Takii²

2002

J. Gen. Appl. Microbiol.

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Section: Methodsmentioning

confidence: 97%

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confidence: 88%

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confidence: 99%

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Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium.

Onda¹,

Takii²

2002

J. Gen. Appl. Microbiol.

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“…Fluorescence in situ hybridization (FISH) has shown Accumulibacter to be an abundant organism in laboratory-scale EBPR cultures with various carbon sources (Levantesi et al, 2002;Liu et al, 2001;Oehmen et al, 2004Oehmen et al, , 2005bOnda et al, 2002;Pijuan et al, 2004;Zeng et al, 2003a). Accumulibacter has also been reported in full-scale EBPR systems (Saunders et al, 2003;Zilles et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

et al. 2006

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In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid-and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96 % of Bacteria) from this bioreactor, while postfluorescence in situ hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-b-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to Defluvicoccus vanus (16 % of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary.

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“…Since one homolog type of quinone predominates in a single genus or species of microorganisms in general, the content of each quinone homolog in complex microbial communities may be interpreted as a direct reflection of the content of microbial taxa with different quinones. Taking this advantage, the quinone profiling method has been applied to characterize microbial community structure in various environments, such as sewage and activated sludge 4,[8][9][10]13,17,18,20) , aquatic sediments 6,24) , hot springs 11) , soil 2,7,15,16) and compost 12) . Although the universality of quinone profiles as tools for estimating microbial community changes over time and space has been realized, the available information about the quantitative relationship between quinones and microbial biomass or cell counts is still limited.…”

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Significance of Lipoquinones as Quantitative Biomarkers of Bacterial Populations in the Environment

et al. 2003

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The direct total count of bacteria and the concentration of isoprenoid quinones were measured in 82 samples from different aquatic and soil environments. The geometric mean of the total count (TC)/total quinine (TQ) ratios obtained with different environments ranged from 1.1 to 2.2´10 3 cells fmol -1 . When the concentration of the total respiratory quinone (TRQ), i.e., ubiquinones and menaquinones, were taken into account, the geometric mean of the TC/TRQ ratios fell into a range of 2.2 to 4.1´10 3 cells fmol -1 . A high positive correlation was noted between TC and TQ (r 2 =0.9864) or TRQ (r 2 =0.9990). Based on the relationship between TC and TRQ, 1 nmol of the total quinone was estimated to be equivalent to 2.5´10 9 cells of bacteria on average. These results indicate that the concentration of the respiratory quinones can be used as a good measure of bacterial counts and biomass in the environment.

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Polyphasic approaches to the identification of predominant polyphosphate-accumulating organisms in a laboratory-scale anaerobic/aerobic activated sludge system.

Cited by 23 publications

References 36 publications

Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium.

Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium.

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

Significance of Lipoquinones as Quantitative Biomarkers of Bacterial Populations in the Environment

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