Polyphasic Analyses of Methanogenic Archaeal Communities in Agricultural Biogas Plants

Nettmann, Edith; Bergmann, Ingo; Pramschüfer, S.; Mundt, K.; Plogsties, Vincent; Herrmann, Christiane; Klocke, Michael

doi:10.1128/aem.02564-10

Cited by 26 publications

(43 citation statements)

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“…However, relative qPCR quantities of hydA, hfsB, and mcrA coding gene fragment were about 100 times lower than FISH quantities. In agreement with the findings of Nettman et al [31], quantities of often single-copy-per-genome hydA, hfsB, and mcrA were underestimated by the multi-copy 16S rRNA operons as true for most microbial genomes.…”

Section: Microbial Characterization: Batch Bioh 2 Experimentssupporting

confidence: 87%

Ultrasonic pretreatment of palm oil mill effluent: Impact on biohydrogen production, bioelectricity generation, and underlying microbial communities

Leaño

Anceno

Babel

2012

International Journal of Hydrogen Energy

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Section: Microbial Characterization: Batch Bioh 2 Experimentssupporting

confidence: 87%

Ultrasonic pretreatment of palm oil mill effluent: Impact on biohydrogen production, bioelectricity generation, and underlying microbial communities

Leaño

Anceno

Babel

2012

International Journal of Hydrogen Energy

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“…Restriction fragment length polymorphism and qPCR were used to conclude that Methanomicrobiales was the dominant group among methanogen orders. The methanogen community structures in six German full-scale biogas plants were investigated with qPCR along with fluorescence in situ hybridization (FISH) [53]. The results showed that five of the six plants were dominated by hydrogenutilizing Methanomicrobiales and only one plant with a low ammonia concentration was dominated by acetateutilizing Methanosaetaceae.…”

Section: Methanogensmentioning

confidence: 99%

Design and use of group-specific primers and probes for real-time quantitative PCR

Lim

Shin

Lee

et al. 2011

Front. Environ. Sci. Eng. China

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Real-time quantitative polymerase chain reaction (qPCR) has gained popularity as a technique to detect and quantify a specific group of target microorganisms from various environmental samples including soil, water, sediments, and sludge. Although qPCR is a very useful technique for nucleic acid quantification, accurately quantifying the target microbial group strongly depends on the quality of the primer and probe used. Many aspects of conducting qPCR assays have become increasingly routine and automated; however, one of the most important aspects, designing and selecting primer and probe sets, is often a somewhat arcane process. In many cases, failed or non-specific amplification can be attributed to improperly designed primer-probe sets. This paper is intended to provide guidelines and general principles for designing group-specific primers and probes for qPCR assays. We demonstrate the effectiveness of these guidelines by reviewing the use of qPCR to study anaerobic processes and biologic nutrient removal processes. qPCR assays using group-specific primers and probes designed with this method, have been used to successfully quantify 16S ribosomal Ribonucleic Acid (16S rRNA) gene copy numbers from target methanogenic and ammoniaoxidizing bacteria in various laboratory-and full-scale biologic processes. Researchers with a good command of primer and probe design can use qPCR as a valuable tool to study biodiversity and to develop more efficient control strategies for biologic processes.

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“…At the same time, while Methanosarcina thrive in environments with high acetate concentration; lower acetate concentrations benefit the dominance of Methanosaeta owing to their high affinity for acetate [12,[46][47][48]. As aforementioned, an enhanced bioproduction of acetic acid was reported in the pretreated sludge [4], the revealed shift in the methanogenic community could therefore be interpreted as an ecological consequence [30,49,50] of the sludge pretreatment.…”

Section: A C D E Bmentioning

confidence: 99%

Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

2016

Environmental Engineering and Activated Sludge Processes

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Background: Understanding the effects of pretreatment on anaerobic digestion of sludge waste from wastewater treatment plants is becoming increasingly important, as impetus moves towards the utilization of sludge for renewable energy production. Although the field of sludge pretreatment has progressed significantly over the past decade, critical questions concerning the underlying microbial interactions remain unanswered. In this study, a metagenomic approach was adopted to investigate the microbial composition and gene content contributing to enhanced biogas production from sludge subjected to a novel pretreatment method (maintaining pH at 10 for 8 days) compared to other documented methods (ultrasonic, thermal and thermal-alkaline).

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Polyphasic Analyses of Methanogenic Archaeal Communities in Agricultural Biogas Plants

Cited by 26 publications

References 0 publications

Ultrasonic pretreatment of palm oil mill effluent: Impact on biohydrogen production, bioelectricity generation, and underlying microbial communities

Ultrasonic pretreatment of palm oil mill effluent: Impact on biohydrogen production, bioelectricity generation, and underlying microbial communities

Design and use of group-specific primers and probes for real-time quantitative PCR

Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

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