Polypeptides in Tipula iridescent virus (TIV) and in TIV-infected hemocytes of Galleria mellonella (L.) larvae

Krell, Peter J.; Lee, Peter E.

doi:10.1016/0042-6822(74)90327-4

Cited by 22 publications

(5 citation statements)

References 25 publications

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“…In preliminary experiments, we have failed to detect glycosylation of structural or nonstructural proteins in FV3 infected cells (R. Goorha and A. Granoff, unpublished observation). A similar situation is found with several iridescent ICDVs (Wagner et al, 1974;Krell and Lee, 1974). Similarly, the presence of glycoproteins could not be detected in purified FV3 virions.…”

Section: 14d Posttranslational Modification Of Viral Proteinssupporting

confidence: 65%

“…Polyacrylamide gel analysis of mosquito iridescent virus disclosed nine proteins, none of which reacted with Schiffs reagent, consistent with a lack of glycoproteins in these virions (Wagner et al, 1974). Tipula iridescent virus was found to contain 28 polypeptides ranging in molecular weight from 17.5 to 300 x 10 3 ; none was a glycoprotein (Krell and Lee, 1974). In keeping with their small size, plant ICDVs have a simple electrophoretic profile of protein; cauliflower mosaic virus contains only two structural proteins, having molecular weights of 68,000 and 33,000 (Tezuka and Taniguchi, 1972).…”

Section: Proteinsmentioning

confidence: 86%

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Icosahedral Cytoplasmic Deoxyriboviruses

Goorha

Granoff

1979

Comprehensive Virology

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Section: 14d Posttranslational Modification Of Viral Proteinssupporting

confidence: 65%

Section: Proteinsmentioning

confidence: 86%

Icosahedral Cytoplasmic Deoxyriboviruses

Goorha

Granoff

1979

Comprehensive Virology

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“…The abundance of such proteins in vaccinia virus suggests that virus membrane and core morphogenesis is ascribable to self assembly (Ichihashi et cd, 1984). Different polypeptide patterns have been published for members of the invertebrate iridovirus group (Kelly and Tinsley, 1972;Krell and Lee, 1974;Wagner et aZ., 1974 In a previous study (Barray and Devauchelle, 1979), it was shown that after solubilization of CIV with SDS-@-ME, 16 polypeptides were resolved with molecular weights ranging from 7K to 120K, with a major polypeptide of 51K. This major polypeptide represents about 40% of the 129 total viral protein.…”

Section: Viral Dna-binding Proteinsmentioning

confidence: 98%

Characterization and localization of CIV polypeptides

Cérutti

Devauchelle

1985

Virology

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In order to detect the structural proteins linked with disulfide bonds, CIV was solubilized and electrophoresed under nonreducing conditions in the first dimension and then under reducing conditions in the second dimension. The viral polypeptides linked originally with disulfide bonds were separated into subunits. The complexes were trimers (P'50) or dimers (P60 and P10). The apparent molecular weights of P81, P53, and P49 changed significantly according to the composition of the lysis buffer used, suggesting that the differences in their molecular weights were due to conformational changes produced by reduction of their intramolecular disulfide bonds. Sulfhydryl-containing polypeptides (P'50-P50, P60, P100, and P33) were detected by N-[14C]ethylmaleimide, and the accessibility of these residues was analyzed after successive stripping of the CIV particle. Radioiodination of external polypeptides by [125I]iodosulfanilic acid shows only one intensively labeled spot corresponding to the P50 polypeptide, whereas P'50 was only slightly labeled. Six viral polypeptides P81, P60, P31, P17, P13, and P10 were revealed to possess high affinity for CIV DNA. A structural model of CIV is proposed and discussed.

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“…As shown (Stoltz, 1971, Wagner et al, 1973, chloriridoviruses have bigger sizes and contain fewer polypeptides in comparison with other iridoviruses of insects. Iridovirus from Tipula paludosa contains 28 polypeptides with molecular weight varying from 17.5 kDa to 30 kDa (Krell and Lee, 1974). Iridovirus from Sericestis pruinosa contains 22 polypeptides (Elliot, Lescott and Kelly, 1977), iridoviruses from Simulium and Chilo 25 polypeptides (Devauchelle et al, 1985).…”

Section: Organic Solventsmentioning

confidence: 99%

Some biological properties of сhloriridovirus from Culiseta mosquitoes (Diptera: Culicidae)

Buchatsky,

Filenko,

Sutugina

2024

J. Vet. Med. Biotechnol. Biosafety

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Two strains of mosquito iridescent viruses (Chloriridovirus) were isolated in Ukraine from seek larvae of bloodsucking mosquitoes Culiseta annulata (Schrank, 1776) and Culiseta morsitans (Theobald, 1901). Electron microscopic study of tissues from these larvae revealed icosahedral virus particles ranging from 180 nm to 5 nm in diameter, containing dense, pleomorphic nucleotides. Viruses were assembled in the cytoplasm within spherical virosomes. Both viruses contained DNA and 11 polypeptides with molecular weights varying from 16 kD to 98 kD. A DNA restriction analysis of both strains of mosquito chloriridovirus of the genus Culiseta and cloning of fragments of their genomes by genetic engineering methods was performed. Isolated strains were sensitive to ultraviolet insolation and heating and were stable to organic solvents such as ether and chloroform. Both isolated strains grew well in mosquito (Aedes aegypti, Aedes pseudoscutellaris, Aedes albopictus) and in Lepidoptera (Euxoa scandens, Antheraea pernyi) cell lines. A close antigenic relationship has been found between isolated strains and Chloriridovirus from Aedes cantans. Some antigenic relationship was also demonstrated between isolated strains and still unclassified iridovirus from carp (Cyprinus carpio). These findings imply that both strains share some similarity in structural and biochemical characteristics and belong to the Chloriridovirus genus of the Iridoviridae family

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Polypeptides in Tipula iridescent virus (TIV) and in TIV-infected hemocytes of Galleria mellonella (L.) larvae

Cited by 22 publications

References 25 publications

Icosahedral Cytoplasmic Deoxyriboviruses

Icosahedral Cytoplasmic Deoxyriboviruses

Characterization and localization of CIV polypeptides

Some biological properties of сhloriridovirus from Culiseta mosquitoes (Diptera: Culicidae)

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