Polypeptide Materials: New synthetic methods and applications

Deming, Timothy J.

doi:10.1002/adma.19970090404

Cited by 206 publications

(190 citation statements)

References 64 publications

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“…Better results were obtained than when the cDNA genes were used, although the levels of protein production remained relatively low, especially for high molecular weight variants. 18 The best results reported thus far with E. coli expression systems were obtained by Winkler et al for spider dragline silk (25-40 mg L 21 cell culture) 19 and Krejchi et al for an (AG) 3 EG 20 repetitive material. 21,22 To increase protein yield, high cell density production was performed, and allowed preparation of multigram quantities of product (25 g protein/35 L culture).…”

Section: Spider Dragline Silkmentioning

confidence: 70%

“…An important challenge, however, is to translate these concepts into synthetic or bio-inspired materials, which would lead to new kinds of high performance materials. 3 Because of the importance of control at the monomer level, the most promising approach to this target is to use the biosynthetic pathways of (micro)-organisms to synthesize macromolecular materials.…”

Section: Introductionmentioning

confidence: 99%

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Protein-based materials, toward a new level of structural control

2001

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Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials. In many respects, nature's intricately designed products provide challenging examples for materials scientists, but translation of natural structural concepts into bio-inspired materials requires a level of control of macromolecular architecture far higher than that afforded by conventional polymerization processes. An increasingly important approach to this problem has been to use biological systems for production of materials. Through protein engineering, artificial genes can be developed that encode protein-based materials with desired features. Structural elements found in nature, such as b-sheets and a-helices, can be combined with great flexibility, and can be outfitted with functional elements such as cell binding sites or enzymatic domains. The possibility of incorporating non-natural amino acids increases the versatility of protein engineering still

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Section: Spider Dragline Silkmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Protein-based materials, toward a new level of structural control

2001

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“…1 However, in contrast to the well-studied situation of flexible block copolymer systems, morphologies resulting from the self-assembly of semiflexible and rod-like block copolymers are relatively less understood. For instance, experiments on rod-coil block copolymers have suggested rich morphological phase behavior which includes layered smectics, arrowheads, wavy lamellae, zigzags, etc.…”

Section: Introductionmentioning

confidence: 99%

Communication: Self-assembly of semiflexible-flexible block copolymers

Kumar

Ganesan

2012

The Journal of Chemical Physics

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We apply the methodology of self-consistent Brownian dynamics simulations to study the selfassembly behavior in melts of semiflexible-flexible diblock copolymers as a function of the persistence length of the semiflexible block. Our results reveal a novel progression of morphologies in transitioning from the case of flexible-coil to rod-coil copolymers. At even moderate persistence lengths, the morphologies in the semiflexible-block rich region of the phase diagram transform to liquid crystalline phases. In contrast, the phases in the flexible-block rich region of the phase diagram persist up to much larger persistence lengths. Our analysis suggests that the development of orientational order in the semiflexible block to be a critical factor influencing the morphologies of self-assembly. Introduction. Recently, the self-assembly characteristics of block copolymers in which one or more of the blocks possess appreciable degree of stiffness has attracted attention in many applications.1 However, in contrast to the well-studied situation of flexible block copolymer systems, morphologies resulting from the self-assembly of semiflexible and rod-like block copolymers are relatively less understood. For instance, experiments on rod-coil block copolymers have suggested rich morphological phase behavior which includes layered smectics, arrowheads, wavy lamellae, zigzags, etc.2 An understanding of the dependencies of such morphologies upon the physicochemical characteristics of the polymers is essential for achieving fruition in the desired applications.There have been some attempts to model the selfassembly behavior of block copolymers containing semiflexible and rigid blocks. Early researches in this context used scaling arguments and/or analytical theories to clarify the self-assembly characteristics for the case in which one of the blocks was "rigid" or rod-like. 3,4 More recently, a number of researchers have also extended the numerical framework of polymer self-consistent field theory (SCFT) (Ref. 5) to address the self-assembly characteristics of such rod-coil block copolymers. 6,7 Despite such advances, modeling the self-assembly behavior of di-and multiblock copolymers containing semiflexible units remain an outstanding challenge. Specifically, in such a context, the mathematical framework of polymer SCFT requires the solution of a diffusion-like equation in the space of two-dimensional orientational vector in addition to the physical space dimensions.

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“…[36][37][38][39] Bearing multifunctional building blocks and labile structural functionalities, such biopolymers have greatly benefited from click chemistry.…”

Section: Peptidesmentioning

confidence: 99%

Click Chemistry: A Powerful Tool to Create Polymer‐Based Macromolecular Chimeras

Droumaguet

Velonia

2008

Macromol. Rapid Commun.

174

111

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The combination of polymeric with biological materials, to create biohybrid macromolecules that merge the properties of both the natural and synthetic components, is a flourishing area in both life sciences and biotechnology. The click chemistry philosophy has recently provided a powerful tool in this direction, leading to a plethora of novel, tailor‐made biomacromolecules with unprecedented structural characteristics and properties. The different synthetic strategies, using the alkyne–azide click cycloadditions to bioorthogonally achieve the coupling of synthetic polymers with nucleic acids, peptides, sugars, proteins or even viruses and cells is described. The review covers the latest developments in this very dynamic and rapidly expanding field.magnified image

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Polypeptide Materials: New synthetic methods and applications

Cited by 206 publications

References 64 publications

Protein-based materials, toward a new level of structural control

Protein-based materials, toward a new level of structural control

Communication: Self-assembly of semiflexible-flexible block copolymers

Click Chemistry: A Powerful Tool to Create Polymer‐Based Macromolecular Chimeras

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