Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma

Alaiya, Ayodele; Roblick, Uwe J.; Egevad, Lars; Carlsson, Adelaide; Franzén, Bo; Volz, Daniela; Huwendiek, Sören; Linder, Stig; Auer, Gert

doi:10.1155/2000/351963

Cited by 78 publications

(52 citation statements)

References 15 publications

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“…According to a recent study by Hellman et al (2003), there seem to be two types of vaginal carcinoma with age-related aetiology: one type occurring at younger age with aetiological factors similar to cervical carcinoma and another type occurring at older age with different aetiology. This might explain why some vaginal and cervical carcinomas seem to be more homogeneous in their gene expression, whereas others are more heterogeneous.Previous studies have described marked variations in the expression of cell cycle-related proteins, stress proteins and members of cytoskeletal proteins between benign and malignant epithelial tumours of lung, breast, ovary and prostate gel-separated proteins (Alaiya et al, 2000a;Bergman et al, 2000). Similarly, in this study, we observed high expression of HSP 27, GST and Apolipoprotein A1 in both cervical and vaginal cancers compared with normal vaginal tissue.…”

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confidence: 84%

Protein expression patterns in primary carcinoma of the vagina

Hellman

Schedvins

et al. 2004

Br J Cancer

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Protein patterns in six samples from primary vaginal cancers, in five from normal vaginal tissue and in five primary cervical cancers, were analysed using two-dimensional polyacrylamide gel electrophoresis (2-DE). Protein expression profile was evaluated by computer-assisted image analysis (PDQUEST) and proteins were subsequently identified using matrix-assisted laser desorption/ ionisation mass spectrometry. The aim was to analyse the protein expression profiles using the hierarchical clustering method in vaginal carcinoma and to compare them with the protein pattern in cervical carcinoma in order to find a helpful tool for correct classification and for increased biomedical knowledge. Protein expression data of a distinct set of 33 protein spots were differentially expressed. These differences were statistically significant (Mann -Whitney signed-Ranked Test, Po0.05) between normal tissue, vaginal and cervical cancer. Furthermore, protein profiles of pairs of primary vaginal and cervical cancers were found to be very similar. Some of the protein spots that have so far been identified include Tropomyosin 1, cytokeratin 5, 15 and 17, Apolipoprotein A1, Annexin V, Glutathione-S-transferase. Others are the stress-related proteins, calreticulin, HSP 27 and HSP 70. We conclude that cluster analysis of proteomics data allows accurate discrimination between normal vaginal mucosa, primary vaginal and primary cervical cancer. However, vaginal and cervical carcinomas also appear to be relatively homogeneous in their gene expression, indicating similar carcinogenic pathways. There might, further, be a possibility to identify tumour-specific markers among the proteins that are differentially expressed. The results from this study have to be confirmed by more comprehensive studies in the future. Primary carcinoma of the vagina (PCV) is a rare disease affecting predominantly postmenopausal women (Pecorelli, 2001). Histologically, the majority of PCV consist of squamous cell carcinomas (Pecorelli, 2001). Owing to the rarity of this disease, little is known about the aetiological and prognostic factors. Like cervical carcinomas, PCV has been shown to be associated with HPV, but only in about 50% of the cases (Daling and Sherman, 1992;Hildesheim et al (1997)). The prognosis for PCV is quite poor with an overall 5-year survival rate of about 50%, which is worse than for cervical carcinoma (Pecorelli, 2001). Early detection is crucial for the prognosis.It has been suggested that vaginal and cervical carcinomas have common aetiology since vaginal tumours often occur as second primary malignancy in patients with a history of cervical dysplasia and/or neoplasia or hysterectomy due to these disorders (Choo and Anderson, 1982;Benedet et al, 1983;Brinton et al, 1990;Eddy et al, 1991;Kirkbride et al, 1995). In the clinical situation, it is sometimes difficult to discriminate between cervical and vaginal carcinomas, especially in patients with prior cervical disease. As 95% of the recurrences of cervical carcinoma occur within 5 years, ma...

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supporting

confidence: 84%

Protein expression patterns in primary carcinoma of the vagina

Hellman

Schedvins

et al. 2004

Br J Cancer

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“…27 This study also reported lower amounts of tropomyosin-1 and -2 and cytokeratin 18 in prostate cancer than in BPH. The same group reported in a subsequent study that 23 proteins out of 800 were somewhat different in amount between BPH and prostate cancer specimens.…”

Section: Proteomic Approaches For Prostate Cancersupporting

confidence: 57%

Proteome analysis of prostate cancer

Kuruma

Egawa

Ohishi

et al. 2004

Prostate Cancer Prostatic Dis

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In this paper, we briefly review cancer proteomics in general, with particular attention to our proteome analyses of prostate cancer. Our efforts include development of new tools and novel approaches to discovering proteins potentially useful as cancer diagnostic and/or prognostic biomarkers or as therapeutic targets. To this end, we analyzed prostate cancer proteomes using twodimensional gel electrophoresis employing agarose gels for the initial isoelectric focusing step (agarose 2-DE), with mass spectrometry used for protein identification. Agarose 2-DE offers advantages over the more widely used immobilized pH gradient 2-DE for separating high molecular mass proteins (15-500 kDa), thereby increasing its power to detect changes in the cancer's high-molecular mass proteomes.

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“…It is an important molecular chaperone involved in 'quality control' within secretory pathways and has important regulatory functions such as modulation of steroid hormone receptors, retinoic acid receptors and regulation of cell adhesion. Increased levels of calreticulin have been reported in hepatocellular, colon, prostate and bladder carcinomas and as well as in radioresistant squamous carcinoma (Ramsamooj et al, 1995;Alaiya et al, 2000;Yoon et al, 2000;Brunagel et al, 2003;Iwaki et al, 2004).…”

Section: Antioxidation and Detoxificationmentioning

confidence: 99%

Differential tissue-specific protein markers of vaginal carcinoma

et al. 2009

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The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin -proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.

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Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma

Cited by 78 publications

References 15 publications

Protein expression patterns in primary carcinoma of the vagina

Protein expression patterns in primary carcinoma of the vagina

Proteome analysis of prostate cancer

Differential tissue-specific protein markers of vaginal carcinoma

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