Polypeptide-engineered physical hydrogels designed from the coiled-coil region of cartilage oligomeric matrix protein for three-dimensional cell culture

Yao, M.; Yang, Jie; Du, Ming-Shuo; Song, Ji-Tao; Yu, Yong; Chen, Wei; Zhao, Yuan‐Di; Liu, Bo

doi:10.1039/c4tb00107a

Cited by 21 publications

(24 citation statements)

References 44 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In that approach after injection and in situ hardening, the gel is gradually degraded to provide new volume for neo-tissue formation and replacement by the patient’s own tissue [2]. Natural hydrogels like collagen, chitosan, and alginate as well as synthetic polyethylene glycol (PEG) and polypeptide gels are used as a carrier in stem cell delivery in regenerative medicine [6–13]. Neural stem cells (NSCs) encapsulated in alginate gels differentiated into neuronal lineages only in gels with an elastic modulus similar to that of brain tissue (100–1000 Pa) [14].…”

Section: Introductionmentioning

confidence: 99%

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Moeinzadeh

Jabbari

2015

European Polymer Journal

View full text Add to dashboard Cite

Due to their high water content and diffusivity of nutrients and biomolecules, hydrogels are very attractive as a matrix for growth factor immobilization and in situ delivery of cells to the site of regeneration in tissue engineering. The formation of micellar structures at the nanoscale in hydrogels alters the spatial distribution of the reactive groups and affects the rate and extent of crosslinking and mechanical properties of the hydrogel. Further, the degradation rate of a hydrogel is strongly affected by the proximity of water molecules to the hydrolytically degradable segments at the nanoscale. The objective of this review is to summarize the unique properties of micellar hydrogels with a focus on our previous work on star polyethylene glycol (PEG) macromonomers chain extended with short aliphatic hydroxy acid (HA) segments (SPEXA hydrogels). Micellar SPEXA hydrogels have faster gelation rates and higher compressive moduli compared to their non-micellar counterpart. Owing to their micellar structure, SPEXA hydrogels have a wide range of degradation rates from a few days to many months as opposed to non-degradable PEG gels while both gels possess similar water contents. Furthermore, the viability and differentiation of mesenchymal stem cells (MSCs) is enhanced when the cells are encapsulated in degradable micellar SPEXA gels compared with those cells encapsulated in non-micellar PEG gels.

show abstract

Section: Introductionmentioning

confidence: 99%

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Moeinzadeh

Jabbari

2015

European Polymer Journal

View full text Add to dashboard Cite

show abstract

“…Hydrogel materials offer a wealth of potential applications (Elisseeff 2008), in that the biological, chemical, and mechanical properties may be tailored for particular applications through modification of the structure of the polymer and cross-linker. They have innate capacity to absorb and retain large volumes of water relative to the mass of the hydrogel itself (Elisseeff 2008;Xu and Kopeček 2008;Yao et al 2014). Moreover, hydrogels are often highly biocompatible due to their capacity for water absorption, physical elasticity, and relative softness (Kopeček 2007, Yao et al 2014.…”

Section: Coiled-coil Hydrogelsmentioning

confidence: 99%

“…They have innate capacity to absorb and retain large volumes of water relative to the mass of the hydrogel itself (Elisseeff 2008;Xu and Kopeček 2008;Yao et al 2014). Moreover, hydrogels are often highly biocompatible due to their capacity for water absorption, physical elasticity, and relative softness (Kopeček 2007, Yao et al 2014. The aforementioned qualities make hydrogels a convincing substitute for native extracellular matrix (Slaughter et al 2009;Yao et al 2014), as well as suitable candidates for cell culture applications (Frampton et al 2011;Jung et al 2011), biomedical uses such as wound treatment (Wong et al 2011;Lin et al 2011), and drug delivery (Garbern et al 2011;Guziewicz et al 2011).…”

Section: Coiled-coil Hydrogelsmentioning

confidence: 99%

“…In the pursuit of enhanced stability and a dynamic mechanical response, Yao et al created a hybrid hydrogel that incorporated reversible physical cross-links and photo-polymerizable groups (Yao et al 2014). A reactive cysteine residue was introduced into the sequence of a coiled-coil derived from the five-helix bundle of cartilage oligomeric matrix protein (COMP).…”

Section: Coiled-coil Hydrogelsmentioning

confidence: 99%

“…Photo-induced polymerization of the terminal acrylates afforded a network in which the pentameric coiled-coil acted as a physical cross-link between polymer chains. This process has the advantage of retaining the dynamic physical cross-links associated with the coiled-coil motif, while permitting rapid in situ fabrication of the hydrogel using photo-induced polymerization (Yao et al 2014). Fibroblast cells could be encapsulated within a 3D hydrogel matrix, which was generated in situ via photo-polymerization of an RDG-modified peptide-macromer.…”

Section: Coiled-coil Hydrogelsmentioning

confidence: 99%

See 2 more Smart Citations

Biomaterials Made from Coiled-Coil Peptides

Conticello

Hughes

Modlin

2017

Subcellular Biochemistry

View full text Add to dashboard Cite

The development of biomaterials designed for specific applications is an important objective in personalized medicine. While the breadth and prominence of biomaterials have increased exponentially over the past decades, critical challenges remain to be addressed, particularly in the development of biomaterials that exhibit highly specific functions. These functional properties are often encoded within the molecular structure of the component molecules. Proteins, as a consequence of their structural specificity, represent useful substrates for the construction of functional biomaterials through rational design. This chapter provides an in-depth survey of biomaterials constructed from coiled-coils, one of the best-understood protein structural motifs. We discuss the utility of this structurally diverse and functionally tunable class of proteins for the creation of novel biomaterials. This discussion illustrates the progress that has been made in the development of coiled-coil biomaterials by showcasing studies that bridge the gap between the academic science and potential technological impact.

show abstract

Self‐Healing Biomaterials: From Molecular Concepts to Clinical Applications

Diba

Spaans

Ning

et al. 2018

Adv Materials Inter

View full text Add to dashboard Cite

were selected "off-the-shelf" based on the ingenuity of the surgeons. [1] During the last century, significant medical and technological progress has paved the way for the field of biomaterials research and the emergence of a biomaterials industry. Various synthetic or natural biomaterials have been developed which have enabled treatment of a wide range of medical conditions. Nevertheless, biomaterials are being considered for increasingly complex indications, which has increased the requirements for biomaterials considerably during the past decades. [2] Consequently, challenging-or even contradictorycombinations of biomaterial properties are often required which cannot be met by conventional biomaterials. For instance, biomaterials are desired which combine injectability, mechanical strength, and degradability with toughness to avoid mechanical damage following crack propagation. To meet these strict requirements, biomaterials are needed which can adapt to the implantation site and are able to heal themselves upon mechanical damage. While the majority of synthetic biomaterials does not recover from mechanical damage, natural tissues display a remarkable capacity for self-healing such as the spontaneous self-repair of bone fractures or ruptured skin. This self-healing ability is the ultimate solution of nature for continued survival based Biomaterials are being applied in increasingly complex areas such as tissue engineering, bioprinting, and regenerative medicine. For these applications, challenging-or even contradictory-combinations of biomaterial properties are often required which cannot be met by conventional biomaterials. During the past decade, several new concepts have been developed to render biomaterials self-healing, thereby offering new opportunities to improve the functionality of traditional biomaterials in terms of their mechanical, handling, and biological properties. Consequently, various types of self-healing polymeric, ceramic, or composite biomaterials have been developed. Nevertheless, despite the rapid emergence of the field of self-healing biomaterials, this field of research has not been reviewed during the recent years. Therefore, this article provides a critical overview of recent progress in the field of self-healing biomaterials research by discussing both extrinsic and intrinsic self-healing systems. While the extrinsic self-healing section focuses on self-healing dental materials and orthopedic bone cements that rely on release of healing liquids from embedded microcapsules, the section on intrinsic self-healing materials mainly discusses concepts for self-healing of polymeric biomaterials that are either hydrated (hydrogels) or nonhydrated (e.g., films and coatings). Finally, benefits of the self-healing feature for biomaterials are discussed, and directions for future research and developments are outlined.

show abstract

Polypeptide-engineered physical hydrogels designed from the coiled-coil region of cartilage oligomeric matrix protein for three-dimensional cell culture

Abstract: A class of physical hydrogels photo-cross-linked from multi-branched photopolymeriized monomers based on the self-assembly of coiled-coil polypeptide P is developed.

Cited by 21 publications

References 44 publications

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Biomaterials Made from Coiled-Coil Peptides

Self‐Healing Biomaterials: From Molecular Concepts to Clinical Applications

Contact Info

Product

Resources

About