Polyoxometalate nanostructured gold surfaces for sensitive biosensing of benzo[a]pyrene

Tailor–made Covalent Organic‐Inorganic Polyoxometalate Hybrids: Versatile Platforms for the Elaboration of Functional Molecular Architectures

“…The rIgG / anti‐rIgG ratio was close to 1, which was satisfactory. A similar approach was developed later on for the recognition of small pollutants such as benzo[a]pyrene in the indirect competitive format …”

Rapid fluorescence immunoassay of benzo[a]pyrene in mainstream cigarette smoke based on a dual-functional antibody–DNA conjugate

“…Moreover, for other immunoassay methods that have been reported so far, the redox-labeled electrochemical immunoassay method has an LOD of 2.4 ng mL À1 and a competitive piezoelectric biosensor with 1 mmol L À1 . 14,33,34 In terms of LOD, the RFIA method is more sensitive than these methods.…”

Enhanced Vibrational Spectroscopies as Tools for Small Molecule Biosensing

“…On the spectra, this is evidenced by the decrease of carbonyl vibration band of the carboxylic acids, and by the presence of new carbonyl stretching vibrations: O=C−O−NR group at 1741 cm −1 , and the carbonyl stretching vibrations of the succinimide at 1817 and 1787 cm −1 , respectively. Finally, protein grafting leads to amide bands I and II at ν = 1650 cm −1 and ν = 1550 cm −1 , respectively [ 22 , 23 , 24 ]. On amine-terminated SAMs, the deformation symmetric vibration δ NH of primary ammonium functions are present at 1575 cm −1 together with the stretching vibration δ NH of primary amine functions and the deformation asymmetric vibration δ NH of primary ammonium functions at 1660–1630 cm −1 .…”

“…The unique reported example of direct biosensing of B[a]P was done using PM-IRRAS [ 43 , 44 ] ( Figure 4 ). PMIRRAS made it possible to directly detect PAH compounds thanks to their specific ν CH aromatic signals at ca ν = 3030 cm −1 with a limit of detection below 3 μM [ 22 , 43 . 44 ].…”