Polyomavirus Infections in Adult Psittacine Birds

Ritchie, Branson W.; Niagro, Frank D.; Latimer, Kenneth S.; Vernot, Jacqueline; Pesti, Denise; Campagnoli, Raymond P.; Lukert, P. D.

doi:10.2307/27671072

Cited by 23 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared to these viruses, avian polyomavirus (APV) exhibits unique biological and structural characteristics: whereas mammalian polyomaviruses usually cause innocuous infections in their natural nonimmunocompromised hosts, APV is the causative agent of a fatal multisystemic disease in several species of birds (11,14,29,34), observed all over the world. Recently, an additional structural protein, VP4, was identified within APV particles, a feature which is unique among the polyomaviruses (12).…”

mentioning

confidence: 99%

Nuclear Localization of Avian Polyomavirus Structural Protein VP1 Is a Prerequisite for the Formation of Virus-Like Particles

Johne

Müller

2004

J Virol

View full text Add to dashboard Cite

Virions of polyomaviruses consist of the major structural protein VP1, the minor structural proteins VP2 and VP3, and the viral genome associated with histones. An additional structural protein, VP4, is present in avian polyomavirus (APV) particles. As it had been reported that expression of APV VP1 in insect cells did not result in the formation of virus-like particles (VLP), the prerequisites for particle formation were analyzed. To this end, recombinant influenza viruses were created to (co)express the structural proteins of APV in chicken embryo cells, permissive for APV replication. VP1 expressed individually or coexpressed with VP4 did not result in VLP formation; both proteins (co)localized in the cytoplasm. Transport of VP1, or the VP1-VP4 complex, into the nucleus was facilitated by the coexpression of VP3 and resulted in the formation of VLP. Accordingly, a mutant APV VP1 carrying the N-terminal nuclear localization signal of simian virus 40 VP1 was transported to the nucleus and assembled into VLP. These results support a model of APV capsid assembly in which complexes of the structural proteins VP1, VP3 (or VP2), and VP4, formed within the cytoplasm, are transported to the nucleus using the nuclear localization signal of VP3 (or VP2); there, capsid formation is induced by the nuclear environment.Polyomaviruses are nonenveloped icosahedral particles with a diameter of ϳ45 nm containing a circular double-stranded DNA genome complexed with cellular histones (41). The capsid is composed of the major capsid protein VP1 and the two minor capsid proteins VP2 and VP3, which are expressed late in the viral replication cycle. VP3 is identical to the C-terminal region of VP2; translation is initiated by an internal initiation codon within the VP2-encoding region. Five molecules of VP1 assemble into a pentameric complex which interacts with one molecule of either VP2 or VP3, thus representing a capsomer of the polyomavirus particle. Infectious viral particles, consisting of 72 capsomers and the viral genome, are assembled within the nucleus of the infected cell. Although the structure of the polyomavirus particle has been well characterized by X-ray crystallography (18, 38), the detailed mechanisms of capsid assembly and the process of DNA packaging into the capsid are still subjects of intensive study (7,15,16).Several polyomaviruses infecting different mammalian species have been described, including the human polyomaviruses JC virus (JCPyV) and BK virus (BKPyV), simian virus 40 (SV40), and the murine polyomavirus (MPyV) (41). Compared to these viruses, avian polyomavirus (APV) exhibits unique biological and structural characteristics: whereas mammalian polyomaviruses usually cause innocuous infections in their natural nonimmunocompromised hosts, APV is the causative agent of a fatal multisystemic disease in several species of birds (11,14,29,34), observed all over the world. Recently, an additional structural protein, VP4, was identified within APV particles, a feature which is unique among the polyomaviruses...

show abstract

mentioning

confidence: 99%

Nuclear Localization of Avian Polyomavirus Structural Protein VP1 Is a Prerequisite for the Formation of Virus-Like Particles

Johne

Müller

2004

J Virol

View full text Add to dashboard Cite

show abstract

“…Rabbit polyclonal primary antibody (1:1200 dilution) was used to detect avian polyomavirus infection. A murine monoclonal primary antibody (1:50 dilution) was used to diagnose PBFD virus infection (Ritchie et al, 1991b;Latimer et al, 1992). An indirect immunoperoxidase technique (IIP) was used to demonstrate herpesvirus infection (Pacheco's parrot disease).…”

Section: Standard Disease Diagnosismentioning

confidence: 99%

“…or PBFD virus infections (Ritchie et al, 1991b;Scott et al, 1986;Larimer et al, 1991). Finally, nuclear oedema (K. S. Larimer: personal experience) or the presence of nuclear filaments and granular amorphous material of unknown significance (Pass and Perry, 1984;Jacobson et al, 1986) in hepatocytes may mimic eosinophilic viral inclusions.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of psittacine beak and feather disease (PBFD) viral infection, avian polyomavirus infection, adenovirus infection and herpesvirus infection in psittacine tissues using DNAin situhybridization

et al. 1994

Self Cite

View full text Add to dashboard Cite

SUMMARYThe evaluation of the usefulness of DNA probes in a diagnostic setting to identify nuclear inclusions in selected viral infections (psittacine beak and feather disease viral infection, avian polyomavirus infection, adenovirus infection and Pacheco's parrot disease) is reported. A DNA in situ hybridization method was used to detect viral nucleic acid in sections of paraffin-embedded tissues coming from birds naturally and/or experimentally infected. It is concluded that DNA probes used for polyomavirus and adenovirus (FN-23) are able to identify nucleic acid of each virus in the cells with nuclear inclusions, and when used for psittacine beak and feather disease virus (FN-8), and Pacheco's parrot disease virus (FN-49) are able to detect viral nucleic acid in cells with or without inclusions.

show abstract

“…APV has also been associated with decreased hatchability and embryonic death (17). As a result of several devastating outbreaks in captive birds in North America, Australia, and Europe, APV is now a recognized globally as an avian pathogen (43). A small serological survey in 2002 (25) and a recent confirmed report of APV isolated by PCR from a captive finch (1) suggests that this virus is present in New Zealand; however, the prevalence in caged and wild birds is unknown.…”

mentioning

confidence: 99%

Disease Screening of Three Breeding Populations of Adult Exhibition Budgerigars (Melopsittacus undulatus) in New Zealand Reveals a High Prevalence of a Novel Polyomavirus and Avian Malaria Infection

Baron

Howe²,

Varsani

et al. 2014

Avian Diseases

View full text Add to dashboard Cite

SUMMARY. Disease surveillance is vital to the management of New Zealand's endemic and threatened avian species. Three infectious agents that are potential threats to New Zealand's endemic birds include avian polyomavirus (APV), beak and feather disease virus (BFDV), and avian malaria. All three agents have been reported in New Zealand; however, possible reservoir populations have not been identified. In this communication, we report the first study of APV, BFDV, and avian malaria in introduced adult exhibition budgerigars (Melopsittacus undulatus) in New Zealand. Blood samples were collected from 90 living adult budgerigars from three breeding locations in the North Island of New Zealand. An overall APV prevalence of 22% was determined using a broad-spectrum nested PCR that amplified the major capsid protein VP1 gene of polyomavirus. Phylogenetic analysis of the VP1 gene revealed a unique isolate of APV, which had a sequence divergence of 32% to previously reported budgerigar fledgling disease strains and 33% to the recently reported New Zealand finch isolate. All of the budgerigars sampled were found to be PCR negative for BFDV, and an overall prevalence of 30% was detected by PCR for avian malaria. Sequencing revealed the presence of ubiquitous malarial strains and also the potentially destructive Plasmodium relictum strain. The results of this study suggest that both APV and avian malaria are present in New Zealand adult budgerigars, and our study highlights the need for further studies to determine whether these pathogens in captive bird populations may be a threat or spill over into New Zealand's endemic and threatened avifauna and whether prevention and control methods need to be implemented. RESUMEN. Un estudio de enfermedades de tres poblaciones reproductoras de periquitos australianos (Melopsittacus undulatus)de exhibición en Nueva Zelanda revela una alta prevalencia de una infección por un nuevo poliomavirus y por la malaria aviar.La vigilancia de enfermedades es vital para el manejo de las especies aviares endémicas y amenazadas de Nueva Zelanda. Tres agentes infecciosos que son amenazas potenciales para las aves endémicas de Nueva Zelanda incluyen al poliomavirus aviar (APV), el virus de la enfermedad del pico y plumas (BFDV), y la malaria aviar. Los tres agentes se han reportado en Nueva Zelanda, sin embargo, no han sido identificadas poblaciones de posibles reservorios. En este artículo se presenta el primer estudio de poliomavirus aviar, del virus del pico y las plumas y de la malaria aviar en periquitos australianos (Melopsittacus undulatus) adultos de exhibición introducidos a Nueva Zelanda. Se recolectaron muestras de sangre de 90 periquitos adultos vivos de tres lugares de cría en la Isla Norte de Nueva Zelanda. Se determinó una prevalencia general de poliomavirus aviar de 22% utilizando un método de PCR anidado que amplifica el gene de la proteína principal de la cápside VP1 del poliomavirus. El análisis filogenético del gene VP1 reveló un aislamiento único de poliomavirus, que tenía una dive...

show abstract

Polyomavirus Infections in Adult Psittacine Birds

Cited by 23 publications

References 0 publications

Nuclear Localization of Avian Polyomavirus Structural Protein VP1 Is a Prerequisite for the Formation of Virus-Like Particles

Nuclear Localization of Avian Polyomavirus Structural Protein VP1 Is a Prerequisite for the Formation of Virus-Like Particles

Diagnosis of psittacine beak and feather disease (PBFD) viral infection, avian polyomavirus infection, adenovirus infection and herpesvirus infection in psittacine tissues using DNAin situhybridization

Disease Screening of Three Breeding Populations of Adult Exhibition Budgerigars (Melopsittacus undulatus) in New Zealand Reveals a High Prevalence of a Novel Polyomavirus and Avian Malaria Infection

Contact Info

Product

Resources

About