Polyol production from waste materials by genetically modified Yarrowia lipolytica

Rakicka, Magdalena; Biegalska, Anna; Rymowicz, Waldemar; Dobrowolski, Adam; Mirończuk, Aleksandra M.

doi:10.1016/j.biortech.2017.06.137

Cited by 66 publications

(45 citation statements)

References 41 publications

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“…With the aid of Y. lipolytica strains, biodiesel waste can be converted into microbial protein [26], lipids [27][28][29][30], polyols [31,32], ketoacids [33,34], as well as citric acid (CA) and its salts [35][36][37][38]. In addition to CA, the wild strains Y. lipolytica cultivated on biodiesel waste accumulates ICA in sufficient amounts [39,40].…”

Section: Introductionmentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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Within this work, the microbial synthesis of (2R,3S)-isocitric acid (ICA), a metabolite of the nonconventional yeast Yarrowia lipolytica, from biodiesel waste, has been studied. The selected strain Y. lipolytica VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions. The maximal production of ICA with the minimal formation of the byproduct was provided by the use of a concentration of (NH4)2SO4 (6 g/L); the addition of biodiesel waste to cultivation medium in 20–60 g/L portions; maintaining the pH of the cultivation medium at 6, and degree of aeration between 25% and 60% of saturation. Itaconic acid at a concentration of 15 mM favorably influenced the production of ICA by the selected strain. The optimization of cultivation conditions allowed us to increase the concentration of ICA in the culture liquid from 58.32 to 90.2 g/L, the product yield (Y) by 40%, and the ICA/CA ratio from 1.1:1 to 3:1. Research on laboratory animals indicated that ICA counteracted the negative effect of ammonium molybdate (10−5 М) and lead diacetate (10−7 М) on the learning and spatial memory of rats, including those exposed to emotional stress.

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Section: Introductionmentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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“…This allowed an EOL titer of 14.7 g/L with a yield of 0.49 g/g in a 5 L bioreactor within 144 h [108]. By overexpressing SUC2 genes from Saccharomyces cerevisiae encoding invertase and native GUT1 gene (YALI0F00384g), encoding glycerol kinase (GK), EOL was produced from raw industrial molasses (60 g/L) and crude glycerol (100 g/L) and reached a titer of 100 g/L with a productivity and yield of 1.1 g/(L•h) and a yield of 0.67 g/g [109].…”

Section: Erythritolmentioning

confidence: 99%

“…With glycerol fed-batch culture, a strain A UV'1 and A-15 produced mannitol with a titer of 38.1 and 41.4 g/L, respectively, within 150 h. This corresponds to a productivity and yield of 0.29 g/(L•h) and 0.15 g/g; and 0.28 g/(L•h) and 0.17 g/g, respectively [117]. With a mutant strain AIB pAD-UTGut, a derivative of strain AIB that overexpressed GUT1 gene, a mannitol titer of 11 g/L was obtained in a batch bioreactor on a mixture of crude glycerol and molasses [109]. With the Y. lipolytica strain ACA-DC 5029 grown on crude glycerol (70 g/L) blended with OMW, a mannitol titer of 13 g/L in 140 h with a yield of 0.21 g/g glycerol was obtained [118].…”

Section: Other Polyolsmentioning

confidence: 99%

“…With strain A UV'1 and A-15, ARA titer ranging from 0.3 to 5.6 g/L were obtained from crude glycerol in a shake flask culture [117]. For an engineered strain AIB pAD-UTGut, a derivative of strain AIB that overexpressed GUT1 gene, an arabitol titer of 7.5 g/L was obtained in a batch bioreactor using a mixture of crude glycerol and molasses [109]. When OMW was blended with 70 g/L glycerol, the Y. lipolytica strain ACA-DC 5029 produced ARA with a titer of 4.5 g/L in 264 h with a yield of 0.05 g/g glycerol [118].…”

Section: Other Polyolsmentioning

confidence: 99%

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Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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“…Y. lipolytica can grow on a broad range of substrates and convert various organic waste to high-value chemicals (Dobrowolski, Mituła, Rymowicz, & Mirończuk, 2016;Rakicka, Biegalska, Rymowicz, Dobrowolski, & Mirończuk, 2017). For example, it has been reported that Y. lipolytica possessed strong acetate utilization pathway that is equivalent or even superior to the hexose utilization pathway, which led to an improvement of triacylglyceride (TAG) production from 100 g/L to 115 g/L in bench-top bioreactors, when the cultivation was switched from glucose media to acetate media J.…”

Section: Glucose and Acetate As Media For Squalene Productionmentioning

confidence: 99%

Optimizing mevalonate pathway for squalene production in Yarrowia lipolytica

Liu

Wang

Deng

et al. 2020

Preprint

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Li Deng) and pengxu@umbc.edu (PX). AbstractSqualene is the gateway molecule for triterpene-based natural products and steroidsbased pharmaceuticals. As a super lubricant, it has been used widely in health care industry due to its skin compatibility and thermostability. Squalene is traditionally sourced from sharkhunting or oil plant extraction, which is cost-prohibitive and not sustainable. Reconstitution of squalene biosynthetic pathway in microbial hosts is considered as a promising alternative for cost-efficient and scalable synthesis of squalene. In this work, we reported the engineering of the oleaginous yeast, Y. lipolytica, as a potential host for squalene production. We systematically identified the bottleneck of the pathway and discovered that the native HMG-CoA reductase led to the highest squalene improvement. With the recycling of NADPH from the mannitol cycle, the engineered strain produced about 180.3 mg/l and 188.2 mg/L squalene from glucose or acetate minimal media, respectively. By optimizing the C/N ratio, controlling the media pH and mitigating the acetyl-CoA flux competition from lipogenesis, the engineered strain produced about 502.7 mg/L squalene in shake flaks, a 28-fold increase compared to the parental strain (17.2 mg/L). We also profiled the metabolic byproducts citric acid and mannitol level and observed that they are reincorporated into cell metabolism at the late stage of fermentation. This work may serve as a baseline to harness Y. lipolytica as an oleaginous cell factory for production of squalene or terpene-based chemicals.

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Polyol production from waste materials by genetically modified Yarrowia lipolytica

Cited by 66 publications

References 41 publications

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

Optimizing mevalonate pathway for squalene production in Yarrowia lipolytica

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