Polynuclear aromatic anthracene biodegradation by psychrophilic Sphingomonas sp., cultivated with tween-80

Farraj, Dunia A. Al; Alkufeidy, Roua M.; Alkubaisi, Noorah A.; Alshammari, Maryam K.

doi:10.1016/j.chemosphere.2020.128115

Cited by 24 publications

(8 citation statements)

References 25 publications

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“…Additionally, Bjerkandera adusta degraded 38% of anthracene during three days of incubation [56] and 70.5% of anthracene (50 µg/mL) was degraded by Sphingomonas sp. KSU05 at 96 h of cultivation [14]. Surprisingly, our isolates showed a high potential to biodegrade anthracene under aerobic conditions within six days as 94.58%, 94.53%, and 99.61% of the starting anthracene concentration were degraded by Brevibacillus sp., Pseudomonas sp., and Methylocystis sp., respectively.…”

Section: Discussionmentioning

confidence: 74%

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Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Magdy

Gaber

Sebak

et al. 2022

Beni-Suef Univ J Basic Appl Sci

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Background Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation of these xenobiotics is a cost-effective technological solution. The present study aimed to recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities of anthracene biodegradation. The selected isolates were molecularly characterized by 16S rRNA gene sequencing, the degree of anthracene biodegradation was monitored using optical density (OD) and high-performance liquid chromatography (HPLC), PCR amplification of some selected genes encoding biodegradation of PAHs was monitored, and gas chromatography–mass spectrometry (GC–MS) analysis was applied for detecting the resulted metabolites. Result Three bacterial isolates were studied, the 16s rRNA sequences of the isolates showed homology of the first isolate to Brevibacillus sp. (94.58 %), the second isolates showed homology to Pseudomonas sp. (94.53%) and the third isolate showed homology to Methylocystis sp. (99.61 %), all isolates showed the ability to degrade anthracene. PCR amplification of some selected genes encoding biodegradation of PAHs revealed the presence of many biodegrading genes in the selected strains. Gas chromatography-mass spectrometry (GC–MS) analysis of the metabolites resulted from anthracene biodegradation in the present study suggested that more than one biodegradation pathway was followed by the selected isolates. Conclusions The selected strains could represent a potential bioremediation tool in solving the PAHs problem in the Egyptian environment with a clean and cost-effective technique. Graphical Abstract

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Section: Discussionmentioning

confidence: 74%

“…Anthracene is used as a signature compound in PAHs biodegradation studies [12][13][14]. It is composed of three fused benzene rings [15].…”

Section: Introductionmentioning

confidence: 99%

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Magdy

Gaber

Sebak

et al. 2022

Beni-Suef Univ J Basic Appl Sci

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“…Lastly, it was recently reported that the addition of Tween ® 80 to the culture medium enhanced anthracene biodegradation by Sphingomonas sp. [ 31 , 32 , 33 ]. However, the physiological and/or biophysical mechanisms underlying most of Tween ® 80’s above-mentioned effects have not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

A Unique Enhancement of Propionibacterium freudenreichii’s Ability to Remove Pb(II) from Aqueous Solution by Tween 80 Treatment

George

Titécat

Barois

et al. 2022

IJMS

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Microbial agents have promise for the bioremediation of Pb(II)-polluted environments and wastewater, the biodecontamination of foods, and the alleviation of toxicity in living organisms. The dairy bacterium Propionibacterium freudenreichii is poorly able to remove Pb(II) from aqueous solution at 25 ppm, ranging from 0 to 10% of initial concentration. Here, we report on an original strong enhancement of this activity (ranging from 75% to 93%, p < 0.01) following the addition of a polysorbate detergent (Tween® 80) during or either shortly after the growth of a P. freudenreichii culture. We evaluated the optimal Tween® 80 concentration for pretreatment conditions, documented the role of other detergents, and explored the possible mechanisms involved. Our results reveal a novel, environmentally friendly, low-cost pretreatment procedure for enhancing the selective removal of lead from water by probiotic-documented bacteria.

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“…Polycyclic aromatic hydrocarbons are a group of persistent organic pollutants with intensive toxicity and wide distribution, posing a serious threat to environmental and human health ( Xue and Warshawsky, 2005 ; Couling et al, 2010 ; Zhou et al, 2018 ). Previous investigations have reported that co-metabolism triggered by glucose could significantly accelerate the degradation of PAHs under both aerobic and anaerobic digestion environments ( Ambrosoli et al, 2005 ; Ma et al, 2011 ; Qin et al, 2018 ; Tripathi et al, 2020 ; Al Farraj et al, 2021 ). For example, the addition of glucose was reported to significantly enhance the degradation of PAHs (benzopyrene, anthracene, and pyrene) when treated with some pure bacterial species, such as Cellulosimicrobium , Sphingomonas , and Bacillus ( Khanna et al, 2012 ; Qin et al, 2018 ; Al Farraj et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Previous investigations have reported that co-metabolism triggered by glucose could significantly accelerate the degradation of PAHs under both aerobic and anaerobic digestion environments ( Ambrosoli et al, 2005 ; Ma et al, 2011 ; Qin et al, 2018 ; Tripathi et al, 2020 ; Al Farraj et al, 2021 ). For example, the addition of glucose was reported to significantly enhance the degradation of PAHs (benzopyrene, anthracene, and pyrene) when treated with some pure bacterial species, such as Cellulosimicrobium , Sphingomonas , and Bacillus ( Khanna et al, 2012 ; Qin et al, 2018 ; Al Farraj et al, 2021 ). Additionally, PAH degradation under methanogenesis is thermodynamically feasible and the degradation of PAHs can be coupled with methanogenesis, suggesting that remediation of PAHs pollution via the anaerobic digestion system should be a potentially ideal method marked by the properties of energy conservation and resource reuse ( Chang et al, 2005 ; Dolfing et al, 2009 ; Berdugo-Clavijo et al, 2012 ; Wan et al, 2012 ; Nzila, 2018 ; Ye et al, 2019 ; Wang et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Co-metabolic Effect of Glucose on Methane Production and Phenanthrene Removal in an Enriched Phenanthrene-Degrading Consortium Under Methanogenesis

Zhou

Wang

et al. 2021

Front. Microbiol.

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Anaerobic digestion is used to treat diverse waste classes, and polycyclic aromatic hydrocarbons (PAHs) are a class of refractory compounds that common in wastes treated using anaerobic digestion. In this study, a microbial consortium with the ability to degrade phenanthrene under methanogenesis was enriched from paddy soil to investigate the cometabolic effect of glucose on methane (CH4) production and phenanthrene (a representative PAH) degradation under methanogenic conditions. The addition of glucose enhanced the CH4 production rate (from 0.37 to 2.25mg⋅L−1⋅d−1) but had no influence on the degradation rate of phenanthrene. Moreover, glucose addition significantly decreased the microbial α-diversity (from 2.59 to 1.30) of the enriched consortium but showed no significant effect on the microbial community (R2=0.39, p=0.10), archaeal community (R2=0.48, p=0.10), or functional profile (R2=0.48, p=0.10). The relative abundance of genes involved in the degradation of aromatic compounds showed a decreasing tendency with the addition of glucose, whereas that of genes related to CH4 synthesis was not affected. Additionally, the abundance of genes related to the acetate pathway was the highest among the four types of CH4 synthesis pathways detected in the enriched consortium, which averagely accounted for 48.24% of the total CH4 synthesis pathway, indicating that the acetate pathway is dominant in this phenanthrene-degrading system during methanogenesis. Our results reveal that achieving an ideal effect is diffcult via co-metabolism in a single-stage digestion system of PAH under methanogenesis; thus, other anaerobic systems with higher PAH removal efficiency should be combined with methanogenic digestion, assembling a multistage pattern to enhance the PAH removal rate and CH4 production in anaerobic digestion.

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Polynuclear aromatic anthracene biodegradation by psychrophilic Sphingomonas sp., cultivated with tween-80

Cited by 24 publications

References 25 publications

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

A Unique Enhancement of Propionibacterium freudenreichii’s Ability to Remove Pb(II) from Aqueous Solution by Tween 80 Treatment

Co-metabolic Effect of Glucose on Methane Production and Phenanthrene Removal in an Enriched Phenanthrene-Degrading Consortium Under Methanogenesis

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