Polymyxin B effects on motility parameters of cryopreserved bull semen

Rashedi, Mojtaba; Fazeli, Mohammad Hashem; Gholami, Hamid; Bahreini, Mohammad

doi:10.12980/apjr.6.20170107

Cited by 1 publication

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References 33 publications

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“…Treatment of boar spermatozoa with high concentrations of PMB (75 or 100 μM) resulted in the decrease in viability (Figure b). A recent study showed that addition of PMB to bull semen extender at 100 μg/ml (equal to ~75 μM) resulted in a beneficial effect on sperm motility after freezing‐thawing while a higher concentration (1,000 μg/ml, equal to ~750 μM) of PMB reduced viability (Rashedi, Fazeli, Gholami, & Bahreini, ). This indicates that the antibiotic is spermicidal depending on the concentrations used.…”

Section: Discussionmentioning

confidence: 99%

Polymyxin B enhances acrosomal exocytosis triggered by calcium and the calcium ionophore A23187 in ejaculated boar spermatozoa

Akter

Rajabi-Toustani

Shimizu³

et al. 2019

Animal Science Journal

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Polymyxin B (PMB) is beneficial for boar semen storage since it neutralizes the endotoxin of bacteria. However, the direct effect of PMB on boar spermatozoa has been unknown. This study aimed to examine the effect of PMB on acrosomal exocytosis, an essential process for successful fertilization in boar spermatozoa. Ejaculated spermatozoa stored with BTS extender at 17°C were washed and incubated with 0–100 μM PMB for 20 min and then examined for % total motililty, vigor grade and viability. None of the parameters was significantly different between 0 and 50 μM PMB with a gradual decline at higher concentrations. Thus the effect on acrosomal exocytosis was investigated at 0–50 μM of PMB. Spermatozoa were preincubated with PMB for 10 min, incubated for stimulation of acrosomal exocytosis with Ca2+ and the calcium ionophore A23187 and then fixed with glutaraldehyde at 5, 10 and 15 min. Preincubation with PMB at 0.01–50 μM and 0.05–50 μM resulted in significant enhancement of acrosomal exocytosis at 10 min and 15 min of incubation, respectively. Preincubation with PMB followed by incubation without A23187 did not affect acrosomal exocytosis. These results suggest that PMB exerts effects on the acrosomal exocytosis triggered by Ca2+ and A23187 in boar spermatozoa.

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Section: Discussionmentioning

confidence: 99%