Polymorphonuclear Neutrophil Infiltration Intensity as Consequence of<i>Entamoeba histolytica</i>Density in Amebic Colitis

Dickson-Gonzalez, Sonia M.; Uribe, M.; Rodríguez-Morales, Alfonso J.

doi:10.1089/sur.2008.011

Cited by 20 publications

(18 citation statements)

References 31 publications

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“…The causative protozoan parasite, E. histolytica (Figure 1), is a potent pathogen, transmitted via ingestion of the cystic mature form (Figure 2), the infective stage of this protozoan parasite (Dickson-Gonzalez, et al 2009). Fig.…”

Section: Pathologymentioning

confidence: 99%

“…1. Entamoeba histolytica throphozoites, showing nucleus and karyosoma (arrow) (HF, 1000X) (Dickson-Gonzalez et al, 2009) Viable in the environment for weeks to even months, cysts can be found in fecally contaminated soil, fertilizer, or water or on the contaminated hands of food handlers and contaminated elements that can be used during food preparation or ingestion (DicksonGonzalez, et al 2009).…”

Section: Pathologymentioning

confidence: 99%

“…7. Interglandular corion with abundant inflammatory infiltrate rich in PMN and eosinophils (HE, 400X, in the zooming 1000X) (Dickson-Gonzalez et al, 2009) Molecular biology techniques can directly identify RNA and DNA of the parasite, being specific for the species. Right now using multiple targeting genes, including a small-subunit rRNA gene (18S rDNA), serine-rich protein gene, chitinase gene, 30-kDa antigen gene, hemolysin gene, and extrachromosomal circular DNA, among others, E. histolytica can be differentiated from other species.…”

Section: Differential Diagnoses Of Amebiasismentioning

confidence: 99%

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Amebic Colitis

Carolina¹,

Escudero-Sepulveda²,

Alfonso³

2012

Colitis

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Section: Pathologymentioning

confidence: 99%

Section: Pathologymentioning

confidence: 99%

Section: Differential Diagnoses Of Amebiasismentioning

confidence: 99%

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Amebic Colitis

Carolina¹,

Escudero-Sepulveda²,

Alfonso³

2012

Colitis

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“…Consequently, several reports suggest that neutrophils play a protective role in amoebiasis . However, other reports indicate that trophozoites making direct contact with neutrophils provoke lysis of these leukocytes, resulting in the release of their lytic enzymes, which in turn provoke tissue damage . Therefore, the interaction of neutrophils with this parasite is complex and not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation

Fonseca

Uribe‐Querol

Díaz-Godínez

et al. 2019

Journal of Leukocyte Biology

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Amoebiasis is an infection of global importance, caused by the eukaryotic parasite Entamoeba histolytica. Pathogenic E. histolytica is associated worldwide with over a million cases of amoebic dysentery, colitis, and amoebic liver abscess. In contrast, the nonpathogenic Entamoeba dispar does not cause these diseases, although it is commonly found in the same areas as pathogenic amoeba. Entamoeba histolytica infection is usually associated with infiltrating neutrophils. These neutrophils appear to play a defensive role against this parasite, by mechanisms not completely understood. Recently, our group reported that neutrophil extracellular traps (NET) are produced in response to E. histolytica trophozoites. But, there is no information on whether nonpathogenic E. dispar can also induce NET formation. In this report, we explored the possibility that E. dispar leads to NET formation. Neutrophils were stimulated by E. histolytica trophozoites or by E. dispar trophozoites, and NET formation was assessed by video microscopy. NET induced by E. histolytica were important for trapping and killing amoebas. In contrast, E. dispar did not induce NET formation in any condition. Also E. dispar did not induce neutrophil degranulation or reactive oxygen species production. In addition, E. histolytica‐induced NET formation required alive amoebas and it was inhibited by galactose, N‐acetylgalactosamine, and lactose. These data show that only alive pathogenic E. histolytica activates neutrophils to produce NET, and suggest that recognition of the parasite involves a carbohydrate with an axial HO‐ group at carbon 4 of a hexose.

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“…Neutrophils are infiltrated in the inflammatory site at early stages of amebiasis [4]. Even though some studies in mice reported that neutrophils are not important to eliminate E. histolytica infection from the intestine [5], recent studies show that neutrophils are accumulated in colonic mucosa of patients with amebic colitis [6,7] and support that neutrophils play a vital role in resistance to amebic liver abscess and amebic colitis [4,8]. While host immune cells have various mechanisms for eliminating pathogens, E. histolytica has also developed defense strategies for its survival.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica

Sim

Lee

et al. 2010

Korean J Parasitol

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Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica. In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2. Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica.

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Polymorphonuclear Neutrophil Infiltration Intensity as Consequence ofEntamoeba histolyticaDensity in Amebic Colitis

Abstract: Neutrophils and lymphocytes, particularly the former, are associated significantly with the density of parasites. Our findings support the theory that PMN interaction with E. histolytica contributes to the pathogenesis of amebic intestinal lesions.

Cited by 20 publications

References 31 publications

Amebic Colitis

Amebic Colitis

Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation

Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica

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