Polymorphonuclear leukocyte injury by methylglyoxal and hydrogen peroxide: a possible pathological role for enhanced oxidative stress in chronic kidney disease

Nakayama, Masaaki; Nakayama, Keiichi I.; Zhu, Wan Jun; Shirota, Yuko; Terawaki, Hiroyuki; Sato, Toshinobu; Kohno, Masahiro; Ito, Sadayoshi

doi:10.1093/ndt/gfn218

Cited by 18 publications

(16 citation statements)

References 29 publications

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“…MGO is highly reactive and biologically toxic due to the formation of advanced glycation end products, protein and DNA modifications [21,22,23]. Recently, we demonstrated that MGO plays a role in the enhancement of injury to PMNs in combination with H 2 O 2 [24], which may be involved with the pathological mechanism of microinflammation in dialysis patients.…”

Section: Discussionmentioning

confidence: 99%

Biological Effects of Electrolyzed Water in Hemodialysis

Nakayama

Kabayama²,

Nakano³

et al. 2009

Nephron Clin Pract

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Background/Aims: The application of electrolyzed water (EW) at the cathode side to manufacture reverse osmosis (RO) water and hemodialysis (HD) solution can actually lead to less oxidative capacity in chemical terms. The present study examined the biological actions of this water on human polymorphonuclear leukocytes (PMNs), and the clinical feasibility of applying this technology to HD treatment. Methods: RO water using EW (e-RO) exhibited less chemiluminescence in luminol-hydrogen peroxide and higher dissolved hydrogen levels (–99.0 ppb) compared with control RO water. The effects of e-RO on PMN viability were tested. HD using e-RO was performed for 12 consecutive sessions in 8 patients for the feasibility test. Results: Basal cellular viability and function to generate superoxide radicals of PMNs were better preserved by e-RO application. In the clinical trial, reductions of blood pressure were noted, but no adverse events were observed. There were no changes in the blood dialysis parameters, although methylguanidine levels were significantly decreased at the end of study. Conclusion: The present study demonstrated the capacity of e-RO to preserve the viability of PMNs, and the clinical feasibility of applying this water for HD treatment. The clinical application of this technology may improve the bio-compatibility of HD treatment.

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Section: Discussionmentioning

confidence: 99%

Biological Effects of Electrolyzed Water in Hemodialysis

Nakayama

Kabayama²,

Nakano³

et al. 2009

Nephron Clin Pract

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“…Recently, methylglyoxal, an alpha-oxoaldehyde whose levels are increased in CRF, has been shown to induce apoptosis in different cell lines and to generate radicals by reacting with hydrogen peroxide, and therefore it has been postulated to act in a pivotal role in the onset of oxidative injury in CRF [36] . In light of these considerations, the evaluation of leukocyte count and function could become an easy and useful tool to verify the clinical and dialytic outcome of these hemodialysis subjects.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Metabolites, Leukocyte Activation Markers and Oxidative Status in Dialyzed Subjects

Caimi

Carollo²,

Montana³

et al. 2009

Blood Purif

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Aims: Our purpose was to evaluate, in a group of 42 end-stage renal disease patients who regularly undergo hemodialysis, some indexes of leukocyte activation, nitric oxide metabolites (NOx) and other parameters that reflect the oxidative stress before and after a standard hemodialysis session. Methods: Elastase and myeloperoxidase (MPO) were determined by means of ELISA. The NO production was evaluated by a micromethod which measures the concentration of NOx. The oxidation of polyunsaturated fatty acids was evaluated in plasma by detection of thiobarbituric acid-reactive substances (TBARS). Total antioxidant status (TAS) was obtained using spectrophotometry. Results: At baseline, we observed an increase of elastase, NOx, TBARS and TAS, without any variation of MPO. After the dialysis session, we found an increase in elastase and MPO, a decrease in NOx and TAS and no variation in TBARS. No modification occurred after subdividing the patients in two subgroups. Conclusions: Our data confirm the involvement of leukocytes and oxidative stress in hemodialysis patients.

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“…Table 1 lists the most commonly utilized biomarkers in human and experimental models. The application of these biomarkers has now unequivocally demonstrated that acute kidney injury, chronic kidney disease, and end-stage renal disease are states of increased oxidative stress [60][61][62][63][64][65]. A more complete approach with potential promise is the utilization of multiple biomarkers from both oxidant-generating and antioxidant pathways to assess oxidative stress [60,66].…”

Section: Assessment Of Oxidative Stress-biomarker Pitfallsmentioning

confidence: 99%

Tipping the redox balance of oxidative stress in fibrogenic pathways in chronic kidney disease

Okamura

Himmelfarb

2009

Pediatr Nephrol

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Patients with moderate to advanced chronic kidney disease or end-stage renal disease have a greatly increased cardiovascular risk that cannot be explained entirely by traditional cardiovascular risk factors. An increase in oxidative stress and inflammation have been proposed as nontraditional cardiovascular risk factors in this patient population. Oxidative stress reflects the redox balance between oxidant generation and antioxidant mechanisms. The generation of reactive oxygen species is not simply a random process that oxidizes nearby macromolecules, but, in many instances, the oxidants target particular amino acid residues or lipid moieties. Oxidant mechanisms are now recognized to be intimately involved in cell signaling and to be vital components of the immune response. This is equally true for antioxidant mechanisms as well. In the progression of chronic kidney disease, the redox balance is not in equilibrium and is tipped toward oxidation, resulting in the dysregulation of cellular process and subsequent tissue injury. In this review we discuss the major oxidant and antioxidant pathways and the biomarkers to assess redox status. We also review the data linking the pathogenesis of oxidative stress, inflammation, and the progressive loss of kidney function in chronic kidney disease.

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Polymorphonuclear leukocyte injury by methylglyoxal and hydrogen peroxide: a possible pathological role for enhanced oxidative stress in chronic kidney disease

Abstract: These results indicate the combinatory effect of MGO and H(2)O(2) on PMN oxidative injury, and this pathology may be linked to enhanced oxidative stress in CKD.

Cited by 18 publications

References 29 publications

Biological Effects of Electrolyzed Water in Hemodialysis

Biological Effects of Electrolyzed Water in Hemodialysis

Nitric Oxide Metabolites, Leukocyte Activation Markers and Oxidative Status in Dialyzed Subjects

Tipping the redox balance of oxidative stress in fibrogenic pathways in chronic kidney disease

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