Polymorphonuclear leukocyte collagenase: Localization in granules subjected to zonal centrifugation

Abstract

Abstract. Polymorphonuclear leukocytes were obtained from rabbit peritoneal exudates. Granule preparations from these cells were subjected to zonal fractionation through a discontinuous sucrose gradient. Azurophilic and specific granules were enzymatically characterized by peroxidase and alkaline phosphatase, respectively. Collagenase was consistently associated with the alkaline phosphatase‐containing specific granules.

Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)

“…Besides myeloperoxidase, other lysosomal enzymes such as acid and alkaline phosphatases were reported to be involved in the phagocytic process as microorganism disruptors [30,31].…”

Collagenolysis by Human Gingival Fibroblast Cell Lines

“…Furthermore, collagenolytic activity of human gingival crevicular fluid is also demonstrable.8 However, in analyzing pieces of gingival tissue, it is oftcrs difficult to sift out the collagenolytic effects of bacteria.9"10 leukocytes, [11][12][13][14] other types of cells,15 and the collagenase inhibitor effects of serum. 16 Fibroblasts isolated fromn primary cultures of human skin gave an immunofluorescent response with monospecific antiserums to human skin collagenase, and the culture medium contained material immunologically identical to skin collagenase.17 Primary cultures of human corneal fibroblasts grown in tissue culture also released collagenase into the nutrient, but only after an inducement by dexamethasone, although they normally produced a procollagenase activated by trypsin or lysosomal lysates.…”