Polymorphonuclear chemiluminescence: Some clinical applications

Sole, P. De

doi:10.1002/bio.1170040136

Cited by 17 publications

(11 citation statements)

References 41 publications

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“…The light emission is due to the formation of reactive oxygen species in stimulated cells (1-3). There have been many attempts to use LCL of cells for clinical investigation of a number of diseases (4). As well as pathological states with deficiencies in PMN enzymes, the role of neutrophils in such common diseases as myocardial injury and rheumatoid arthritis has been studied.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Arnhold

Mueller

Arnold

et al. 1993

J. Biolumin. Chemilumin.

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Two different mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite were found. Most substances investigated in these experiments acted by scavenging NaOCl. This mechanism was independent of the concentration of hydrogen peroxide and the incubation time between luminol and inhibitors. The most potent inhibitors were substances containing SH groups. Compounds with amino groups as a target for HOCl/OCl- to yield chloramines were much less effective inhibitors. Another mechanism of inhibition was found for catalase. It depended on the presence of hydrogen peroxide in the incubation medium and the incubation time between luminol and catalase. The enzyme inhibited the luminescence by removing H2O2 at molar concentrations much smaller than those found for all other inhibitors. Our results confirm the present models of the mechanism of generation of luminescence in luminal oxidation.

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Section: Introductionmentioning

confidence: 99%

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Arnhold

Mueller

Arnold

et al. 1993

J. Biolumin. Chemilumin.

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“…thoroughly studied and used in both laboratory and clinical practice [for review, see (3)(4)(5)]. However, various authors use different CL methods to detect ROS produced by phagocytes of animals, which makes comparing their results difficult (6)(7)(8)(9)(10)(11)(12)(13)(14).…”

mentioning

confidence: 99%

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Pavelková

Kubala

2004

Luminescence

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Luminol-, isoluminol- or lucigenin-enhanced chemiluminescence (CL) was used to measure the production of reactive oxygen species by rat blood leukocytes. Opsonized zymosan (OZ), phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 (Ca-I) or N-formyl-Met-Leu-Phe (fMLP) were used as activators. The CL signal of isolated blood leukocytes decreased in rank order of luminol > isoluminol > lucigenin. The kinetic profiles of luminol- and isoluminol-enhanced CL were similar upon stimulation by each activator tested. The remarkably higher luminol and isoluminol CL responses were obtained after OZ stimulation when compared with other activators. However, when lucigenin was used, the PMA- and OZ-stimulated CL were comparable. The presence of plasma increased OZ-activated CL because of the enhanced phagocytosis of OZ. This was demonstrated by determining the phagocytosis of the fluorescent OZ using a flow cytometer. In contrast, the presence of plasma decreased PMA-activated CL, due to the antioxidant properties of plasma as determined by the CL method. As far as whole blood is concerned, only OZ activated luminol-enhanced CL was reliable. Blood volumes over 5 microL decreased CL activity due to the scavenging ability of erythrocytes. The results suggest that 0.5 microL whole blood is sufficient for routine luminol-enhanced CL analysis of whole blood oxidative burst in rats.

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“…The magnitude of the neutrophil ROM responses has been reported to correlate with the severity of asthma symptoms [19]. The chemiluminescence signal obtained from whole blood is predominantly a function of neutrophil number and function [33], whilst the contribution of monocytes, lymphocytes and thrombocytes has been considered negligible [22,[34][35][36][37]. We observed no close correlation between total granulocyte count and the PMA-induced whole blood chemiluminescence values, which suggests that the chemiluminescence responses were not merely a function of neutrophil number, but also of neutrophil metabolic activity.…”

Section: Discussionmentioning

confidence: 99%

Increased PMA-induced chemiluminescence from whole blood of patients with bronchial hyperreactivity

Nordman¹,

Nyberg²,

Linko³

1994

Eur Respir J

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Blood phagocytes from patients with asthma have an increased capacity to produce reactive oxygen metabolites. We studied whether whole blood luminol-dependent chemiluminescence could detect this phenomenon in patients with a normal spirometry but bronchial hyperreactivity as determined with a methacholine bronchial challenge test. Whole blood chemiluminescence, serum eosinophilic cationic protein (ECP), and serum myeloperoxidase (MPO) were determined from 50 patients referred for a methacholine challenge due to prolonged cough and/or dyspnoea. The chemiluminescence results were compared to those from 15 healthy persons. The hyperreactive patients (n = 18) had significantly higher phorbol 12-myristate 13-acetate (PMA)-induced whole blood chemiluminescence values (mean 18.8 mV.min-1; 95% confidence limits (C.L.) 16.3-21.3 mV.min-1) than the normoreactive patients (mean 14.2 mV.min-1; 95% C.L. 13.0-15.5 mV.min-1;) and the healthy controls (mean 12.8 mV.min-1; 95% C.L. 11.7-13.9 mV.min-1). There was no significant difference in PMA-induced chemiluminescence between the normoreactive patients and the controls. The hyperreactive patients had higher serum ECP values than the normoreactive patients, but there was no correlation between whole blood chemiluminescence and serum ECP levels or total eosinophil count. There was no significant difference in monocyte reactive oxygen metabolite production or serum MPO values between the normoreactive and the hyperreactive patients. We suggest that the increased PMA-induced whole blood chemiluminescence in bronchial hyperreactivity is due mainly to an activation of neutrophils, and that the assay might be useful as a systemic inflammatory marker in patients with pulmonary inflammatory processes resulting in bronchial hyperreactivity.

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Polymorphonuclear chemiluminescence: Some clinical applications

Cited by 17 publications

References 41 publications

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Luminol‐, isoluminol‐ and lucigenin‐enhanced chemiluminescence of rat blood phagocytes stimulated with different activators

Increased PMA-induced chemiluminescence from whole blood of patients with bronchial hyperreactivity

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