Polymorphisms of the uridine-diphosphoglucuronosyltransferase 1A1 gene and coronary artery disease

Abstract

Abstract:Bilirubin, an antioxidant in the blood, plays a role in protection from atherosclerosis. The level of bilirubin is highly correlated to the incidence of coronary artery disease (CAD). Unconjugated bilirubin is conjugated with glucuronic acid through the reaction of uridine 5'-diphosphate-glucuronosyl transferase 1A1 (UGT1A1). The interactions of CAD and the variations in the coding regions of the UGT1A1 gene have never been evaluated. The purpose of this study was to analyze the influence of the UGT1A1 variant on the incidence of CAD. There were 135 participants in this study: 61 in the experimental group, who had CAD, and 74 in the control group, who did not have CAD. The blood samples from all 135 participants were collected and assayed to clarify the relationship between bilirubin and CAD. The assay of the polymerase chain reaction and the sequence of the UGT1A1 gene were examined to find the gene's polymorphisms. The bilirubin levels for the participants in the control group were significantly higher than for the patients in the CAD group. Although the concentration of bilirubin in the UGT1A1 variant was higher than the wild type for the patients in the CAD group, there was no significant difference in the polymorphism of UGT1A1 between the patients in the CAD group and the participants in the control group.

The interactome: Predicting the protein-protein interactions in cells

“…Recent advances in System Biology, especially in the context of high-throughput DNA sequencing (genomics), gene expression (transcriptomics), metabolite and ion analysis (metabolomics/ionomics) and protein analysis (proteomics) carries with it the challenge of processing and interpreting the accumulating data sets [90][91][92][93][94][95]. Publicly accessible databases and bioinformatic tools are employed to mine this data in order to filter relevant correlations and create models describing physiological states [90].…”

Serum Bilirubin and Genes Controlling Bilirubin Concentrations as Biomarkers for Cardiovascular Disease

“…The UGT1A1 variants were found to be associated with bilirubin concentrations but not with CAD (47 ). A recent small Chinese case-control study on CAD demonstrated similar results in that the UGT1A1 variants were associated with bilirubin concentrations but not with CAD (75 ). It is also important to note that in 3 of the 5 studies (47,73,74 ), possible underlying liver disease (10,20 ) was not controlled for.…”

Bilirubin in coronary artery disease: Cytotoxic or protective?

“…Hsieh et al[38] sought to explore the association of UGTA1A polymorphisms with risk of CAD development. A case-control design was set up ( n = 135; cases = 61, controls = 74) and although bilirubin levels in the control group were found to be significantly higher than CAD group, no significant differences were observed in the polymorphism of UGTA1A between the two groups.…”