Polymorphisms of the T cell receptor CD3δ and CD3ɛ chains affect anti-CD3 antibody binding and T cell activation

Boding, Lasse; Nielsen, Morten Holtegaard; Bonefeld, Charlotte M.; Essen, Marina Rode von; Nielsen, Bodil; Lauritsen, Jens Peter H.; Hansen, Anker; Nielsen, Morten Milek; Kongsbak, Martin; Rubin, Maria; Vennegaard, Marie T.; Ødum, Niels; Geisler, Carsten

doi:10.1016/j.molimm.2010.06.012

Cited by 2 publications

(2 citation statements)

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“…23 In many studies on TCR signaling, mAb that recognize the invariant ectodomain of CD3ε have been used to activate T cells. 24 Considerable evidence supports the contention that oxidative stress is one of the basic factors in the etiology of numerous diseases, and oxidative stress has been shown to activate extracellular signal-regulated kinases (ERK) 1/2 simultaneously. 25,26 Interestingly, previous results also show that antioxidants (e.g., N-acetyl-L-cysteine, NAC) added before other toxin treatment can rescue cells from cytotoxicity induced by the toxin through decreased oxidative damage.…”

Section: ■ Introductionmentioning

confidence: 99%

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Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Hao

Pan

et al. 2015

J. Agric. Food Chem.

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The aim of the present study is to investigate whether aflatoxin B1 (AFB1)-induced immunotoxicity is associated with oxidative stress and the expression of extracellular regulated protein kinases (ERK) 1/2. The primary splenocytes isolated from healthy pigs were activated and proliferated by anti-pig-CD3 monoclonal antibodies (mAb) in the present experiment, which is an antigen-specific stimulant. Results indicated that cell proliferation and interleukin-2 (IL-2) production were significantly suppressed by AFB1 from 4 to 8 μg/mL in a dose-dependent manner compared to the control group. Furthermore, AFB1 significantly increased malondialdehyde (MDA) levels, decreased reduced glutathione (GSH) and total superoxide dismutase levels, and up-regulated p-ERK1/2 expression in the activated splenocytes. N-Acetyl-l-cysteine blocked anti-CD3-induced T-cell suppression by AFB1 through increasing intracellular concentrations of GSH levels, decreasing MDA levels, and down-regulated p-ERK1/2 expression, respectively. Inhibition of the ERK1/2 expression by ERK-specific iRNA attenuated the decrease of T-cell proliferation and IL-2 production induced by AFB1. It was concluded that AFB1 inhibits anti-CD3-induced lymphocyte proliferation and IL-2 production by the oxidative stress mediated ERK1/2 MAPK signaling pathway.

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Section: ■ Introductionmentioning

confidence: 99%

“…Stimulation of monoclonal antibodies (mAb) is proved to be more specific than mitogen response in some studies . In many studies on TCR signaling, mAb that recognize the invariant ectodomain of CD3ε have been used to activate T cells …”

Section: Introductionmentioning

confidence: 99%

Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Hao

Pan

et al. 2015

J. Agric. Food Chem.

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Dietary protein sources differentially affect microbiota, mTOR activity and transcription of mTOR signaling pathways in the small intestine

et al. 2017

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Dietary protein sources can have profound effects on host-microbe interactions in the gut that are critically important for immune resilience. However more knowledge is needed to assess the impact of different protein sources on gut and animal health. Thirty-six wildtype male C57BL/6J mice of 35 d age (n = 6/group; mean ± SEM body weight 21.9 ± 0.25 g) were randomly assigned to groups fed for four weeks with semi synthetic diets prepared with one of the following protein sources containing (300 g/kg as fed basis): soybean meal (SBM), casein, partially delactosed whey powder, spray dried plasma protein, wheat gluten meal and yellow meal worm. At the end of the experiment, mice were sacrificed to collect ileal tissue to acquire gene expression data, and mammalian (mechanistic) target of rapamycin (mTOR) activity, ileal digesta to study changes in microbiota and serum to measure cytokines and chemokines. By genome-wide transcriptome analysis, we identified fourteen high level regulatory genes that are strongly affected in SBM-fed mice compared to the other experimental groups. They mostly related to the mTOR pathway. In addition, an increased (P < 0.05) concentration of granulocyte colony-stimulating factor was observed in serum of SBM-fed mice compared to other dietary groups. Moreover, by 16S rRNA sequencing, we observed that SBM-fed mice had higher (P < 0.05) abundances of Bacteroidales family S24-7, compared to the other dietary groups. We showed that measurements of genome-wide expression and microbiota composition in the mouse ileum reveal divergent responses to diets containing different protein sources, in particular for a diet based on SBM.

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Polymorphisms of the T cell receptor CD3δ and CD3ɛ chains affect anti-CD3 antibody binding and T cell activation

Cited by 2 publications

References 24 publications

Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Dietary protein sources differentially affect microbiota, mTOR activity and transcription of mTOR signaling pathways in the small intestine

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Polymorphisms of the T cell receptor CD3δ and CD3ɛ chains affect anti-CD3 antibody binding and T cell activation

Cited by 2 publications

References 24 publications

Aflatoxin B1 Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Aflatoxin B1 Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Dietary protein sources differentially affect microbiota, mTOR activity and transcription of mTOR signaling pathways in the small intestine

Contact Info

Product

Resources

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Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway

Aflatoxin B₁ Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway