Polymorphisms of the interleukin-4 gene in Chinese patients with systemic lupus erythematosus in Taiwan

Abstract

We aimed to evaluate the relationship between two polymorphisms of the IL4 gene (-590T/C and intron 3) and systemic lupus erythematosus in Chinese patients in Taiwan. This study included 91 patients with systemic lupus erythematosus (SLE) and 163 unrelated, age matched healthy controls living in the same area. The typing of -590T/C and intron 3 VNTR (variable number of tandem repeats) polymorphisms were performed by PCR-RFLP and PCR, respectively. Allelic frequencies and carriage rates between SLE patients and controls were compared, and the relationship between allelic frequencies and clinical manifestations of SLE was evaluated. The genotype frequencies of IL-4 intron 3 were found to differ significantly between SLE patients with and without discoid rash (chi-square test, P = 0.03 5). The allelic frequency of intron 3 RP1 was significant different in the patients with discoid rash when compared to patients without this clinical feature (OR = 3.70, 95% CI 2.04-6.72, chi2 test, P = 0.029). The RP1/RP1 homozygous carriage was significantly associated with patients with discoid rash when compared to patients without this clinical feature (OR = 6.04, 95% CI 2.81-12.95, P = 0.01). The allelic frequency of -590T was significant different in the patients with discoid rash when compared to patients without this clinical feature (OR = 3.44, 95% CI 1.88-6.31, chi-square test, P=0.04). The T/T homozygous carriage was significantly associated with patients with discoid rash when compared to patients without this clinical feature (OR = 5.41, 95% CI 2.50-11.68, P = 0.02). We describe a novel association between RPI/RPI and T/T homozygous carriage and patients with discoid rash. The role of the intron 3 polymorphism of the IL4 gene in SLE remains unclear and further substantiation based on larger patient samples is needed.

Association of variable number of tandem repeats polymorphism in the IL-4 gene with end-stage renal disease in Malaysian patients

“…A significant association of IL-4 VNTR could influence the type of immune response and increase the development and progression of IgA nephropathy in a Japanese population (Nakashima et al, 2002;Masutani et al, 2003). However, our results were contradictory to findings for other populations (Wu et al, 2003). The discrepancies with previous studies may be due to ethnicity differences, heterogeneous population or sampling bias or possibly due to the environmental factors contributing to the negative associations (Persu, 2006).…”

“…The last ten years have witnessed an increase in the number of patients entering renal replacement therapy due to ESRD (Wu et al, 2003). In Malaysia, the prevalence of dialysis patients increased from 5542 in 1999 to 17,015 in 2007and almost 19,000 in 2008(16th Report of the Malaysian Dialysis & Transplant Registry, 2008.…”

Disease association of the interleukin-18 promoter polymorphisms in Taiwan Chinese systemic lupus erythematosus patients

“…3,[31][32][33][34][35][36][37] In this study, this is the first investigation of the association of IL-18 promoter polymorphisms with The w 2 test (2 Â 2 table) was performed to obtain the P-value. The percentages of SLE and control with ht1 were compared with the percentages of SLE and control without ht1.…”

“…[1][2][3][4][5] Arthritis, serum autoantibodies, renal disorder, oral ulcers, skin rash and photosensitivity are developed in combination with one or more symptoms observed in SLE patients. The association of SLE with autoimmunity and inflammation suggests the importance of genes involved in the cytokine network.…”

Nonassociation of Interleukin 4 Intron 3 and 590 Promoter Polymorphisms with Bronchopulmonary Dysplasia for Ventilated Preterm Infants

“…Although IL-4 had not been present in tracheal-alveolar lavage in the fi rst 3 days of infants who later developed BPD [12] , studies have demonstrated that IL-4 is associated with lung injury, infl ammation cytokines and fi brosis [10,11] . IL-4 polymorphisms are associated with various diseases including systemic lupus erythematosus, minimal change nephrotic syndrome, severe respiratory syncytial virus bronchiolitis [31][32][33] and asthma [34,35] . It is reasonable to speculate that IL-4 polymorphism may be associated with the progression of lung injury in BPD.…”