Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs

Abstract

The genetic factors determining the phenotypic variation of porcine fatness phenotypes are still largely unknown. We investigated whether the polymorphism of eight genes ( MIGA2 , CRY2 , NPAS2 , CIART , ARNTL2 , PER1 , PER2 and PCK1 ), which display differential expression in the skeletal muscle of fasted and fed sows, is associated with the variation of lipid and mRNA expression phenotypes in Duroc pigs. The performance of an association analysis with the GEMMA software demonstrated that the rs330779504 SNP in the MIGA2 gene is associated with LDL concentration at 190 days (LDL 2 , corrected P -value = 0.057). Moreover, the rs320439526 SNP of the CRY2 gene displayed a significant association with stearic acid content in the longissimus dorsi muscle (LD C18:0, corrected P -value = 0.015). Both SNPs were also associated with the mRNA levels of the corresponding genes in the gluteus medius skeletal muscle. From a biological perspective these results are meaningful because MIGA2 protein plays an essential role in mitochondrial fusion, a process tightly connected with the energy status of the cell, while CRY2 is a fundamental component of the circadian clock. However, inclusion of these two SNPs in chromosome-wide association analyses demonstrated that they are not located at the peaks of significance for the two traits under study (LDL 2 for rs330779504 and LD C18:0 for rs320439526), thus implying that these two SNPs do not have causal effects.

Weighted Single-Step GWAS Identified Candidate Genes Associated with Growth Traits in a Duroc Pig Population

“…Hofer et al [ 59 ] found that the silence of PEX6 affects adipocyte differentiation and increases peroxisomal fatty acid oxidation–reduction. For the CRY2 gene, Mármol-Sánchez et al [ 60 ] reported that the polymorphism of CRY2 was significantly associated with stearic acid content in the longissimus dorsi muscle in Duroc pigs. The Acyl-CoA Thioesterase 8 ( ACOT8 ) gene is located in the region of 47.67–48.83 Mb on SSC17.…”

Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

“…In this comparison, control piglets upregulated SESN2 and ANPEP , which participate in stress response and inhibit mTOR-mediated cell growth 35 , and have proteolytic activity 36 , respectively. In turn, they upregulated a key gene in circadian rhythm regulation of energy homeostasis and lipid metabolism 37 , PER2 , as well as ANKRD1 , involved in muscle tissue development 38 and ARG2 , which has been suggested to be an adequate biomarker for feed efficiency 39 . Their counterparts increased the abundance of TNNT1 , which codes for a contractile apparatus protein that has been related to muscle growth 40 .…”

Non-synonymous variation and protein structure of candidate genes associated with selection in farm and wild populations of turbot (Scophthalmus maximus)

“…The circadian-associated transcriptional repressor CIART is involved in the eye regression of cave molly ( Poecilia mexicana ) 114 , whereas turbot ciart proved to be differentially expressed in freshwater- versus seawater-acclimated fish 115 . A missense polymorphism in pig ciart was reported to be associated with backfat thickness 116 . Both the Asn and Ser residues in the polymorphic position 271 of turbot CIART are found in other teleost.…”