Polymorphisms of the bovine DKK2 and their associations with body measurement traits and meat quality traits in Qinchuan cattle

Abstract

The objective of this research were to detect bovine Dickkopf 2 (DKK2) gene polymorphism and analyze their associations with body measurement traits (BMT) and meat quality traits (MQT) of animals. Blood samples were taken from a total of 541 Qinchuan cattle aged from 18 to 24 months. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed to find out DKK2 single-polymorphism nucleotide (SNPs) and to explore their possible association with BMT and MQT. Sequence analysis of DKK2 gene revealed 2 SNPs (C29 T and A169C) in 5' untranslated region (5'UTR) of exon 1.C29T and A164T SNPs are both synonymous mutation, which showed 2 genotypes namely (CC, CT) and (AA and AC), respectively. Association analysis of polymorphism with body measurement and meat quality traits at the two locus showed that there were significant effects on CT, BL, RL, PBW, BFT, LMA, and IFC. These results suggest that the DKK2 gene might have potential effects on BMT and MQT in Qinchuan cattle population and could be used for marker-assisted selection.

Integrated analysis strategy of genome-wide functional gene mining reveals DKK2 gene underlying meat quality in Shaziling synthesized pigs

“…Currently, research on DKK2 primarily focuses on tumors, with several papers reporting abnormal expression of DKK2 in tumor tissues [ 60 – 62 ]. Furthermore, studies have indicated that the SNP (C29T) in exon 1 of the DKK2 is associated with intramuscular fat content in Qinchuan cattle [ 63 ]. In experiments using c3h/10t1/2 mesenchymal stem cells of mice, researchers have verified that the DKK2 can inhibit lipogenesis [ 64 ].…”

Skeletal muscle and liver gene expression profiles in finishing steers supplemented with Amaize

“…PPARGC1A and FOXO1 also induce SESN2 and TRIM63 expression, both of which are important players in controlling lipid metabolism by enhancing mitochondrial biogenesis and shuttling adenosine triphosphate to myofibrils in mice, respectively (Seo, Seo, Ki, & Shin, ; Willis et al., ). DKK2 , another target gene for PPARGC1A and FOXO1 , is associated with intramuscular fat content and backfat thickness in cattle (Zhan, Gao, Huangfu, Fu, & Zan, ). Therefore, the data from the IPA analysis indicating that the adipogenic effects of Amaize as indicated by higher backfat in the Amaize group was likely driven by the upregulation of lipid deposition‐related genes in skeletal muscle, including PPARGC1A , ABRA , FOXO1 , FNIP2 , APOC3 , SESN2 , TRIM63 , and DKK2 .…”

Genome-wide association study and predictive ability for growth traits in Nellore cattle

“…In a study with experimental rat population, the DKK2 gene was related with postnatal development and growth (Bodine et al, 2004). Zhan et al (2015), also associated the expression of this gene with skeletal growth traits as well as meat quality traits in Qinchuan cattle.…”