Polymorphisms of TGF-β1 and TGF-β3 in Chinese women with gestational diabetes mellitus

Xu, Yinglei; Wei, Chunlian; Wu, Cuijiao; Han, Mengmeng; Wang, Jingli; Hou, Hongwei; Zhang, Lu; Liu, Shiguo; Chen, Ying

doi:10.1186/s12884-020-03459-w

Cited by 3 publications

(3 citation statements)

References 46 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, studies have shown that TGF-β can inhibit trophoblast proliferation by activating Smad protein and p38/MAPK pathway, leading to trophoblast dysplasia, which is not conducive to embryo transfer and pregnancy [10]. The results of our bioinformatics suggested that TGFBI and TGFB2, TGF-β-related proteins, were significantly up-regulated in GDM, which was in accordance with recent evidences [13,14].…”

Section: Discussionsupporting

confidence: 88%

Up-regulation of TGFBI and TGFB2 in the plasma of gestational diabetes mellitus patients and its clinical significance

et al. 2021

View full text Add to dashboard Cite

Background Gestational diabetes mellitus (GDM) reflects a deficiency in the relative need for insulin during pregnancy, as well as temporary metabolic stress in the placenta and fetus. Our study aimed to research the potential diagnostic value of transforming growth factor-beta-induced protein ig-h3 (TGFBI) and transforming growth factor beta-2 proprotein (TGFB2) for GDM patients. Methods Online database Gene Expression Omnibus (GEO) was used to screen for different expressed genes (DEGs) associated with GDM. Meanwhile, KEGG and GO were used to analyze the molecular functions as well as pathways of enriched DEGs. One hundred ten pregnant women diagnosed with GDM and 110 healthy controls were enrolled, of whose placenta and fasting venous blood samples were collected. mRNA expression levels were determined by real-time quantitative polymerase chain reaction (RT-qPCR), and fasting blood glucose (FBG) was measured by the clinical lab of hospital. Furthermore, receiver operating characteristics curve (ROC) analysis was performed to evaluate the sensitivity and specificity of detection indexed in the placenta and plasma of GDM patients. Finally, Pearson and Spearman analysis was used for the correlation analysis. Results After GEO data analysis, TGFBI and TGFB2 were identified as the most significantly up-regulated genes of GDM. TGFBI and TGFB2 expressions in placenta and plasma samples of GDM patients were in line with bioinformatic analysis. Meanwhile, the area under the curve (AUC) of TGFBI in the placenta and plasma for the diagnosis of GDM were 0.8783 (95% CI, 0.8281 to 0.9284) and 0.7832 (95% CI, 0.7215 to 0.8449) while for TGFB2 were 0.9225 (95% CI, 0.8829 to 0.9621) and 0.8961 (95% CI, 0.8526 to 0.9396). Besides, levels of TGFBI along with TGFB2 in the placenta were positively correlated with that in the plasma of GDM patients. Furthermore, both TGFBI and TGFB2 expressions in the plasma were positively correlated with FBG levels of the GDM patients. Conclusions TGFBI and TGFB2 were up-regulated in the placenta and plasma of GDM patients, and TGFBI and TGFB2 in the plasma are potent to be diagnostic markers for the GDM. Keywords Biomarker • Different expressed genes (DEGs) • Gestational diabetes mellitus (GDM) • GEO database • Transforming growth factor beta-2 proprotein (TGFB2) • Transforming growth factor-beta-induced protein ig-h3 (TGFBI)

show abstract

Section: Discussionsupporting

confidence: 88%

Up-regulation of TGFBI and TGFB2 in the plasma of gestational diabetes mellitus patients and its clinical significance

et al. 2021

View full text Add to dashboard Cite

show abstract

“…A recent study also showed that growth differentiation factor-15, a novel member of the TGF-β family, was highly expressed in patients with GDM [27]. Xu et al [28] found that the TGF-β3 rs2284792 AA and AG genotypes were significantly increased in patients with GDM among the Chinese population. Therefore, TGF-β and inflammation are likely to be closely related to GDM, although further exploration is needed.…”

Section: Discussionmentioning

confidence: 95%

“…Xu et al. [28] found that the TGF‐β3 rs2284792 AA and AG genotypes were significantly increased in patients with GDM among the Chinese population. Therefore, TGF‐β and inflammation are likely to be closely related to GDM, although further exploration is needed.…”

Section: Discussionmentioning

confidence: 99%

Placental and plasma early predictive biomarkers for gestational diabetes mellitus

Wei

Huang

et al. 2022

Proteomics Clinical Apps

View full text Add to dashboard Cite

Purpose Gestational diabetes mellitus (GDM) is a common disease that can give rise to adverse obstetric outcomes. For successful early intervention, more studies on novel predictive biomarkers for GDM are required. Experimental Design The protein expression profiles of placental tissue from patients with GDM and normal pregnant women were investigated using data‐independent acquisition proteomics with five biological replicates. Early pregnancy maternal plasma samples from the GDM (n = 79) and control (n = 81) groups were used for further validation of candidate biomarkers. Results We identified 37 differentially expressed proteins between the two groups. CD109 antigen (CD109) and endosialin (CD248) were identified as hub proteins. In the validation experiments, CD109 expression was lower in the early pregnancy maternal plasma of patients with GDM compared with that in normal pregnant women, and CD248 expression was higher in the GDM group. The area under the curve of CD109, CD248, and their combination as indicators in early pregnancy maternal plasma was 0.681, 0.609, and 0.695, respectively. Conclusions and Clinical Relevance The present study is the first to obtain preliminary evidence that CD109 and CD248 can predict GDM during early pregnancy, as well as providing proteome‐level insights into this disease's pathological mechanisms.

show abstract

TET2-mediated DNA hydroxymethylation of TGFB1 is related to selective intrauterine growth restriction in monochorionic twin pregnancies

Jiang,

Li,

Zhong

et al. 2023

Placenta

View full text Add to dashboard Cite

Polymorphisms of TGF-β1 and TGF-β3 in Chinese women with gestational diabetes mellitus

Cited by 3 publications

References 46 publications

Up-regulation of TGFBI and TGFB2 in the plasma of gestational diabetes mellitus patients and its clinical significance

Up-regulation of TGFBI and TGFB2 in the plasma of gestational diabetes mellitus patients and its clinical significance

Placental and plasma early predictive biomarkers for gestational diabetes mellitus

TET2-mediated DNA hydroxymethylation of TGFB1 is related to selective intrauterine growth restriction in monochorionic twin pregnancies

Contact Info

Product

Resources

About