Polymorphisms of interleukin 4 and interleukin 4 receptor genes and bronchial asthma risk among Egyptian children

Elsaid, A.; Shoaib, Rasha M. S.; Badr, Sara S.; Wahba, Yahya; Ayyad, Seif‐Eldin N.

doi:10.1016/j.clinbiochem.2021.04.006

Cited by 10 publications

(7 citation statements)

References 45 publications

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“…In the present study, with a higher wild genotypic frequency among cases, no significant association of SNP at position -33CT (rs2070874) of IL-4 was found with the disease. The observations are consistent with universal observations, except for studies in Iran and China [ 18 - 23 ]. While, in the case of genotype frequency for the rs 2243250 polymorphism of IL-4, maximum cases were seen with the heterozygous mutant allele (CT), the homozygous mutant allele (TT) is 1.85 times more risky for disease association than wild-type (CC).…”

Section: Discussionsupporting

confidence: 90%

“…Inducing heavy chain isotype switching, IgE synthesis by B-cells, and acting as a growth factor for Th2 cells, the cytokine IL-4 plays a vital role in the etiology and progression of allergic inflammation and atopy [ 24 ]. Several studies worldwide reported contradictory observations regarding SNP -589/C-590 T's association with asthma [ 8 , 10 , 18 , 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of IL-4 and IL-13 Single Nucleotide Polymorphisms and Their Association With Childhood Asthma and Its Severity: A Hospital-Based Case-Control Study

Deka,

Siddique,

Ahmed

et al. 2024

Cureus

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Background and objectives: Asthma is a common, chronic, atopic respiratory disease that is on the rise among children and adults worldwide. Various environmental, genetic, and biological interactions contribute to the surge in susceptibility to this disease. Interleukin (IL) genes, particularly IL-4 and IL-13, have been linked to asthma pathogenesis. The present study aims to investigate the genetic aberrations, specifically single nucleotide polymorphisms (SNPs) of IL-4 and IL-13, and their association with childhood asthma and its severity. Methods: An unmatched hospital-based case-control study was conducted in a tertiary care hospital in Assam, India. The sample size was calculated to be 120 (60 cases and 60 controls) using the Epi Info software version 7.2 (Centers for Disease Control and Prevention, Atlanta, GA, USA), assuming a confidence interval of 95%, a power of the study at 80%, a ratio of control to cases as 1, a proportion of controls with exposure at 22%, and a proportion of cases with exposure at 46%. A total of 53 clinically diagnosed cases of childhood asthma in the age range of three to 12 years and 39 healthy controls free from respiratory diseases and having no history of asthma and/or allergy of the same age group attending a tertiary care hospital were included in the study. Children who never had asthma or allergies and who did not suffer from any upper or lower respiratory infections for the previous four weeks were considered controls. Prior informed consent and ethical clearance were obtained. Very seriously ill cases and controls were excluded from the study. The genetic investigation used polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP), to discover SNPs in the IL-4 and IL-13 genes. Sequencing analysis was done for the cases with +2044 G>A of the IL-13 gene in relation to the severity of the disease. The difference in the proportions of specific SNPs between cases and controls was analyzed using the χ2 test (a p-value of <0.05 was considered significant). Results. Both the rs2070874 and rs2243250 polymorphisms of IL-4 showed no statistically significant associations. The mutation of the IL-13 gene in 1111C>T was higher among cases than controls. Both genotypic and allelic distributions of the +2044G>A polymorphism of the IL-13 gene revealed a significant association (p<0.05) with the severity of the disease. Conclusion: Genetic aberrations in SNPs of IL-4 and IL-13 are prevalent among the pediatric patients of the study region. The SNP +2044G>A of IL-13 is instrumental in disease manifestation and severity among the pediatric population of the study region.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Evaluation of IL-4 and IL-13 Single Nucleotide Polymorphisms and Their Association With Childhood Asthma and Its Severity: A Hospital-Based Case-Control Study

Deka,

Siddique,

Ahmed

et al. 2024

Cureus

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“…Allergen interacts with IgE and starts inflammation-cascade leading to pro-inflammatory mediators’ flow that takes part in the acute and chronic symptoms of asthma 42 . Regardless of the inaccuracy of serum total IgE in the diagnosis of allergic diseases, modern research has established that it can aid in characterizing refractory asthma phenotypes and configuration of add-on therapies, like anti-IgE monoclonal antibodies, for cases suffering from moderate to severe persistent, uncontrolled asthma 43 . There was no evidence in our study into the association between MUC7 and serum IgE levels in asthmatic individuals.…”

Section: Discussionmentioning

confidence: 99%

MUC7 VNTR polymorphism and association with bronchial asthma in Egyptian children

Saad

Elsaid

Shoaib

et al. 2022

Sci Rep

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Overproduction of mucins in the airways donates largely to airway blockage in asthma patients. Glycoprotein MUC7 plays a role in the clearance of bacteria and has anti-candidacidal criteria. Our goal was to investigate the association between the MUC7 variable number of tandem repeats (VNTR) polymorphism and bronchial asthma among Egyptian children. The MUC7 VNTR polymorphism was investigated among 100 children with bronchial asthma and 100 healthy controls using polymerase chain reaction (PCR) method. Serum levels of immunoglobulin E (IgE), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta1 (TGF-β1) were assessed by enzyme-linked immunosorbent assay (ELISA) technique. The frequencies of 6*5 genotype, 5*5 genotype, (6*5 + 5*5) genotypes, and MUC7*5 allele of the MUC7 VNTR variant were significantly lower among asthmatic patients than controls (p < 0.015, OR = 0.39, 95% CI = 0.19–0.81; p = 0.03, OR = 0.18, 95% CI = 0.04–0.86; p < 0.001, OR = 0.29, 95% CI = 0.15–0.58; p < 0.001, OR = 0.3, 95% CI = 0.17–0.55, respectively). The (6*5 + 5*5) genotypes of the MUC7 VNTR variant were not associated with the clinical manifestations and serum levels of IgE, TNF-α, and TGF-β1 among asthmatic patients (p ˃ 0.05). In conclusion, the (6*5 + 5*5) genotypes of the MUC7 VNTR variant may have a protective role for bronchial asthma in Egyptian children.

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“…Figure 1 displays the study selection flowchart. Collectively, 12 case-control studies, involving 1553 patients and 1904 controls and conforming to the eligibility criteria, were enrolled [12][13][14][15][16][17][18][19][20][21][22][23]. Genotype distributions in each study conformed to HWE, with an exception of the study by Zhang et al Every study was regarded as a separate dataset for the pooled analysis.…”

Section: Study Featuresmentioning

confidence: 99%

Association of the IL-4R Q576R polymorphism with asthma: A meta-analysis

Zhang

Huang

et al. 2022

Arch Med Sci

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IntroductionSeveral articles have examined the relationship of IL-4 receptor gene (IL-4R) polymorphisms with asthma, yet the results remain inconsistent. This may be attributed to the small sample sizes and polymorphism’s small effect size that fail to offer potent statistical power. Therefore, this meta-analysis on related studies was aimed at analyzing how IL-4R Q576R polymorphisms affected the asthma susceptibility.Material and methodsDatabases including Web of Science, PubMed, Google Scholar, CNKI and EMBASE were systematically searched for retrieving pertinent studies. Odds ratios (ORs) and the relevant 95% confidence intervals (CIs) were also calculated.ResultsCollectively, 12 eligible articles involving 1553 patients and 1904 normal subjects were subjected to analysis. There was no distinct relationship between IL-4R Q576R polymorphism and asthma susceptibility, in the entire study population (R vs Q: OR = 1.25, 95% CI = 0.98–1.59; RQ vs QQ: OR = 1.21, 95% CI = 0.98–1.49; RR vs QQ: OR = 1.47, 95% CI = 0.80–2.71; the recessive model: OR = 1.38, 95% CI = 0.80–2.40; the dominant model: OR = 1.25, 95% CI = 0.98–1.60). Subgroup analysis, conducted based on ethnicity and study population, revealed no obvious interrelation.ConclusionsOur findings demonstrated no relationship between IL-4R Q576R polymorphism and asthma susceptibility. More well-designed and large-scale studies are needed to validate the above findings.

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Polymorphisms of interleukin 4 and interleukin 4 receptor genes and bronchial asthma risk among Egyptian children

Cited by 10 publications

References 45 publications

Evaluation of IL-4 and IL-13 Single Nucleotide Polymorphisms and Their Association With Childhood Asthma and Its Severity: A Hospital-Based Case-Control Study

Evaluation of IL-4 and IL-13 Single Nucleotide Polymorphisms and Their Association With Childhood Asthma and Its Severity: A Hospital-Based Case-Control Study

MUC7 VNTR polymorphism and association with bronchial asthma in Egyptian children

Association of the IL-4R Q576R polymorphism with asthma: A meta-analysis

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