Polymorphisms of human cytomegalovirus UL148A, UL148B, UL148C, UL148D genes in clinical strains

Yao, Jian; Sun, Zheng Rong; Ruan, Qiang; Guo, Jin Jin; He, Rong; Yan, Ping; Mao, Zhi; Huang, Yu

doi:10.1016/j.jcv.2006.09.007

Cited by 9 publications

(6 citation statements)

References 23 publications

(18 reference statements)

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“…Although some regions have been sequenced in multiple studies (for example, UL55 (gB)), for the purposes of this figure, we show the data from the study that sequenced the largest region. The data used to construct this figure are from [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [15], [16], which are listed in Text S1.…”

Section: Supporting Informationmentioning

confidence: 99%

“…The genome of AD169 as compared to low passage strains has an approximately 15 kb deletion which encodes an additional 19 or 22 open ORFs, referred to as the UL/b' region [6], [7], [8]. Approximately 20 ORFs of HCMV have been shown to exhibit nucleotide variability when sequenced from infected hosts [9], [10], [11], [12], [13], [14], [15], [16], [17], [18], [19]. These studies have often focused on the variability of ORFs encoding envelope glycoproteins or ORFs of UL/b', which are thought to be important for pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants

et al. 2011

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Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus.

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Section: Supporting Informationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants

et al. 2011

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“…HCMV has approximately 249 kb double‐stranded DNA genome and at least 200 open reading sequences. A high nucleotide variability has been reported in approximately 20 open reading sequences of the HCMV that encode envelope glycoproteins, such as glycoproteins B (UL55) and N (UL73), as well as the UL144 gene, which has an amino acid sequence similar to that of the tumor necrosis factors …”

Section: Introductionmentioning

confidence: 99%

Is the mixture of human cytomegalovirus genotypes frequent in infants with congenital infection at birth in a high seroprevalence population?

Figueiredo

Marques

Mussi-Pinhata

et al. 2018

Journal of Medical Virology

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It is still not well known, in a population with high human cytomegalovirus (HCMV) seroprevalence, whether a child with congenital infection harbors multiple viral strains at birth, and whether the prolonged viral excretion in these children is secondary to the persistence of the same viral strain. To verify the genomic diversity of HCMV detected in congenitally infected children, the nucleotide viral sequences from urine and/or saliva obtained at birth from 14 newborns with congenital infection and breast milk obtained from mothers of 5 of these children were analyzed. Among the 14 children, 10 had sequential samples until the median age of 10 months. The viral nucleotide sequences in the breast milk were compared with those identified in the respective children at birth. The differentiation of viral strains was based on the variability of 3 regions of viral genes (UL55/gB, UL144, and UL73/gN). In 13/14 children (92.8%), a single genotype was observed at birth. Different viral genotypes were found in 1 child (7.2%). Among the sequential samples from 10 children, the same genotype obtained at birth was detected in 9/10 (90%), and in 1 of them (10%), a genotype change in the urine was found. More than 1 HCMV strain in milk was observed in 2 mothers (2/5, 40%). In a population with high seroprevalence, a single genotype was found in the majority of infected children. Reinfection did not frequently occur in the first months of life. Maternal reinfection does not seem to be a rare event in transmitter mothers.

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“…Nucleotide variability was determined for approximately 20 open reading frames (ORFs) of HCMV that encode, e.g., viral envelope glycoproteins B (gB, UL55 ), H (gH, UL75 ), and N (gN, UL73 ), as well as chemokines and chemokine receptors, like a tumor necrosis factor (TNF)-α receptor (pUL144, UL144 ) [6–9]. Although sequence variability in the large genome generates extensive viral strain diversity, the clinical significance of HCMV genotypes remains rather unknown.…”

Section: Introductionmentioning

confidence: 99%

Mixed infections with distinct cytomegalovirus glycoprotein B genotypes in Polish pregnant women, fetuses, and newborns

Rycel

Wujcicka

Zawilińska

et al. 2014

Eur J Clin Microbiol Infect Dis

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The purpose of this investigation was to describe a distribution of cytomegalovirus (CMV) single and multiple genotypes among infected pregnant women, their fetuses, and newborns coming from Central Poland, as well as congenital cytomegaly outcome. The study involved 278 CMV-seropositive pregnant women, of whom 192 were tested for viral DNAemia. Human cytomegalovirus (HCMV) genotyping was performed for 18 of 34 pregnant women carrying the viral DNA and for 12 of their 15 offspring with confirmed HCMV infections. Anti-HCMV antibodies levels were assessed by chemiluminescence immunoassay (CLIA) and enzyme-linked fluorescence assay (ELFA) tests. Viral DNA loads and genotypes were determined by real-time polymerase chain reaction (PCR) assays for the UL55 gene. In the pregnant women, we identified HCMV gB1, gB2, gB3, and gB4 genotypes. Single gB2, gB3, or gB4 genotypes were observed in 14 (77.8 %) women, while multiple gB1–gB2 or gB2–gB3 genotypes were observed in four (22.2 %). Maternal HCMV genotypes determined the genotypes identified in their fetuses and newborns (p ≤ 0.050). Half of them were infected with single HCMV gB1, gB2, or gB3 genotypes and the other half with multiple gB1–gB2 or gB2–gB3 genotypes. Single and multiple genotypes were observed in both asymptomatic and symptomatic congenital cytomegaly, although no gB3 genotype was identified among asymptomatic cases. In Central Poland, infections with single and multiple HCMV strains occur in pregnant women, as well as in their fetuses and neonates, with both asymptomatic and symptomatic infections. HCMV infections identified in mothers seem to be associated with the viral genotypes in their children.

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Polymorphisms of human cytomegalovirus UL148A, UL148B, UL148C, UL148D genes in clinical strains

Cited by 9 publications

References 23 publications

Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants

Extensive Genome-Wide Variability of Human Cytomegalovirus in Congenitally Infected Infants

Is the mixture of human cytomegalovirus genotypes frequent in infants with congenital infection at birth in a high seroprevalence population?

Mixed infections with distinct cytomegalovirus glycoprotein B genotypes in Polish pregnant women, fetuses, and newborns

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