Polymorphisms of genes coding for ghrelin and its receptor in relation to colorectal cancer risk: a two-step gene-wide case-control study

Campa, Daniele; Pardini, Barbara; Naccarati, Alessio; Vodičková, Ľudmila; Novotný, Jan; Steinke, Verena; Rahner, Nils; Holinski‐Feder, Elke; Morak, Monika; Schackert, Hans K.; Görgens, Heike; Kötting, Judith; Betz, Beate; Kloor, Matthias; Engel, Christoph; Büttner, Reinhard; Propping, Peter; Försti, Asta; Hemminki, Kari; Barale, Roberto; Vodička, Pavel; Canzian, Federico

doi:10.1186/1471-230x-10-112

Cited by 24 publications

(23 citation statements)

References 39 publications

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“…Genetic polymorphisms in the GHRL gene may affect GHRL production or protein expression, thereby modulating disease risk. Several studies have reported that GHRL polymorphisms might be associated with obesity [ 25 ], metabolic syndrome [ 27 ], type 2 diabetes [ 29 ], breast cancer [ 30 ], and colorectal cancer [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have investigated the association between the GHRL rs26311, rs27647, rs696217, and rs34911341 polymorphisms with the risk of various diseases such as obesity [ 25 ], metabolic syndrome [ 27 , 28 ], type 2 diabetes [ 29 ], breast cancer [ 30 ], and colorectal cancer [ 31 ]. Until now, there were only two studies that examined the association between GHRL polymorphisms and the risk of liver diseases [ 17 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Association of Ghrelin Gene Polymorphisms and Serum Ghrelin Levels with the Risk of Hepatitis B Virus-Related Liver Diseases in a Chinese Population

et al. 2015

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BackgroundThe functions of ghrelin (GHRL) include anti-inflammatory effects, reduction of the fibrogenic response, protection of liver tissue, and regulation of cell proliferation. Genetic variations in the GHRL gene may play an important role in the development of chronic hepatitis B (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Therefore, we investigated whether GHRL gene polymorphisms and its serum levels are associated with hepatitis B virus (HBV)-related diseases risk in a Chinese population.Methods176 patients with CHB, 106 patients with HBV-related LC, 151 patients with HBV-related HCC, and 167 healthy controls were recruited in the study. Genotyping of GHRL rs26311, rs27647, rs696217, and rs34911341 polymorphisms were determined with the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing. The serum GHRL concentrations were determined using enzyme-linked immunosorbent assay (ELISA).ResultsBinary logistic regression analyses adjusting for gender and age revealed that a significant increased risk of LC was found in the GHRL rs26311 GC genotype and combined GC+CC genotypes when compared with the GG genotype (GC vs. GG: OR = 1.671, 95% CI = 1.013–2.757, P = 0.044; GC+CC vs. GG: OR = 1.674, 95% CI = 1.040–2.696, P = 0.034). In subgroup analysis by gender, binary logistic regression analyses adjusting for age showed that the GHRL rs26311 C allele and combined GC+CC genotypes were associated with a significantly increased risk to LC in males (C vs. G OR = 1.416, 95% CI = 1.017–1.972, P = 0.040; GC+CC vs. GG: OR = 1.729, 95% CI = 1.019–2.933, P = 0.042). In addition, we found significant decreased serum GHRL levels in LC patients compared with the healthy controls. However, there was no significant association of the GHRL rs26311 polymorphism with serum GHRL levels in LC patients.ConclusionsThese observations suggest that the GHRL rs26311 polymorphism is associated with an increased risk to HBV-related LC, especially in men. We also found an inverse association of serum GHRL levels with LC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of Ghrelin Gene Polymorphisms and Serum Ghrelin Levels with the Risk of Hepatitis B Virus-Related Liver Diseases in a Chinese Population

et al. 2015

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“…A total of 17 Chironomid samples (6, 7, and 4 samples obtained from areas I, II, and III, respectively) were genotyped using the Illumina BovineHD Genotyping BeadChip system (Neogen, Lincoln NE, USA), which contained 419 431 SNPs. DNA was isolated from the organisms using a standard proteinase K digestion followed by phenol/chloroform extraction and ethanol precipitation (Campa et al 2010), and then the DNA concentrations were measured using a Nano-Drop2000 spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). The integrity of the genomic DNA was confirmed by means of 1% agarose gel electrophoresis.…”

Section: Fieldwork and Samplingmentioning

confidence: 99%

Framework of multidimensional macrobenthos biodiversity to evaluate ecological restoration in wetlands

Yang

Sun

et al. 2019

Environ. Res. Lett.

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Sustaining biodiversity is a priority for ecological restoration in degraded wetlands, and biodiversity is an inherently multidimensional ecosystem property, as it includes genetic, taxonomic, functional, and other types of diversity. However, most restoration projects have only employed taxonomic diversity to assess restoration, and given the multidimensional nature of biodiversity, this may partially describe the true situation. In this paper, we constructed a multidimensional macrobenthos biodiversity framework (for genetic, taxonomic, and functional diversity), developed a holistic biodiversity index that accounted for these dimensions, and explored the consequences of freshwater restoration in a case study of this index. We found that freshwater restoration increased genetic and taxonomic diversity, but had no significant effect on functional diversity, and that the functional traits of the macrobenthos appeared to have converged on homogeneous functional traits. On the other hand, freshwater restoration had a significant positive effect on the holistic biodiversity. The relationship between ecosystem function (in macrobenthos biomass) and holistic biodiversity in the restoration areas was unimodal, with the highest biomass found at intermediate levels of holistic biodiversity. The multidimensional framework challenged the utility of using any one dimension of biodiversity as a surrogate for other dimensions. The holistic biodiversity index provided more comprehensive and holistic insights into the management of freshwater restoration projects.

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“…In human, GHSR gene polymorphism was reported to have an association with eating behavior (Gueorguiev et al 2009), obesity and glucose metabolism (Mager et al 2008), and colorectal cancer risk (Campa et al 2010). Studies of GHSR gene have been reported in other animals, such as cattle (Komatsu et al 2015), duck (Nie et al 2009), quail (Kitazawa et al 2009), and goldfish (Sánchez-Bretaño et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The Ghrelin Receptor (Ghsr) Gene Polymorphism in Indonesian Local Chicken and Crossbreed Is Associated With Carcass Traits

Khaerunnisa

Jakaria

Arief

et al. 2018

anprod

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Ghrelin receptor (GHSR) gene is candidate gene for growth performance in chicken by modulating growth hormone release from the pituitary by binding to its ligand of ghrelin. Ghrelin gene, or growth hormone secretagogue (GHS) gene, is well known as feed intake and energy homeostasis regulator in mammals and birds. The objectives of this study were to identify the polymorphism of the T1857C GHSR locus in Indonesian local chicken and to evaluate its effects on carcass traits. The gene polymorphism was identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Hin6I restriction enzyme. Effect of genotype on carcass composition was analyzed using SAS General Linear Model (GLM) procedure. The genotyping was performed on 343 individuals including Merawang, Pelung, Sentul, Kampung, broiler (strain Cobb, parent stock), F1 crossbred of Kampung x broiler (strain Cobb, parent stock), and F2 crossbred of Kampung x broiler (strain Cobb, parent stock). All individuals were successfully amplified and were resulted in a 470 bp PCR product. This locus was polymorphic with two alleles (T and C) and three genotypes (TT, CT, and CC). The T allele and TT genotype were predominant in all populations. Individuals with CT genotype were significantly had higher live weight at 26w, carcass weight, commercial cuts weights, and muscles weights than TT genotype in F2 crossbred of Kampung x broiler population. Association of the T1857C GHSR locus-polymorphism with chicken carcass composition has been described in Indonesian chicken, providing evidence that GHSR might be an important candidate gene for chicken carcass traits.

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Polymorphisms of genes coding for ghrelin and its receptor in relation to colorectal cancer risk: a two-step gene-wide case-control study

Cited by 24 publications

References 39 publications

Association of Ghrelin Gene Polymorphisms and Serum Ghrelin Levels with the Risk of Hepatitis B Virus-Related Liver Diseases in a Chinese Population

Association of Ghrelin Gene Polymorphisms and Serum Ghrelin Levels with the Risk of Hepatitis B Virus-Related Liver Diseases in a Chinese Population

Framework of multidimensional macrobenthos biodiversity to evaluate ecological restoration in wetlands

The Ghrelin Receptor (Ghsr) Gene Polymorphism in Indonesian Local Chicken and Crossbreed Is Associated With Carcass Traits

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