Polymorphisms of CCSER1 Gene and Their Correlation with Milk Quality Traits in Gannan Yak (Bos grunniens)

Abstract

Coiled-coil serine-rich protein 1 (CCSER 1) gene is a regulatory protein gene. This gene has been reported to be associated with various economic traits in large mammals in recent years. The aim of this study was to investigate the association between CCSER1 gene single nucleotide polymorphisms (SNPs) and Gannan yaks and to identify potential molecular marker loci for breeding milk quality in Gannan yaks. We genotyped 172 Gannan yaks using Illumina Yak cGPS 7K liquid microarrays and analyzed the correlation between the three SNPs loci of the CCSER1 gene and the milk qualities of Gannan yaks, including milk fat, protein and casein. It was found that mutations at the g.183,843A>G, g.222,717C>G and g.388,723G>T loci all affected the fat, protein, casein and lactose traits of Gannan yak milk to varying extents, and that the milk quality of individuals with mutant phenotypes was significantly improved. Among them, the milk fat content of AG heterozygous genotype population at g.183,843A>G locus was significantly higher than that of AA and GG genotype populations (p < 0.05); the casein and protein content of mutant GG and CG genotype populations at g.222,717C>G locus was significantly higher than that of wild-type CC genotype population (p < 0.05); and the g.388,723G>T locus of the casein and protein contents of the mutant TT genotype population were significantly higher (p < 0.05) than those of the wild-type GG genotype population. These results provide potential molecular marker sites for Gannan yak breeding.

Polymorphisms within the PRKG1 Gene of Gannan Yaks and Their Association with Milk Quality Characteristics

“…Ear tissue samples were collected from 172 Gannan yaks and stored in liquid nitrogen before being transported back to the laboratory for storage at −80 °C. DNA was extracted from the ear tissue samples following the instructions of the Magnetic Animal Tissue Genomic DNA Kit (DP341, Tiangen Biochemical Technology Co., Ltd., Beijing, China) [ 24 ]. The extracted samples were left to stand on a magnetic rack for 2 min, and after the magnetic beads were completely adsorbed, the DNA solution was transferred to a new centrifuge tube and stored under appropriate conditions.…”

Association between Single Nucleotide Polymorphisms of PRKD1 and KCNQ3 Gene and Milk Quality Traits in Gannan Yak (Bos grunniens)

“…In the yak, PRKD1 gene is located on chromosome 17, and the correlation between PRKD1 gene polymorphism and milk quality in yak has not been mentioned. Previously, we found that three SNPS in the CCSER1 gene were associated with milk quality (milk fat, protein, and casein) in Gannan yaks [ 19 ]. Regions reported to affect milk quality traits in dairy cows are concentrated on chromosomes 1, 6, 11, 13, 14, and 18 [ 20 ].…”