Polymorphisms in the vascular endothelial growth factor (VEGF) gene associated with asthma

Lu, Hui-shan; Zhao, Guifeng; Fu, Mao

doi:10.4238/gmr.15027880

Cited by 10 publications

(8 citation statements)

References 19 publications

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“…These studies utilize a number of techniques that include, but are not limited to, agarose gel electrophoresis, restriction fragment length polymorphism (RFLP), real time/polymerase chain reaction (RT/PCR), Sanger-sequencing and microarrays. Whole blood is the primary source of genomic DNA in most of the clinical investigations associated with genetics, genomics, pharmacogenomics, genetic diseases and epidemiology (Gao et al, 2015, Lu et al, 2016, Rzehak et al, 2016, Visvikis et al, 1998). Buccal swab or saliva is also used to extract DNA for a number of PCR based studies (Küchler et al, 2012, Ng et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method

Qamar

Khan

Arafah

2017

Saudi Journal of Biological Sciences

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In case of studies associated with human genetics, genomics, and pharmacogenetics the genomic DNA is extracted from the buccal cells, whole blood etc. Several methods are exploited by the researchers to extract DNA from the whole blood. One of these methods, which utilizes cell lysis and proteolytic properties of sodium dodecyl sulfate (SDS) and proteinase K respectively, might also be called SDS-PK method. It does not include any hazardous chemicals such as phenol or chloroform and is inexpensive. However, several researchers report the same method with different formulas and conditions. During our experiments with whole blood DNA extraction we experienced problems such as protein contamination, DNA purity and yield when followed some SDS-PK protocols reported elsewhere. A260/A280 and A260/A230 ratios along with PCR amplification give a clear idea about the procedure that was followed to extract the DNA. In an effort to increase the DNA purity from human whole blood, we pointed out some steps of the protocol that play a crucial role in determining the extraction of high quality DNA.

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Section: Introductionmentioning

confidence: 99%

Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method

Qamar

Khan

Arafah

2017

Saudi Journal of Biological Sciences

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“…One study showed that the diagnosis of asthma was positively associated with T alleles in the rs3025020 and rs3025039 single-nucleotide polymorphisms (SNPs) of the VEGF gene in the Chinese population [27]. There is also a report suggesting a borderline association between the G allele of the VEGF gene polymorphism at position -634 G/C and diagnosis of asthma in Tunisian children.…”

Section: Resultsmentioning

confidence: 99%

The –2549 –2567 del18 Polymorphism in VEGF and Irreversible Bronchoconstriction in Asthmatics

Gomułka

Liebhart

Jaskuła

et al. 2019

J Investig Allergol Clin Immunol

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Background: While the importance of vascular endothelial growth factor (VEGF) in the pathogenesis of several diseases (eg, neoplasms) has been proven, its role in asthma, especially in terms of the potential associations between genetic variants of VEGF and airway remodeling, has received relatively little attention. Objectives: This study aimed to evaluate the possible connection between a genetic factor, ie, the polymorphism del/ins in the VEGF promoter region, and airway remodeling potential in asthmatics with and without irreversible bronchoconstriction. Materials and Methods: The study population comprised 82 patients with asthma (of whom 42 had irreversible bronchoconstriction) and a group of 40 controls. DNA was isolated from peripheral blood leukocytes. Polymerase chain reaction was used to type the VEGF (18-bp deletion/insertion) gene polymorphism at loci -2549 -2567. Other factors (ie, smoking, disease duration) were also taken into consideration. Results: The del/del genotype was found in 74.39% of patients with asthma (P=.031; OR=2.38), 80.95% of patients with irreversible bronchoconstriction (P=.012; OR=3.48), and 67.5% patients with reversible bronchoconstriction (P=.251; OR=1.70). The proportion of smokers to nonsmokers was higher (P=.032) and disease duration was longer (P=.041) in patients with irreversible bronchoconstriction than in those with reversible bronchoconstriction. Conclusions: Our results showed that the risk of irreversible bronchoconstriction in asthmatics was associated with the presence of the del18 genotype at the -2549 -2567 position in the promoter region of the VEGF gene, as were disease duration and other factors such as smoking. ResumenAntecedentes: Aunque se ha demostrado la importancia del factor de crecimiento endotelial vascular (VEGF) en la patogénesis de varias enfermedades (p. ej. neoplasias), los datos relativos al asma son escasos, especialmente los relacionados con las posibles asociaciones entre las variantes genéticas de VEGF y la remodelación de las vías respiratorias. Objetivos: En este estudio se propuso evaluar la posible relación entre un factor genético como el polimorfismo del/ins en la región promotora de VEGF y el potencial de remodelación de las vías aéreas en los asmáticos con y sin broncoconstricción irreversible. Materiales y métodos: en el estudio participaron 82 pacientes con asma (42 pacientes con broncoconstricción irreversible) y un grupo de 40 controles. El ADN fue extraído de leucocitos de sangre periférica. Para la tipificación del polimorfismo del gen VEGF (delección / inserción de 18 pb) en loci -2549 -2567 se utilizó el método de reacción en cadena de la polimerasa (PCR). Se consideraron también otros factores (fumar, duración de la enfermedad). Resultados: El genotipo del/del se encontró en el 74,39% de pacientes con asma (p = 0,031; OR = 2,38), el 80,95% de los pacientes con broncoconstricción irreversible (p = 0,012; OR = 3,48) y el 67,5% de los pacientes con broncoconstricción reversible (p = 0,251; OR = 1,70). La proporción de fumado...

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“…VEGF, an important regulator of angiogenesis and vascular remodelling, belongs to the platelet-derived growth factor family and is expressed in tissues rich in blood vessels, such as the lung tissue, and in inflammatory cells, including neutrophils, lymphocytes, monocytes and eosinophils (Lu et al, 2016). VEGF family members include VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, VEGF-F and placental growth factor (PIGF) (Melincovici et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Association of VEGFA polymorphisms with chronic obstructive pulmonary disease in Chinese Han and Mongolian populations

Gao

Wang

Jiao

et al. 2021

Experimental Physiology

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We attempted to define the associations between single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor A (VEGFA) gene and chronic obstructive pulmonary disease (COPD) in Chinese Han and Mongolian cohorts. Five SNPs were genotyped in cohorts of 684 COPD patients (350 Mongolian and 334 Han) and 784 healthy controls (350 Mongolian and 434 Han) using SNPscan multiplex PCR. SNP frequencies, genetic models and haplotypes were analysed using the chi-square test.The associations of SNPs with COPD and linkage disequilibrium were analysed using logistic regression and HaploView, respectively. We found that only rs833068G>A, rs833070T>C and rs3024997G>A were significantly associated with the risk of COPD in the Mongolian population (rs833068: P < 0.

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Polymorphisms in the vascular endothelial growth factor (VEGF) gene associated with asthma

Cited by 10 publications

References 19 publications

Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method

Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method

The –2549 –2567 del18 Polymorphism in VEGF and Irreversible Bronchoconstriction in Asthmatics

Association of VEGFA polymorphisms with chronic obstructive pulmonary disease in Chinese Han and Mongolian populations

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