Polymorphisms in the TNFA promoter region is not associated with palmoplantar pustulosis

Abstract

Polymorphisms of the 5'-flanking promoter/enhancer region of the TNAFA gene were determined in 80 Japanese patients with pulmoplantar pustulosis (PPP). The 5'-flanking region of the TNFA gene from -1107 to 66 was amplified by polymerase chain reaction (PCR) method. Nucleotide sequencing data from the PCR products revealed that 5 single nucleotide polymorphisms at position 1031, -863, -857, -307 and -237. None of the nucleotide substitutions were significantly increased in PPP patients when compared with those in controls. To clarify the linkage among the neighboring genetic marker, we analyzed the association between the polymorphisms in the TNFA promoter region and the NcoI polymorphism in the first intron of the TNFB gene as well as HLA-DR9. The genotype at 1031C is strongly associated with TNFB1 and negatively associated with TNFB2 which is reported to be associated with PPP. These data indicate that TNFA gene centromeric to TNFB is not associated with PPP and the susceptible gene of PPP is located between TNFB and HLA-B.

Association of TNF‐β polymorphism with disease severity among patients infected with hepatitis C virus

“…A complication in the interpretation of the genetic data such as the association of the Nco I polymorphism in the TNF-b gene results from a strong linkage disequilibrum well known to occur in the MHC locus [Niizeki et al, 2000]. It is possible that linkage disequilibrium among genetic markers in the TNF and MHC genes can explain the disease association.…”

Palmoplantar subcorneal pustular dermatosis following adalimumab therapy for rheumatoid arthritis

“…Explanation of the cause of TNF‐α inhibitor‐associated psoriasis would likely focus on shared pathomechanisms with this skin disease, which is much commoner in women and smokers. PPP histologically demonstrates more mast cells and eosinophils than psoriasis vulgaris and has different genetic polymorphisms . TNF‐α expression in palmar eccrine sweat glands in PPP is reduced, possibly indicating a disturbance in the control of this cytokine in acral skin …”

“…PPP histologically demonstrates more mast cells and eosinophils than psoriasis vulgaris and has different genetic polymorphisms. 13,15 TNF-a expression in palmar eccrine sweat glands in PPP is reduced, possibly indicating a disturbance in the control of this cytokine in acral skin. 16 It is acknowledged that clinical and histopathological distinction between pustular psoriasis and SPD can be very difficult.…”

Dermatoses neutrofílicas: parte II

“…65 No association with the TNFA gene was found. 66 Variations in the genes that determine interleukin 19 (IL-19) subfamily of cytokines could influence susceptibility to palmoplantar pustulosis. 67 Smoking is considered a triggering and aggravating factor of PPP.…”

“…63 -PPP has been associated with tumor necrosis factor B (TNFB) gene polymorphism and psoriasis, with TNFA gene polymorphism. 66 -Involvement of the sternoclavicular articulation is more common in PPP 87 -In PPP, articular involvement is usually monoor oligoarticular, whereas in psoriasis it is polyarticular 87 .…”