Polymorphisms in Several Porcine Genes are Associated with Growth Traits

Lyubov, Getmantseva; Kolosov, Anatoly; Леонова, М. А.; Bakoev, Siroj; Klimenko, A.; Vasilenko, V. N.; Radyuk, A. V.

doi:10.3844/ajavsp.2016.136.141

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…In the PIT-1 gene, we demonstrated that genotype AA predominated genotype BB, with allele A was 60% and allele B was 40% in the population. Our results provide strong support for other previous research, such as Lyubov et al (2016), identified the significant effect of genotype Polymorphisms of Candidate Genes Associated with Growth and Carcass Traits in Canadian Duroc Pigs AA/PIT-1 on ADG and LD (Lyubov et al, 2016). Moreover, in European W ild Boar, Pietrain and Meishan pigs, genotype AA was also recorded with a close association with performance and carcass traits (Brunsch et al, 2002).…”

Section: Association Between Mc4r Pit-1 and Studied Traitssupporting

confidence: 91%

Polymorphisms of Candidate Genes Associated with Growth and Carcass Traits in Canadian Duroc Pigs

Tinh

Hợp

Anh

et al. 2021

IJAR

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Background: Selection based on traits such as average daily gain (ADG) and carcass quality in commercial pig populations have drawn attention of swine breeders due to the correlation with growth and consumer preference for carcass composition, respectively. The association between melanocortin 4 receptor (MC4R) gene, which encodes the G-protein-coupled receptor and ADG and carcass quality, have been well documented. The study was conducted to determine the effects of MC4R/TaqI and PIT-1/RsaI polymorphisms on growth and carcass quality traits in total of 576 Duroc pigs at national breeding farms of Vietnam. Methods: After the performance test, the desired traits including ADG, IF and LD were measured. ADG (g/day) was calculated as the live weight divided by the number of days from birth to 100 kg, BF and LD can be captured at position P2 (6-8 cm away from body midline at the last rib level) with Ultrasound machine Aloska SSD 500V. Genomic DNA were collected and genotyped to observe the polymorphism of MC4R and PIT-1. Result: Three genotypes AA, AG andGG of MC4R gene andAA, AB and BB of PIT-1 gene were found in the studied pig population. The observed frequencies of AA, AG and GG were 0.09, 0.41 and 0.50 (MC4R gene) and for AA, AB and BB were 0.37, 0.47 and 0.16, respectively. The G allele (MC4R) and B allele (PIT-1) have more positive effects on traits of ADG, BF and LD. Specifically, in MC4R gene, the individuals carrying GG genotype had higher ADG by 50 gram and lower BF by 1.4 mm than AA genotype. In PIT-1 gene, pigs carrying BB genotype had higher ADG and LD than AA genotype by 37 gram and by 1.9 mm, respectively. Therefore, the increased selection of G allele, GG genotype (MC4R) and of B allele, BB genotype (PIT-1) should be considered to contribute to the improvement of ADG, BF and LD traits in Duroc population in current study

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Section: Association Between Mc4r Pit-1 and Studied Traitssupporting

confidence: 91%

Polymorphisms of Candidate Genes Associated with Growth and Carcass Traits in Canadian Duroc Pigs

Tinh

Hợp

Anh

et al. 2021

IJAR

View full text Add to dashboard Cite

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“…Some studies observed an association between variants in the pGH gene with meat quality attributes (Putnová, et al, 2001;Faria et al, 2006;Bižienė et al, 2011;Lyubov, et al, 2016). Rybarczyk et al (2007), studying four SNPs, and Kmieċ et al (2010), evaluating the HaeII polymorphism, observed that this association was weak or non-existent for the characteristics associated with meat quality.…”

Section: Discussionmentioning

confidence: 99%

“…pGH has more than 300 functions (Waters et al, 2006); it is involved in the growth of skeletal muscles, bone, and adipose tissue (Parr et al, 2016;Lan et al, 2018) and stimulates the production of insulin-like growth factor 1 in the liver (Cheng et al, 2016). Studies to improve meat quality and performance characteristics with variants of the pGH gene were described by Schellander et al (1994); Knorr et al (1997); Cheng et al (2000); Song et al (2003); Franco et al (2008); Bižienė et al (2011);and Lyubov et al (2016).…”

Section: Introductionmentioning

confidence: 99%

Research Article A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs

Ferreira¹,

Braga²,

Guimarães³

et al. 2019

Genet. Mol. Res.

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Marker-assisted selection has been widely used in breeding programs. The use of single nucleotide polymorphisms (SNPs) as genetic markers enables identification of genotypes that best contribute to quantitative characteristics. We evaluated a possible association between the DdeI growth hormone gene polymorphism and meat and carcass traits. A total of 476 halothanefree animals were genotyped. Animals originated from the crossing of an AGIPIC ® 415 male with Large White (LW) x Landrace (LD) females. Males were castrated when young and males and females were slaughtered between 150 and 180 days old. Hot carcass weight, carcass length, pH 45 min , and pH 16 postmortem of Longissimus cervicis, Longissimus dorsi, and Semimembranosus muscles, backfat thickness, Longissimus dorsi muscle depth, and color by the CIELAB system were measured and the percentage of lean meat was calculated. Water holding capacity was determined by the filter paper method in the Semimembranosus muscle. A 605-base pair (bp) I.M. Ferreira et al. 2 amplicon was obtained by PCR and a DdeI polymorphism was genotyped and two alleles identified, D 1 with 335, 148, and 122 bp and D 2 allele with 457 and 148 bp. Allele frequencies were 0 . 4 3 for D 1 and 0.57 for D 2 . Genotypic frequencies were as follows: D 1 D 1 ( 22.1%), D 1 D 2 ( 49.8%), and D 2 D 2 (28.1%). The Chi-Square test showed that the population was in Hardy-Weinberg equilibrium.The results of hot carcass weight, carcass length, pH 16 postmortem of Longissimus cervicis, Longissimus dorsi, and Semimembranosus muscles, backfat thickness, Longissimus dorsi muscle depth, color and water holding capacity did not differ significantly among the genotypes. The DdeI SNP was associated with lean meat percentage and pH at 45 min posmortem in the Longissimus cervicis muscle; properties that are important for the commercialization of fresh meat as well as processed products.

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Genetic characterization of the Mirgorod pig breed, obtained by analysis of single nucleotide polymorphisms of genes

Vashchenko¹,

Balatsky²,

Pocherniaev³

et al. 2019

Agric. sci. pract.

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Aim. To determine genetic characteristics of the Mirgorod pig breed by analysis of 25 SNPs of 22 genes and to conduct the associative analysis of genes MC4R (SNP c.1426 G > A), LEP (SNP g.2845 А ˃ Т), GH (BsuRI- polymorphism), CTSF (SNP g. 22 G > C) with productive traits of animals. Methods. Blood samples of pedigree Mirgorod pigs, bred at SI «Experimental farm named after Decemberists», Poltava region, were used for the studies. DNA genotyping was performed by PCR-RFLP and TaqMan. Results. Specifi c features of the breed were determined in terms of gene allele frequencies, high level of genetic variability (He – 0.326) and allelic diversity (mean number of alleles per locus – 1.96). The KPL2/m allele that causes genetic anomaly of ISTS is absent among investigated Mirgorod pigs, and the recessive RYR1 g.1843T allele, responsible for stress sensitivity of pigs, occurs at a low frequency (0.04). Unlike other breeds, a relatively high frequency of the minor allele g.15A (0.16) of CTSK and polymorphism of the LEP gene (SNP g.3996 T > C) (He – 0.455) was observed. Statistically signifi cant associations of polymorphisms have been established: MC4R (SNP c.1426 G > A) with age of gaining 100 kg, the thickness of backfat and the Eye Muscle Area, GH/BsuRI with the age of gaining 100 kg, and CTSF (SNP g. 22G > C) with Eye Muscle Area. There was a trend of statistically signifi cant differences between groups of pigs with different genotypes of LEP (SNP g.2845 А ˃ Т) and the thickness of the backfat (p = 0.09). Conclusions. It is reasonable to carry out the restoration of the gene pool of the Mirgorod pig breed, taking into account the SNPs of the studied genes and their associations with the productive traits. It is expedient to give preference to pigs with SNP genotypes с.1426 MC4R GA, MC4R AA, g. 22 CTSF CC, g.2845 LEP TT for breed reproduction.

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Polymorphisms in Several Porcine Genes are Associated with Growth Traits

Cited by 4 publications

References 21 publications

Polymorphisms of Candidate Genes Associated with Growth and Carcass Traits in Canadian Duroc Pigs

Polymorphisms of Candidate Genes Associated with Growth and Carcass Traits in Canadian Duroc Pigs

Research Article A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs

Genetic characterization of the Mirgorod pig breed, obtained by analysis of single nucleotide polymorphisms of genes

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