Polymorphisms in Plasmodium falciparum dhfr and dhps genes and age related in vivo sulfadoxine–pyrimethamine resistance in malaria-infected patients from Nigeria

Rapid Detection of Lactate Dehydrogenase and Genotyping of Plasmodium falciparum in Saliva of Children with Acute Uncomplicated Malaria

“…Similar K76T pfcrt genotypes were observed in the parasite genomic DNA extracted from filter-paper blood samples and saliva samples. The high prevalence of the mutant pfcrt 76T allele in both saliva (57%) and blood (60%) specimens is consistent with previous reports [20][21][22] from genomic DNA extracted from blood sample.…”

“…Nested PCR with RFLP techniques are frequently used to detect polymorphisms in parasite genes that serve as molecular markers of antimalarial drug resistance. [20][21][22] This approach was used in saliva samples to detect K76T polymorphism on the pfcrt gene that is associated with chloroquine resistance. Similar K76T pfcrt genotypes were observed in the parasite genomic DNA extracted from filter-paper blood samples and saliva samples.…”

Therapeutic Efficacies of Artemisinin-Based Combination Therapies in Nigerian Children with Uncomplicated Falciparum Malaria during Five Years of Adoption as First-Line Treatments

“…The rapid progression of resistance in the late 1990s and early 2000s in P. falciparum to chloroquine and sulfadoxinepyrimethamine, [23][24][25][26] the first-and second-line treatments, respectively, of uncomplicated infections in Nigeria and the recommendation of the WHO 1 led to the introduction and adoption, in mid-2005, of AL and AA as first-line antimalarials in Nigeria. 27,28 With the adoption of these ACTs, small-scale studies have been frequent in the country, 29,30 but evaluation of relatively large numbers of children over a relatively long period is an exception.…”

Therapeutic Efficacy and Effects of Artesunate-Mefloquine and Mefloquine Alone on Malaria-Associated Anemia in Children with Uncomplicated Plasmodium falciparum Malaria in Southwest Nigeria

“…Briefly, Block 2 of merozoite surface protein-1 (MSP-1) and Block 3 of merozoite surface protein-2 (MSP-2) and region II of glutamine-rich protein (GLURP) were amplified by two rounds of polymerase chain reaction (PCR) using primers and amplification conditions described previously. [21][22][23][24] Ten microliters of the nested PCR products was resolved by electrophoresis on a 2% agarose gel and sized against a 100-bp molecular weight marker (New England Biolabs, Beverly, MA). The banding pattern of the post-treatment parasites was compared with matched primary parasites in each of the patients who had parasitemia after treatment with either artesunate-mefloquine or mefloquine.…”