Polymorphisms in Ion Transport Genes Are Associated with Eggshell Mechanical Property

Duan, Zhongyi; Chen, Sirui; Sun, Chang; Shi, Fengying; Wu, Guiqin; Liu, Aiqiao; Xu, Guiyun; Yang, Ning

doi:10.1371/journal.pone.0130160

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…The ITPR1 gene family has been found to affect eggshell thickness in a previous study by our group 28 . In current study, ITPR2, the type II isoform, was associated with EST and ET.…”

Section: Discussionmentioning

confidence: 75%

“…In this study, the ABCC9, ITPR2, KCNJ8 and WNK1 genes were associated with EST and ET, and these genes are all involved in ion transport and their expression has been detected in the uterus (Li et al in publication). Previous studies have demonstrated that ion transport plays vital roles in eggshell formation 8 28 . We speculated that more than four genes likely affected EST and ET by regulating ion transport and eggshell mineralization.…”

Section: Discussionmentioning

confidence: 99%

“…Here, we found that WNK1 affects EST and ET. Previous studies have shown that epithelial sodium channels are involved in eggshell formation and that their mutations are associated with EST or ESW (eggshell weight) 28 41 . Xu et al revealed that WNK1 increases epithelial sodium channel activity by activating PI3 kinase and stimulating the epithelial sodium channel regulator SGK1 42 .…”

Section: Discussionmentioning

confidence: 99%

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Genetic architecture dissection by genome-wide association analysis reveals avian eggshell ultrastructure traits

Duan

Sun

Shen

et al. 2016

Sci Rep

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The ultrastructure of an eggshell is considered the major determinant of eggshell quality, which has biological and economic significance for the avian and poultry industries. However, the interrelationships and genome-wide architecture of eggshell ultrastructure remain to be elucidated. Herein, we measured eggshell thickness (EST), effective layer thickness (ET), mammillary layer thickness (MT), and mammillary density (MD) and conducted genome-wide association studies in 927 F2 hens. The SNP-based heritabilities of eggshell ultrastructure traits were estimated to be 0.39, 0.36, 0.17 and 0.19 for EST, ET, MT and MD, respectively, and a total of 719, 784, 1 and 10 genome-wide significant SNPs were associated with EST, ET, MT and MD, respectively. ABCC9, ITPR2, KCNJ8 and WNK1, which are involved in ion transport, were suggested to be the key genes regulating EST and ET. ITM2C and KNDC1 likely affect MT and MD, respectively. Additionally, there were linear relationships between the chromosome lengths and the variance explained per chromosome for EST (R2 = 0.57) and ET (R2 = 0.67). In conclusion, the interrelationships and genetic architecture of eggshell ultrastructure traits revealed in this study are valuable for our understanding of the avian eggshell and contribute to research on a variety of other calcified shells.

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“…The ITPR1 gene family has been found to affect eggshell thickness in a previous study by our group 28 . In current study, ITPR2, the type II isoform, was associated with EST and ET.…”

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic architecture dissection by genome-wide association analysis reveals avian eggshell ultrastructure traits

Duan

Sun

Shen

et al. 2016

Sci Rep

Self Cite

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“…During this process, certain amounts of Ca and bicarbonate ions, and organic matrix precursors are secreted into the uterine fluid, where the organic and mineral phases interact and form the eggshell (after Duan et al . ). Especially in the biochemical/physiological context, Ca sequestration is attributable to the same ion transporter(s) binding a few different elements, which are simultaneously deposited on the outer shell membrane at the shell gland epithelial cell layer interface during shell formation (Scheuhammer , Rodriguez‐Navarro et al .…”

Section: Discussionmentioning

confidence: 97%

The effect of embryonic development on metal and calcium content in eggs and eggshells in a small passerine

Orłowski

Hałupka

Pokorny

et al. 2015

Ibis

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Published information relating to changes in the chemical element content of avian eggs caused by embryonic development is extremely scarce, although it may be crucial for understanding both the presence of anthropogenic pollutants as well as physiological levels of micronutrients. We assessed the variation in concentrations of calcium (Ca) and magnesium (Mg) and nine trace elements: seven essential (chromium (Cr), copper (Cu), nickel (Ni), manganese (Mn), iron (Fe), cobalt (Co) and zinc (Zn)) and two non-essential (lead (Pb) and cadmium (Cd)) in shells and contents (both egg yolk and egg white) of embryonated and non-embryonated eggs. We investigated the eggs of the Eurasian Reed Warbler Acrocephalus scirpaceus, a large proportion of whose eggs are infertile in our study population (almost 43% of clutches contain unhatched eggs) as well as significant embryo-induced eggshell thinning at the equator of embryonated eggs. We found significantly higher concentrations (≥ 22.7%) of all the focal elements in the contents of embryonated eggs in comparison with non-embryonated eggs, and a very pronounced one for Ca (nearly twice as high). The shells of embryonated eggs contained significantly higher concentrations of Zn (104.1%), Fe (56.5%), Pb (32.8%) and Cu (28.0%) but significantly lower ones of Co (8.9%) and Ca (9.3%) than the shells of non-embryonated eggs. The simultaneous higher concentrations of all elements in the content of thinnershelled embryonated eggs suggest the parallel transfer of these elements along with Ca resorption from the shell into the egg interior during embryo formation. The higher concentration of most elements in the thinner shells of embryonated eggs may be indicative of the maternal deposition of some of these elements in a shell layer not subject to embryonic depletion, or in the eggshell membrane. Our results highlight the need for the careful selection of egg samples, which should differentiate between embryonated and non-embryonated eggs in the analytical treatment of eggs and eggshells.

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“…Firstly, ion transporters have been suggested to be the promising candidates for the eggshell mechanical properties [ 55 ]. In this study, although a considerable number of ion transporters were differentially expressed between BSD and WSD, there is no evidence supporting that blue and white duck eggs have a significant difference in eggshell quality.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis provides clues to molecular mechanisms underlying blue-green eggshell color in the Jinding duck (Anas platyrhynchos)

et al. 2017

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BackgroundIn birds, blue-green eggshell color (BGEC) is caused by biliverdin, a bile pigment derived from the degradation of heme and secreted in the eggshell by the shell gland. Functionally, BGEC might promote the paternal investment of males in the nest and eggs. However, little is known about its formation mechanisms. Jinding ducks (Anas platyrhynchos) are an ideal breed for research into the mechanisms, in which major birds lay BGEC eggs with minor individuals laying white eggs. Using this breed, this study aimed to provide insight into the mechanisms via comparative transcriptome analysis.ResultsBlue-shelled ducks (BSD) and white-shelled ducks (WSD) were selected from two populations, forming 4 groups (3 ducks/group): BSD1 and WSD1 from population 1 and BSD2 and WSD2 from population 2. Twelve libraries from shell glands were sequenced using the Illumina RNA-seq platform, generating an average of 41 million clean reads per library, of which 55.9% were mapped to the duck reference genome and assembled into 31,542 transcripts. Expression levels of 11,698 genes were successfully compared between all pairs of 4 groups. Of these, 464 candidate genes were differentially expressed between cross-phenotype groups, but not for between same-phenotype groups. Gene Ontology (GO) annotation showed that 390 candidate genes were annotated with 2234 GO terms. No candidate genes were directly involved in biosynthesis or transport of biliverdin. However, the integral components of membrane, metal ion transport, cholesterol biosynthesis, signal transduction, skeletal system development, and chemotaxis were significantly (P < 0.05) overrepresented by candidate genes.ConclusionsThis study identified 464 candidate genes associated with duck BGEC, providing valuable information for a better understanding of the mechanisms underlying this trait. Given the involvement of membrane cholesterol contents, ions and ATP levels in modulating the transport activity of bile pigment transporters, the data suggest a potential association between duck BGEC and the transport activity of the related transporters.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4135-2) contains supplementary material, which is available to authorized users.

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Polymorphisms in Ion Transport Genes Are Associated with Eggshell Mechanical Property

Cited by 9 publications

References 42 publications

Genetic architecture dissection by genome-wide association analysis reveals avian eggshell ultrastructure traits

Genetic architecture dissection by genome-wide association analysis reveals avian eggshell ultrastructure traits

The effect of embryonic development on metal and calcium content in eggs and eggshells in a small passerine

Comparative transcriptome analysis provides clues to molecular mechanisms underlying blue-green eggshell color in the Jinding duck (Anas platyrhynchos)

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