Polymorphisms in GSTE2 is associated with temephos resistance in Aedes aegypti

Helvécio, Elisama; Romão, Tatiany Patrícia; Carvalho-Leandro, Danilo de; Oliveira, Iêda Ferreira de; Cavalcanti, Antonio Emanuel Holanda Dias; Reimer, Lisa J.; Paiva-Cavalcanti, Milena de; Oliveira, Ana Patrícia Silva de; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique; Wallau, Gabriel Luz; Neto, Osvaldo P. de Melo; Melo-Santos, Maria Alice Varjal de; Ayres, Constância Flávia Junqueira

doi:10.1016/j.pestbp.2019.10.002

Cited by 19 publications

(17 citation statements)

References 52 publications

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“…In addition, allele variants have been associated with temephos resistance in Ae . aegypti from Brazil or Thailand [ 38 ]. Interestingly, one study compared the transcription and copy number in pyrethroid-resistant strains from different continents [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death

et al. 2021

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Pyrethroids are one of the few classes of insecticides available to control Aedes aegypti, the major vector of dengue, chikungunya, and Zika viruses. Unfortunately, evolving mechanisms of pyrethroid resistance in mosquito populations threaten our ability to control disease outbreaks. Two common pyrethroid resistance mechanisms occur in Ae. aegypti: 1) knockdown resistance, which involves amino acid substitutions at the pyrethroid target site—the voltage-gated sodium channel (VGSC)—and 2) enhanced metabolism by detoxification enzymes. When a heterogeneous population of mosquitoes is exposed to pyrethroids, different responses occur. During exposure, a proportion of mosquitoes exhibit immediate knockdown, whereas others are not knocked-down and are designated knockdown resistant (kdr). When these individuals are removed from the source of insecticide, the knocked-down mosquitoes can either remain in this status and lead to dead or recover within a few hours. The proportion of these phenotypic responses is dependent on the pyrethroid concentration and the genetic background of the population tested. In this study, we sequenced and performed pairwise genome comparisons between kdr, recovered, and dead phenotypes in a pyrethroid-resistant colony from Tapachula, Mexico. We identified single-nucleotide polymorphisms (SNPs) associated with each phenotype and identified genes that are likely associated with the mechanisms of pyrethroid resistance, including detoxification, the cuticle, and insecticide target sites. We identified high association between kdr and mutations at VGSC and moderate association with additional insecticide target site, detoxification, and cuticle protein coding genes. Recovery was associated with cuticle proteins, the voltage-dependent calcium channel, and a different group of detoxification genes. We provide a list of detoxification genes under directional selection in this field-resistant population. Their functional roles in pyrethroid metabolism and their potential uses as genomic markers of resistance require validation.

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Section: Discussionmentioning

confidence: 99%

Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death

et al. 2021

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“…aegypti populations [ 35 , 67 , 68 ]. For instance, a study by Helvecio et al [ 69 ] found that several SNPs in GSTe2 correlated with temephos resistance in Ae . aegypti .…”

Section: Discussionmentioning

confidence: 99%

High DDT resistance without apparent association to kdr and Glutathione-S-transferase (GST) gene mutations in Aedes aegypti population at hotel compounds in Zanzibar

et al. 2022

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Global efforts to control Aedes mosquito-transmitted pathogens still rely heavily on insecticides. However, available information on vector resistance is mainly restricted to mosquito populations located in residential and public areas, whereas commercial settings, such as hotels are overlooked. This may obscure the real magnitude of the insecticide resistance problem and lead to ineffective vector control and resistance management. We investigated the profile of insecticide susceptibility of Aedes aegypti mosquitoes occurring at selected hotel compounds on Zanzibar Island. At least 100 adults Ae. aegypti females from larvae collected at four hotel compounds were exposed to papers impregnated with discriminant concentrations of DDT (4%), permethrin (0.75%), 0.05 deltamethrin (0.05%), propoxur (0.1%) and bendiocarb (0.1%) to determine their susceptibility profile. Allele-specific qPCR and sequencing analysis were applied to determine the possible association between observed resistance and presence of single nucleotide polymorphisms (SNPs) in the voltage-gated sodium channel gene (VGSC) linked to DDT/pyrethroid cross-resistance. Additionally, we explored the possible involvement of Glutathione-S-Transferase gene (GSTe2) mutations for the observed resistance profile. In vivo resistance bioassay indicated that Ae. aegypti at studied sites were highly resistant to DDT, mortality rate ranged from 26.3% to 55.3% and, moderately resistant to deltamethrin with a mortality rate between 79% to and 100%. However, genotyping of kdr mutations affecting the voltage-gated sodium channel only showed a low frequency of the V1016G mutation (n = 5; 0.97%). Moreover, for GSTe2, seven non-synonymous SNPs were detected (L111S, C115F, P117S, E132A, I150V, E178A and A198E) across two distinct haplotypes, but none of these were significantly associated with the observed resistance to DDT. Our findings suggest that cross-resistance to DDT/deltamethrin at hotel compounds in Zanzibar is not primarily mediated by mutations in VGSC. Moreover, the role of identified GSTe2 mutations in the resistance against DDT remains inconclusive. We encourage further studies to investigate the role of other potential insecticide resistance markers.

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“…However, there is a lack on the genotoxic effects of TM on mosquitoes in the literature. Most studies point to the regulation of expression and changes in resistance genes (Poupardin et al ., 2014; Helvecio et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and mutagenic effects of methyl ether dillapiole on the development of Aedes aegypti (Diptera: Culicidae)

Silva

Pinto

Santos

et al. 2021

Medical Vet Entomology

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Dillapiole, extracted from Piper aduncum essential oil and its derivatives, has been shown to be a potential alternative to the control of Aedes aegypti, which has become resistant to synthetic insecticides. Methyl ether dillapiole (MED) and temephos (TM) were compared to complement the data on the genotoxicity and developmental changes of Ae. aegypti. Over four generations (G 1 -G 4 ), third stage larvae were treated with MED at 60, 80 and 100 μg/mL and TM at 0.002, 0.005 and 0.007 μg/mL for 4 h. Adult females were separated to estimate oviposition and hatching rates, and total egg length. Over the four generations, a significant reduction was recorded in oviposition and hatching rates, and in mean egg length (Tukey, P < 0.05), compared with the negative control (NC). Cytological slide preparations were done from adult oocytes and larval neuroblasts. The cumulative effects of genotoxic (bridges, budding and nuclear fragmentation) and mutagenic (micronucleus and chromosomal breakage) damage was observed in the neuroblasts and oocytes of exposed mosquitoes. Developmental changes and damage to the genome of MED-treated Ae. aegypti were greater than those caused by TM. Further studies should focus on understanding the effects of the MED molecule on Ae. aegypti.

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Polymorphisms in GSTE2 is associated with temephos resistance in Aedes aegypti

Cited by 19 publications

References 52 publications

Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death

Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death

High DDT resistance without apparent association to kdr and Glutathione-S-transferase (GST) gene mutations in Aedes aegypti population at hotel compounds in Zanzibar

Genotoxic and mutagenic effects of methyl ether dillapiole on the development of Aedes aegypti (Diptera: Culicidae)

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