Polymorphisms in equine immune response genes and their associations with infections

Hořín, Petr; Smoła, J.; Matiašovic, Ján; Vyskočil, M.; Lukeszová, L; Tomanová, K.; Králík, Petr; Glasnák,; Schröffelová, Daniela; Knoll, A.; Sedlinská, Markéta; Krenková, L; Jähn, Peter S.

doi:10.1007/s00335-004-2356-6

Cited by 29 publications

(20 citation statements)

References 28 publications

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“…In our study, no genomic regions associated with IBH were found on ECA14. Hořin et al [46] investigated polymorphisms in various immune response related genes to identify associations with R . equi and L .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study of insect bite hypersensitivity in two horse populations in the Netherlands

et al. 2012

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BackgroundInsect bite hypersensitivity is a common allergic disease in horse populations worldwide. Insect bite hypersensitivity is affected by both environmental and genetic factors. However, little is known about genes contributing to the genetic variance associated with insect bite hypersensitivity. Therefore, the aim of our study was to identify and quantify genomic associations with insect bite hypersensitivity in Shetland pony mares and Icelandic horses in the Netherlands.MethodsData on 200 Shetland pony mares and 146 Icelandic horses were collected according to a matched case–control design. Cases and controls were matched on various factors (e.g. region, sire) to minimize effects of population stratification. Breed-specific genome-wide association studies were performed using 70 k single nucleotide polymorphisms genotypes. Bayesian variable selection method Bayes-C with a threshold model implemented in GenSel software was applied. A 1 Mb non-overlapping window approach that accumulated contributions of adjacent single nucleotide polymorphisms was used to identify associated genomic regions.ResultsThe percentage of variance explained by all single nucleotide polymorphisms was 13% in Shetland pony mares and 28% in Icelandic horses. The 20 non-overlapping windows explaining the largest percentages of genetic variance were found on nine chromosomes in Shetland pony mares and on 14 chromosomes in Icelandic horses. Overlap in identified associated genomic regions between breeds would suggest interesting candidate regions to follow-up on. Such regions common to both breeds (within 15 Mb) were found on chromosomes 3, 7, 11, 20 and 23. Positional candidate genes within 2 Mb from the associated windows were identified on chromosome 20 in both breeds. Candidate genes are within the equine lymphocyte antigen class II region, which evokes an immune response by recognizing many foreign molecules.ConclusionsThe genome-wide association study identified several genomic regions associated with insect bite hypersensitivity in Shetland pony mares and Icelandic horses. On chromosome 20, associated genomic regions in both breeds were within 2 Mb from the equine lymphocyte antigen class II region. Increased knowledge on insect bite hypersensitivity associated genes will contribute to our understanding of its biology, enabling more efficient selection, therapy and prevention to decrease insect bite hypersensitivity prevalence.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association study of insect bite hypersensitivity in two horse populations in the Netherlands

et al. 2012

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“…More work is needed to understand the risk factors for the development of N‐EPE, which could include environmental/husbandry differences, genetic polymorphisms, the infectious dose, L. intracellularis strain, concurrent disease, or animal's immune status. Our previous work with an experimental challenge model seems to suggest that the host immune system likely plays the largest role in this type of clinical presentation as the infectious dose and strain used in that study was consistent across 6 challenged weanlings, yet only 1 developed N‐EPE; however, based on the occurrence of 2 N‐EPE cases on the same farm in consecutive years, a significant role of bacterial strain cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Acute Deterioration and Death with Necrotizing Enteritis Associated withLawsonia intracellularisin 4 Weanling Horses

Page

Fallon

Bryant

et al. 2012

Veterinary Internal Medicne

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“…Frequencies of variants (not in parenthesis) are shown for each breed/species and dashes indicate the variant is not found in a given breed/species. Breed and susceptibility to R equi pneumonia, 30 but this study used a PCR restriction fragment length polymorphism method permitting detection of only 4 of the variants in the 59UTR region, not including the -57C/T polymorphism.…”

Section: Discussionmentioning

confidence: 99%

“…polymorphism involved in natural resistance to R equi pneumonia, because the disease was detected in foals from Farm A that were fixed for the -57C allele (presumptively associated with foals exposed but not infected with R equi pneumonia based on the data from Farms B and C; Table 3). Two other equine gene regions have been associated with the presence of high numbers of R equi in TBA fluid, 30…”

Section: Discussionmentioning

confidence: 99%

“…19 The structure of the SLC11A1 gene in horses is similar to that in other mammals, including 15 exons corresponding to a putative protein of 544 amino acids. 28 To date, single nucleotide polymorphisms (SNPs) have been identified in the horse SLC11A1 intron 5 29,30 and 59untranslated region (UTR). 28 To our knowledge, SLC11A1 polymorphisms have not been investigated in non-horse equid species to date.…”

Section: Iseases Caused By Intracellular Bacteria Such Asmentioning

confidence: 99%

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Variations in Equid SLC11A1 (NRAMP1) Genes and Associations with Rhodococcus Equi Pneumonia in Horses

Halbert¹,

Cohen²,

Slovis³

et al. 2006

J Vet Int Med

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Rhodococcus equi is an important intracellular pathogen of horses, most commonly causing chronic, suppurative bronchopneumonia in foals. Although most foals likely are exposed to environmental R. equi within the 1st few days of life, only some develop R. equi pneumonia, and the basis of differences in susceptibility among foals currently is unknown. In this study, we investigated solute carrier family 11 member 1 (SLC11A1) gene sequences in the 5' untranslated region, exon 1, and a portion of intron 1 for variations in 3 equid species (horse, donkey, zebra) and compared variants within 3 independent horse breeding farms for associations with R. equi pneumonia by use of an age-matched case-control design. Seven novel variants in the 5'untranslated region were identified as specific for one or both of the non-horse equid species sampled. In addition, a single novel horse variant in the 5'untranslated region, -57C/T, was identified in 4 breeds. The -57C/T variant was found on 2 of the 3 farms with endemic R. equi pneumonia, representing 2 different horse breeds. Significant allelic and genotypic associations with susceptibility to R. equi pneumonia were observed for the -57C/T variant in foals from these farms. Although the functional impact of this novel variant remains to be determined, this study represents an important step in our understanding of natural resistance to R. equi foal pneumonia and other intracellular bacterial diseases affecting equids.

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Polymorphisms in equine immune response genes and their associations with infections

Cited by 29 publications

References 28 publications

Genome-wide association study of insect bite hypersensitivity in two horse populations in the Netherlands

Genome-wide association study of insect bite hypersensitivity in two horse populations in the Netherlands

Acute Deterioration and Death with Necrotizing Enteritis Associated withLawsonia intracellularisin 4 Weanling Horses

Variations in Equid SLC11A1 (NRAMP1) Genes and Associations with Rhodococcus Equi Pneumonia in Horses

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