Polymorphisms and haplotypes of TLR-4/9 associated with bacterial infection, gingival inflammation/recession and oral cancer

Jha, Arpita; Nath, Nidhi; Kumari, Archana; Kumari, Nidhi; Panda, Aditya K.; Mishra, Rajakishore

doi:10.1016/j.prp.2022.154284

Cited by 6 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, TC and CC genotype and C allele of TLR9 rs187084, as well as TLR9 haplotypes containing TLR9 rs187084 C allele were associated with increased OSCC risk, while haplotypes containing T allele may play a protective role [25]. A large meta-analysis study showed that the investigated TLR9 polymorphism rs187084 was significantly associated with an increased risk of overall cancer, particularly cervical cancer, whereas the different polymorphisms rs352140 and rs5743836 in the TLR9 gene exerted a protective role in the development of breast and digestive cancers [39].…”

Section: Discussionmentioning

confidence: 93%

“…Given that immune pathways impact cancer susceptibility, progression, survival, and therapy, there is growing interest in pharmacological targeting of TLRs and the role of their genetic polymorphisms in cancer. However, there are limited data on TLR7 and TLR9 polymorphisms associated with OSCC [25] and HNSCC [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

“…While our study did not show the association of TLR7 and TLR9 polymorphisms with OSCC risk, several studies on oral cancer and other tumors types demonstrated their association with cancer susceptibility. A study on TLR9 polymorphisms rs5743836, rs352140, rs187084 and rs352139 in OSSC patients found that all investigated variants could increase the oral cancer risk and potentiate bacterial gingival inflammation/gingival recession [25].…”

Section: Discussionmentioning

confidence: 99%

“…However, there are limited data on the association between TLR7 and TLR9 polymorphisms and prognosis or susceptibility to oral cancer [24,25] or other subtypes of head and neck squamous cell carcinoma (HNSCC), such as nasopharyngeal carcinoma (NPC) [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Impact of TLR9 and TLR7 gene polymorphisms on prognosis and survival of patients with oral squamous cell carcinoma

Obrenović,

Šupić,

Miyabe

et al. 2024

Biomol Biomed

View full text Add to dashboard Cite

Despite significant efforts in developing new diagnostic and therapeutic modalities, oral squamous cell carcinomas (OSCCs) still exhibit a high recurrence rate, a low five-year survival rate, and an increasing prevalence. Toll-like receptors (TLRs), which initiate and perpetuate immune mechanisms upon activation, have been linked to immune surveillance and the antitumor immune response. The aim of this study was to investigate the association between the polymorphisms of the TLR7 rs3853839 and TLR9 rs187084 genes and OSCC risk, clinicopathological features, and survival. Genotyping was assessed by real-time polymerase chain reaction (PCR) in 95 HPV-negative OSCC patients and 107 age- and sex-matched healthy controls. Patients with lymph node metastases had higher frequencies of the TLR9 rs187084 CC variant genotype compared to the major TT genotype (P = 0.020) and to T-allele carriers (combined TT + CT genotypes, P = 0.015). A higher prevalence of advanced stage III was observed in patients with the TLR9 rs187084 variant CC genotype compared to TT (P = 0.047) and to T-allele carriers (TT + CT, P = 0.037). Kaplan-Meier analysis revealed a lower overall survival rate in patients with the TLR9 rs187084 variant CC genotype compared to TT genotype (P = 0.010, log-rank test) and to T-allele carriers (TT + CT, P = 0.002), though it was not an independent predictor of overall survival. Both TLR9 rs187084 and TLR7 rs3853839 polymorphisms were associated with high alcohol consumption (P = 0.027 and P = 0.001, respectively). The investigated genetic variations were not associated with OSCC susceptibility. The variant CC genotype of the TLR9 rs187084 polymorphism might be a marker of poor survival and tumor progression in OSCC.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of TLR9 and TLR7 gene polymorphisms on prognosis and survival of patients with oral squamous cell carcinoma

Obrenović,

Šupić,

Miyabe

et al. 2024

Biomol Biomed

View full text Add to dashboard Cite

show abstract

“…They assessed several SNPs of TLR4 and TLR9 genes with respect to different phenotypes such as bacterial infection, gingival inflammation/recession, and OSCC. They employed techniques such as RT-PCR, Western blot analysis, allele-specific PCR, and sequencing to investigate the same [ 28 ]. The present study was an attempt to provide an answer to the research question about the association between the TLR4 gene polymorphism (rs4986790) and the susceptibility to OSCC and how this genetic variant contributes to the risk and progression of OSCC.…”

Section: Discussionmentioning

confidence: 99%

Genetic Association of Toll-Like Receptor 4 (TLR4) Gene Polymorphism (rs4986790) With Oral Squamous Cell Carcinoma (OSCC): A Pilot Case-Control Study

Gautam,

Pandi,

Girija

et al. 2024

Cureus

View full text Add to dashboard Cite

Introduction Oral squamous cell carcinoma (OSCC) is a highly prevalent and most common form of oral malignancy in the Indian population. Toll-like receptors belong to an important family of receptors that are involved in the process of pathogen recognition and mounting immune response. The expression of this receptor is dysregulated on the tumor cells as reported across several cancer types. The genetic variants in this gene could have a profound impact on the expression of the Toll-like receptor 4 ( TLR4) gene. Objective This study aimed to understand the association of TLR4 gene polymorphism (rs4986790) with OSCC. The objective of this study was to compare the allele and genotype frequencies between the two groups, viz., OSCC and normal healthy subjects, recruited in the study. Materials and methods The blood samples were collected from normal healthy subjects (N = 25) and OSCC patients (N = 25). Genomic DNA was isolated from all samples, and genotyping was performed for the TLR4 gene polymorphism (rs4986790) employing the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The frequency distribution of genotypes and alleles across the study groups was determined by the Chi-square test. Results The allele frequency for TLR4 gene polymorphism (rs4986790) in the case group was found to be 60% (A allele) and 40% (G allele), respectively. The study population in both groups were found to agree with the Hardy-Weinberg equilibrium (HWE). The genotype frequency did not differ significantly among the two study groups which was evident from the p-value = 0.8285. Conclusion The present study did not report any significant association of the TLR4 polymorphic marker rs4986790 with OSCC. Further investigations into the association of other polymorphic markers in the TLR4 gene, among the larger population of OSCC patients, could provide evidence of their association with OSCC.

show abstract

Role of toll-like receptor in the pathogenesis of oral cancer

Bhardwaj,

Prasad,

Mukherjee

2023

Cell Biochem Biophys

View full text Add to dashboard Cite

Polymorphisms and haplotypes of TLR-4/9 associated with bacterial infection, gingival inflammation/recession and oral cancer

Cited by 6 publications

References 59 publications

Impact of <i>TLR9 </i>and <i>TLR7</i> gene polymorphisms on prognosis and survival of patients with oral squamous cell carcinoma

Impact of <i>TLR9 </i>and <i>TLR7</i> gene polymorphisms on prognosis and survival of patients with oral squamous cell carcinoma

Genetic Association of Toll-Like Receptor 4 (TLR4) Gene Polymorphism (rs4986790) With Oral Squamous Cell Carcinoma (OSCC): A Pilot Case-Control Study

Role of toll-like receptor in the pathogenesis of oral cancer

Contact Info

Product

Resources

About