Polymorphism study of nine SNPs associated with subjective response to alcohol in Chinese Han, Hui, Tibetan, Mongolian and Uygur populations

Abstract

Heavy alcohol drinking is a major public health problem, causing a large disease, social and economic burden in societies. Subjective response (SR) to alcohol is an intermediate characteristic of heavy drinking. A variety of candidate genes have been reported to be associated with SR to alcohol. In this study, we investigated nine single nucleotide polymorphisms (SNPs) related to SR to alcohol in healthy individuals from five Chinese ethnic groups, the Han, Hui, Tibetan, Mongolian and Uygur populations, and a total of 584 bloodstain samples were collected. The nine SNPs included four SNPs in alcohol-metabolizing genes (ADH1B, ADH1C, ALDH2 and CYP2E1*5B) and five SNPs in genes of neurobiological pathways (GABRA2, OPRM1, CHRNA3, HYKK and SLC6A4). A SNaPshot analysis method was developed to type these SNPs simultaneously, and all samples were typed successfully. Statistical analyses of the allele frequencies indicated that the frequencies of all SNPs, except for ADH1C, showed varying degrees of difference in the five studied ethnic groups. Tibetans showed the highest frequencies of risk alleles for heavy drinking at most loci. The genetic polymorphic differences found in this study revealed the variation in genetic susceptibility to heavy drinking in the studied populations.

Association between ALDH2 Variant and Alcohol Dependence in South Indian Population.

“…However, Vaswani et al found the ALDH2*2 minor allele to be present at a frequency of 19% in a north Indian population which agrees with the nding of the present study [29].The minor allele frequency of rs698 in this study population is marginally higher than north Indians (25%) and south West Indians (27%) [14,30]. Across the major global populations, the frequency of this allele is seen at 50% in Caucasians and 15% in African Americans [14] and is the least frequent in east Asians [16,31].…”

Association of ADH1C and ALDH2 genes with alcohol dependence in south Indian Tamilian population

“…The frequency of rs671 across Asian control populations (24% Japanese, 27% southern Han Chinese, 36% Koreans, 30% Taiwanese) is found to be higher when compared to our study control population [24][25][26]. Interestingly, there exists a wide variation in MAF among five Chinese ethnic populations and the frequency of A allele ranges between 0.8% to 18.9% [27]. Among Indian populations, intriguingly a study reported Glu504 to be monomorphic in some Indian populations (Madhya Pradesh, Maharashtra and Andhra Pradesh) among healthy controls [28].…”

“…On comparing the control population, the MAF of rs698 in the present study is marginally higher than south west American Indians (n=72, 27%) and north Indians (n= 108, 25%) [11,32]. Across the major global populations, the frequency of this allele is seen at 50% in Caucasians and 15% in African Americans and is the least frequent in east Asians [11,13,27]. In our study, minor allele G is found to be slightly higher in alcoholics than control population though there is no significant difference seen in the allele frequencies between these groups.…”

Differences in the Association Between Modifiable Lifestyle Factors and Gastric Precancerous Lesions Among Mongolians and Han Chinese

“…First, ALDH2*G homozygote, a genetic variation associated with heavy drinking, has been found to be more prevalent among Mongolians than Han Chinese ( 22 ). A population based study from Japan also showed that individuals with ALDH2 typical homozygote consumed significantly larger quantities of ethanol than those with ALDH2 heterozygote ( 23 ).…”