Polymorphism of SSR alleles in pear cultivars grown in Belarus

Urbanovich, O. Yu.; Kazlouvskaya, Z. A.; YAKIMOVICH, O. A.; Kartel, N. A.

doi:10.1134/s1022795411030173

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…Overall, the average number of alleles (17) among the analysed genotypes was increased in this study compared with that observed in earlier studies performed in several collections of P . communis using different sets of SSRs (ranging from 5.9 to 12.8) [ 21 , 25 , 54 , 56 – 57 , 61 – 66 ].…”

Section: Discussionmentioning

confidence: 99%

Elucidating the contribution of wild related species on autochthonous pear germplasm: A case study from Mount Etna

et al. 2018

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The pear (genus Pyrus) is one of the most ancient and widely cultivated tree fruit crops in temperate climates. The Mount Etna area claims a large number of pear varieties differentiated due to a long history of cultivation and environmental variability, making this area particularly suitable for genetic studies. Ninety-five pear individuals were genotyped using the simple sequence repeat (SSR) methodology interrogating both the nuclear (nDNA) and chloroplast DNA (cpDNA) to combine an investigation of maternal inheritance of chloroplast SSRs (cpSSRs) with the high informativity of nuclear SSRs (nSSRs). The germplasm was selected ad hoc to include wild genotypes, local varieties, and national and international cultivated varieties. The objectives of this study were as follows: (i) estimate the level of differentiation within local varieties; (ii) elucidate the phylogenetic relationships between the cultivated genotypes and wild accessions; and (iii) estimate the potential genetic flow and the relationship among the germplasms in our analysis. Eight nSSRs detected a total of 136 alleles with an average minor allelic frequency and observed heterozygosity of 0.29 and 0.65, respectively, whereas cpSSRs allowed identification of eight haplotypes (S4 Table). These results shed light on the genetic relatedness between Italian varieties and wild genotypes. Among the wild species, compared with P. amygdaliformis, few P. pyraster genotypes exhibited higher genetic similarity to local pear varieties. Our analysis revealed the presence of genetic stratification with a ‘wild’ subpopulation characterizing the genetic makeup of wild species and the international cultivated varieties exhibiting the predominance of the ‘cultivated’ subpopulation.

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Section: Discussionmentioning

confidence: 99%

Elucidating the contribution of wild related species on autochthonous pear germplasm: A case study from Mount Etna

et al. 2018

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“…'Fuji' showed a high level of admixture and had large differences from other members of their species. It is reported that the pedigree of 'Duchesse' pear is not clear [49]. In addition, the pattern of phylogenetic distribution of different genera in this investigation based on RAPD markers clearly revealed the differences between the studied genera ( Figure 5).…”

Section: Population Genetic Structurementioning

confidence: 62%

Phylogenetic analysis among some pome fruit trees of Rosaceae family using RAPD markers

Zarei

Erfani‐Moghadam

Mozaffari

2017

Biotechnology & Biotechnological Equipment

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To evaluate the phylogenetic relationships among subtribe Pyrinae of Rosaceae, 50 different genotypes and cultivars of pome fruit trees were collected from various locations in Iran, and analysed using random amplified polymorphic DNA (RAPD) markers. Altogether, 85 polymorphic fragments were produced by 11 random 10-mer primers. The number of produced bands varied from 4 to 12 for each primer, 7.72 on average. The lowest Jaccard's genetic similarity coefficient was scored between apple cv. 'Akan' and a wild Pyrus syriaca pear (0.04), and the highest similarity was observed between two genotypes of P. syriaca (0.97). Cluster analysis using the unweighted pair group method with arithmetic mean (UPGMA) properly separated the accessions and divided them into three major groups, including Malus, Pyrus, Mespilus and Crataegus. Members of Mespilus and Crataegus were further separated and formed two subgroups. Analysis of the genetic structure, using STRUCTURE and phylogenetic relationship using TreeView software confirmed the results of the UPGMA dendrogram and properly distinguished different genera. Among different genera studied in our experiment, Malus was the most distinct one and showed high genetic distance from other genera. Generally, the results from the present study provide valuable information about the genetic relationship among important pome fruit trees in Iran and indicate that, although Mespilus is genetically close to the Crataegus genus, it has relatively high similarity with Pyrus and Malus. Moreover, outcomes of this research confirmed that RAPD molecular markers are an appropriate means for evaluation of the genetic relationship among various genera of pome fruit trees.

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“…Screenings based on the SSR have been performed for various large collections of European pear (Ahmed et al 2010, Bassil and Postman 2009, Bassil et al 2008, Brini et al 2008, Fernández-Fernández 2010, Miranda et al 2010, Sisko et al 2009, Urbanovich et al 2011, Wünsch and Hormaza 2007, Xuan 2008, Yakovin et al 2011). However, most of the studies showed a large number of mislabelling even in well-characterized fruit tree collections (Schouten et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of pear cultivars using SSR markers and their reactions to pear rust (Gymnosporangium fuscum)

Kaymak

Pınar

2020

Bitki Koruma Bülteni

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Polymorphism of SSR alleles in pear cultivars grown in Belarus

Cited by 13 publications

References 29 publications

Elucidating the contribution of wild related species on autochthonous pear germplasm: A case study from Mount Etna

Elucidating the contribution of wild related species on autochthonous pear germplasm: A case study from Mount Etna

Phylogenetic analysis among some pome fruit trees of Rosaceae family using RAPD markers

Genetic diversity of pear cultivars using SSR markers and their reactions to pear rust (Gymnosporangium fuscum)

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