Polymorphism of MTHFR C677T Gene and the Associations with the Severity of Essential Hypertension in Northern Chinese Population

Abstract

Objective. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme of homocysteine metabolism and is closely related to the occurrence of hypertension. The aim of this study was to investigate the polymorphism of the (MTHFR) C677T and the factors influencing the severity of hypertension. Material and Methods. A total of 985 subjects were enrolled to analyze the polymorphisms of the MTHFR C677T gene by polymerase chain reaction (PCR). 306 people with essential hypertension were selected from 985 subjects to estimate the severity of hypertension by the ordinal multivariate logistical regression model. Results. The frequencies of CC, CT, and TT genotypes were 19.5%, 49.95%, and 30.46%, respectively. The allelic frequency of mutant T was 55.43%. The plasma homocysteine level of the homozygous TT in individuals was significantly higher than in those with CC or CT P < 0.01 . MTHFR677CT genotype, MTHFR677TT genotype, smoking, family history of hypertension, Hcy, and triglycerides (TG) were independent risk factors for the severity of hypertension (OR = 2.29, 2.24, 2.04, 1.81, 1.04, 1.26). Conclusion. MTHFR gene, smoking, family history of hypertension, Hcy, and triglycerides could be important genetic and high-risk factors of the development of severe hypertension in northern Chinese. These factors will contribute to the identification of high-risk populations of hypertension and facilitate the development of hypertension control strategies.

Explore the Role of the rs1801133-PPARG Pathway in the H-type Hypertension

“…There was no difference in the distribution of rs1801133 genotypes based on sex and ethnicity ( p value > 0.05), which was consistent with the report from the majority of previous studies [ 11 ]. However, we noted that patients in TT genotype were significantly younger than patients in CC or CT genotype group ( p value < 0.05).…”

“…Hcy is a sulfur-containing amino acid derived from the metabolism of methionine and is metabolized by one of the two pathways: remethylation or transsulfuration. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme of homocysteine metabolism, providing a methyl group for Hcy remethylation into methionine and maintaining the normal levels of Hcy in the body [ 11 ]. Mutations of the MTHFR gene decrease MTHFR enzyme activity that prevents Hcy remethylation and increases Hcy levels in plasma [ 11 , 12 ].…”

“…Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme of homocysteine metabolism, providing a methyl group for Hcy remethylation into methionine and maintaining the normal levels of Hcy in the body [ 11 ]. Mutations of the MTHFR gene decrease MTHFR enzyme activity that prevents Hcy remethylation and increases Hcy levels in plasma [ 11 , 12 ]. The MTHFR gene has at least two functional polymorphisms, 677T and 1298C.…”

“…However, the results obtained remained controversial. For example, one recent clinical study involving 241 cases found a significant higher TT genotype prevalence in H-type hypertension patients (Hcy ≥ 15 μ mol/L) than that in non-H-type hypertension patients (Hcy < 15 μ mol/L), suggesting that TT variant of rs1801133 could be associated with H-type hypertension [ 11 ]. However, another recent study involving 185 patients found no association between the rs1801133 polymorphism and H-type hypertensive [ 8 ].…”

Protective effect of metafolin in pregnant patients with MTHFR polymorphism, under heparin and aspirin protect treatment

“…For his part, Karakantza et al, 31 in a study that included 15 women with PA, report high incidence of the MTHFR C677T mutation (40%), similar to Gebhardt et al, 32 which included 18 women with PA, found MTHFR C677T prevalence of 28%. In parallel, Song et al, 33 report in a group of 306 patients with hypertension MTHFR-C677T prevalence of 77.7%. In turn, Ya et al, 34 in systematic review, found prevalence of 2.6 to 47.06% of the MTHFR-C677T polymorphism in 8064 women with hypertension.…”