Polymorphism of &lt;i&gt;CAST, GH, GDF9&lt;/i&gt; genes of Gorno-Altai sheep breed

Aim. One of the important tasks in solving the problem of improving the efficiency of sheep breeding is the rational use of fodder lands in the North Caucasus and the South of Russia, a significant part of which is located in different natural and climatic conditions. The complexity of solutions lies in insufficient knowledge of the mechanisms of adaptation of farm animals, in particular sheep, to the ecological and geographical features of these regions. The purpose of this research was to study the genetic polymorphism of genes, the degree of genetic variability and immune status in sheep of different breeds, since genetic polymorphism is a measure of its adaptability and the hematopoietic function of blood is the basis of the life of the organism.Material and Methods. The genetic structure of the GH/Hae III, GDF9/AsplEI genes and immune status, was studied by the methods of genetic‐statistical and immune analysis in sheep of different breeds kept in different climatic zones of Dagestan and the Krasnodar Territory.Results. As a result of DNA genotyping of the studied samples of sheep of different breeds, it was found that polymorphism of GH/HaeIII, GDF9/AsplEI genes is represented by three genotype variants: homozygous GH/HaeIIIАА, GH/HaeIIIВВ; GDF9/AsplEIАА, GDF9/AsplEIGG; heterozygous GH/HaeIIIAB; GDF9/AsplEIAG and two alleles: A and B – of the GH/HaeIII gene; A and G – GDF9/AsplEI gene with different frequency of occurrence, respectively.Conclusion. For the first time, genetic variability has been studied in the context of the immune status of sheep populations of different breeds bred in the foothills of the Republic of Dagestan and Krasnodar Territory. The information obtained about the role of the degree of genetic variability and the immune status of sheep of different breeds is answered in the formation of adaptive‐compensatory mechanisms to specific conditions of the breeding environment. A deeper study of the adaptive characteristics of sheep of different breeds for their further improvement allows the use of molecular genetic and hematological methods.

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Distribution of genotypes by the growth diferential factor 9 (GDF-9) gene locus in the romanov breed sheep population

Klimanova,

Konovalova,

Korotkevich

et al. 2024

Vestn. Novosib. gos. agrar. univ.

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The FecG gene, also known as GDF-9 (growth factor differentiation factor 9), plays an important role in regulating reproductive function in mammals. This paper presents the results of a study of a point mutation in the differential growth factor gene in Romanov breed sheep. Mutations in exon 1 of the GDF-9 gene can lead to disturbances in ovarian development and ovulation, resulting in infertility or reduced reproductive capacity in animals. The object of the study was Romanov breed sheep in a sample of 30 individuals. For DNA extraction, venous blood samples were taken from the jugular vein. In Romanov breed sheep for the GDF-9 gene, it was found that at the G1 locus of this gene there are two alleles – G and A. Three genotypes were also identified: GG (254 bp/117 bp), AG (410bp/254/117 bp) and AA (410 bp/410 bp). The observed χ2 criterion was less than expected, therefore, the empirical and theoretical frequencies do not differ significantly, and deviations from the distribution according to the Hardy-Weinberg law are not observed. The observed heterozygosity index (Ho) was 0.27. Allele frequencies were 0,8 for G and 0,2 for A. For genotypes the following were obtained: GG – 0,66, GA – 0,27 and AA – 0,07.It can be noted that the data on the frequencies of alleles and genotypes in the population of Romanov breed sheep differ from the results for the Kulunda fine-wool, Dagestan mountain, Salsk, Lacaune and Merino breeds. The research results can be used to monitor changes in allele frequencies and genotypes in sheep during the selection process.

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Polymorphism of <i>CAST, GH, GDF9</i> genes of Gorno-Altai sheep breed

Cited by 4 publications

References 7 publications

Association of Growth Hormone (GH) Gene Polymorphism with Biological Features in Sheep in Conditions of Sustainable Development of the Agricultural System

Association of Growth Hormone (GH) Gene Polymorphism with Biological Features in Sheep in Conditions of Sustainable Development of the Agricultural System

Polymorphism of <i>GH/HaeIII</i> and <i>GDF9/AsplEI</i> genes, genetic variation and association of their genotypes with immune status in sheep of different breeds ranched in different natural and geographic areas

Distribution of genotypes by the growth diferential factor 9 (GDF-9) gene locus in the romanov breed sheep population

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