Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico

Abstract

The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua F IS = 0.1 (p = 0.019) and Tamaulipas F IS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations.

Genetic diversity and population genetic analysis of bovine MHC class II DRB3.2 locus in three Bos indicus cattle breeds of Southern India

“…Similar phylogenetic analysis utilizing BoLA‐DRB3.2 allele frequencies was previously reported to establish evolutionary relationships among various taurine , Creole and indicus breeds (Takeshima et al. , 2003; Fernández et al. , 2008; Behl et al.…”

“…Putative evolutionary relationships among different populations can be determined from the genetic distances derived from frequencies of alleles (Mizuki et al, 1997). Similar phylogenetic analysis utilizing BoLA-DRB3.2 allele frequencies was previously reported to establish evolutionary relationships among various taurine, Creole and indicus breeds (Takeshima et al, 2003;Ferná ndez et al, 2008;Behl et al, 2009).…”

Association of BoLA-DRB3 genotype with somatic cell count in milk of Polish Holstein cattle

“…BoLA-DRB3 is the most polymorphic gene in cattle major histocompatibility complex (MHC); hence, it has been the subject of intensive research for more than twenty years. So far, most of the reports concern the expression and polymorphism of BoLA-DRB3 (van Eijk et al, 1992;Dietz et al, 1997a,b;Sharif et al, 1998;Gillespie et al, 1999;Takeshima et al, 2003;Fernández et al, 2008;Hameed et al, 2008;Duangjinda et al, 2009;Juliarena et al, 2009). Even though the entire BoLA-DRB3 gene was successfully sequenced in the early 90s of the twentieth century (Groenen et al, 1990), a complete structure of BoLA-DRB3 gene including the length of coding and noncoding sequences was presented after over 10 years (Russell et al, 2004).…”

Polimorfismos del gen BoLA-DRB3.2* en ganado criollo colombiano

“…El GCC compartió al menos un alelo con frecuencia mayor al 5% con otras razas criollas genotipificadas, el alelo *37 lo compartió con el Saavedreño (18), el *20 y *24 con el criollo argentino (19), con los criollos mexicanos Chihuahua y Tamaulipas el *23 y el *24 (17). No se encontraron alelos de alta frecuencia compartidos con el criollo uruguayo (20).…”